Results 191 to 200 of about 13,659,719 (307)

FOXQ1 activates GLT8D2 to enhance CCL2 N-glycosylation and promote prostate cancer bone metastasis. [PDF]

Cell Death Dis
Xie Z, Peng N, Zhao L, Zhen C, Song XL, Hong Y, Li Q, Xie T, Peng T, Zeng X, Cen J, Ge S, Liu X, Feng N, Chen M, Zhao SC.   +15 more
europepmc   +1 more source

Unmasking Glycoforms: Lectin‐Based Profiling and Functional Implications of Targeted Glycosylation Knockouts in Chinese Hamster Ovary Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Monoclonal antibody (mAb) glycosylation is a critical quality attribute that is difficult to rationally engineer and rapidly assess during cell line development. Here, we investigate whether cell‐surface glycosylation can serve as a surrogate readout of mAb product glycosylation following targeted glycogene engineering in CHO cells.
Cristina Abascal Ruiz, Sheryl Li Yan Lim, Jacobus Brink, Sara Carillo, Eoin Casey, Jonathan Bones, Ioscani Jiménez del Val   +6 more
wiley   +1 more source

IgG N-Glycosylation and Incident Neuropathy and Nephropathy in People With Type 2 Diabetes: A Pooled Analysis of Prospective Cohort Studies. [PDF]

Diabetes Care
Martinez Escobedo C, Birukov A, Heijmans R, Kuxhaus O, Eichelmann F, Slieker RC, Memarian E, Simunic-Briski N, Rutters F, Blom MT, Riera M, Crespo M, Wuhrer M, Barrios C, van Hoek M, 't Hart LM, Sijbrands E, Lauc G, Schulze MB, Wittenbecher C.   +19 more
europepmc   +1 more source

GlycoMAC: A Multiscale Metabolic‐Glycosylation Framework for Predicting Glycosylation Across Conditions in Mammalian Cell Cultures

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Antibody productivity and glycosylation quality in CHO cell cultures emerge from a dynamically evolving metabolic environment, yet existing models often work in isolation or at a single scale. Here, we present a multiscale mechanistic framework linking molecular, cellular, and macroscopic scales to predict how inputs shape bioprocess ...
Yuming Zeng, Sarah W. Harcum, Jinxiang Pei, Wei Xie   +3 more
wiley   +1 more source

Glycoproteomic and genetic analysis of N-glycosylation of complement component C3 reveals immune pathway regulation. [PDF]

Genome Biol
Šoić D, Rudman N, Grant OC, Vučković F, Frkatović-Hodžić A, Gazaway E, Pociot F, Wilson JF, Polašek O, Woods RJ, Gornik O.   +10 more
europepmc   +1 more source

Next‐Generation Glutamine Synthetase Expression Vector for Therapeutic Protein Manufacturing Using CHO Cells

Biotechnology and Bioengineering, EarlyView.
ABSTRACT The glutamine synthetase expression system is widely used for therapeutic protein biomanufacturing in Chinese hamster ovary (CHO) cells. Here, we present the design of an enhanced glutamine synthetase vector for generating CHOK1SV GS‐KO stable pools that produce recombinant proteins at multigram‐per‐liter levels. We first developed a synthetic
Yusuf B. Johari, Sophie J. Wensley, Malak A. Ammar, Avinash Srivastava, Changhavi Ragurajan, Jenish Patel, Natalie C. Elliott, Alice C. Hodgson‐Casson, Mariano Olivera Fedi, Michael Anbar, Peter M. O'Callaghan, Bernie Sweeney   +11 more
wiley   +1 more source

Integrative Quantitative Proteomics and N-Glycoproteomics Reveal Age-Processing of N-Glycosylation in Caenorhabditis elegans. [PDF]

Proteomics
Hu X, Xiang W, Qin S, Zhang X, Tian Z.
europepmc   +1 more source

Biomaterials for modulating cellular responses of endogenous tendon stem/progenitor cells: A promising avenue for tendon regeneration

BMEMat, EarlyView.
This review comprehensively summarizes emerging biomaterial‐based strategies and underlying mechanisms for modulating endogenous tendon stem/progenitor cells (TSPCs). It offers the most recent insights into TSPC physiology and potential applications of tissue engineering and regenerative medicine in tendons.
Zeyu Zhu, Renwang Sheng, Mumin Cao, Ludvig J. Backman, Yucheng Gao, Hao Wang, Cheng Zhang, Jialin Chen, Wei Zhang, Yunfeng Rui   +9 more
wiley   +1 more source

TRIM28-mediated SUMOylation of SREBF1 drives PD‑L1 N‑glycosylation and immune evasion in bladder cancer. [PDF]

Cell Death Dis
Chen R, Su Y, Chen J, Zhu S, Chen X, Su Y.   +5 more
europepmc   +1 more source

Engineering programmable peptide hydrogels for chronic refractory wounds: A mechanism‐driven framework

BMEMat, EarlyView.
To break the self‐perpetuating pathology of chronic wounds, we propose a mechanism‐driven engineering framework for peptide‐based hydrogels. This review elucidates a hierarchical strategy—from molecular design to intelligent, multi‐functional systems—that enables these programmable biomaterials to dynamically intervene in key pathological loops and ...
Xiaoyu Lv, Xinyi Chao, Jiamin Zhang, Jianfeng Liu   +3 more
wiley   +1 more source