Results 11 to 20 of about 10,567,748 (150)

Structure-Based Demystification of Radical Catalysis by a Coenzyme B₁₂ Dependent Enzyme-Crystallographic Study of Glutamate Mutase with Cofactor Homologues. [PDF]

Angew Chem Int Ed Engl, 2022
Glutamate mutase (GM) catalyses the interconversion of glutamate and 3‐methylaspartate. It binds its B12‐cofactor AdoCbl with an essential misfit for its Ado‐group, liberating the clamped down aggressive Ado‐radical in a tight reaction space to even promote “negative catalysis”. A “simply stretched” AdoCbl‐homolog, in contrast, fits GM, freezing up the
Gruber K, Csitkovits V, Łyskowski A, Kratky C, Kräutler B.   +4 more
europepmc   +4 more sources

Engineered C-N Lyases for Stereoselective Synthesis of Tertiary Amines. [PDF]

Angew Chem Int Ed Engl
EDDS lyase was subjected to iterative cycles of site‐saturation mutagenesis and screened for increased activity for the addition of 2‐((methylamino)methyl)aniline to fumarate. The final mutant, EDDS lyase CEA, accepts various N‐methyl‐1‐phenylmethanamines derivatives, yielding N,N‐disubstituted L‐aspartic acids, as well as several ortho‐substituted ...
Bothof L, Gong X, Onclin ME, Fodran P, Poelarends GJ.   +4 more
europepmc   +3 more sources

Integrated Transcriptomic Analysis Provided Diagnostic and Pathophysiological Insights for Epilepsy. [PDF]

J Immunol Res
Background: Epilepsy is a common neurological disorder involving multiple genes and molecular pathways. Study of differentially expressed genes (DEGs) and hub genes related to epilepsy can help reveal the pathophysiologic basis and improve potential diagnostic and therapeutic strategies.
Li S, Wang Z, Zheng Y, Ma Y, Huang Z, Lian Y.   +5 more
europepmc   +2 more sources

Enzymatic Oxy‐ and Amino‐Functionalization in Biocatalytic Cascade Synthesis: Recent Advances and Future Perspectives

Angewandte Chemie, Volume 135, Issue 48, November 27, 2023., 2023
Oxygenation and amination reactions are widespread in synthetic chemistry to produce valuable compounds. Nowadays, the importance of sustainable strategies to introduce oxygen and amino functionalities into organic molecules is increasing.
Eleonora Grandi, Fatma Feyza Özgen, Sandy Schmidt, Gerrit J. Poelarends   +3 more
wiley   +2 more sources

Creation of Environmentally Friendly Super “Dinitrotoluene Scavenger” Plants

Advanced Science, Volume 10, Issue 30, October 26, 2023., 2023
This study presents a novel approach to developing environmentally friendly “dinitrotoluene scavengers” plants capable of efficiently degrading 2,4‐dinitrotoluene (2,4‐DNT) into harmless compounds while recovering ATP and acetyl‐CoA through the 2,4‐DNT degradative products.
Jian‐Jie Gao, Zhen‐Jun Li, Bo Zhu, Li‐Juan Wang, Jing Xu, Bo Wang, Xiao‐Yan Fu, Hong‐Juan Han, Wen‐Hui Zhang, Yong‐Dong Deng, Yu Wang, Zhi‐Hao Zuo, Ri‐He Peng, Yong‐Sheng Tian, Quan‐Hong Yao   +14 more
wiley   +1 more source

Myeloid vitamin D receptor regulates Paneth cells and microbial homeostasis

The FASEB Journal, Volume 37, Issue 6, June 2023., 2023
Myeloid cells regulate crypt differentiation and the microbiota in a Vitamin D receptor (VDR)‐dependent mechanism. VDRΔLyz mice (without myeloid VDR) exhibited impaired Paneth cell, significant changes in the microbiota, and susceptibility to infection. Loss of myeloid VDR impaired Wnt secretion in macrophages, thus inhibiting crypt β‐catenin signaling
Danika S. Bakke, Jilei Zhang, Yongguo Zhang, Destiny Ogbu, Yinglin Xia, Jun Sun   +5 more
wiley   +1 more source

Maternal obesity alters offspring liver and skeletal muscle metabolism in early post‐puberty despite maintaining a normal post‐weaning dietary lifestyle

The FASEB Journal, Volume 36, Issue 12, December 2022., 2022
Abstract Maternal obesity (MO) during pregnancy is linked to increased and premature risk of age‐related metabolic diseases in the offspring. However, the underlying molecular mechanisms still remain not fully understood. Using a well‐established nonhuman primate model of MO, we analyzed tissue biopsies and plasma samples obtained from post‐pubertal ...
Isaac Ampong, Kip D. Zimmerman, Danu S. Perumalla, Katharyn E. Wallis, Ge Li, Hillary F. Huber, Cun Li, Peter W. Nathanielsz, Laura A. Cox, Michael Olivier   +9 more
wiley   +1 more source

Microfluidic devices for studying bacterial taxis, drug testing and biofilm formation

Microbial Biotechnology, Volume 15, Issue 2, Page 395-414, February 2022., 2022
Microfluidics is an emerging technique that can complements current biological assays. Here, we review some key microfluidic‐based studies describing the effects of different microenvironmental conditions on bacterial phenotypes. We also highlight the potential and possibilities of using microfluidic‐based technology to study bacterial phenotypes in ...
Sandra Pérez‐Rodríguez, José Manuel García‐Aznar, Jesús Gonzalo‐Asensio   +2 more
wiley   +1 more source

EPSPs in rat neocortical neurons in vitro. II. Involvement of N-methyl-D-aspartate receptors in the generation of EPSPs [PDF]

, 1989
1. Intracellular recordings were obtained from neurons in layer II/III of rat frontal cortex. Single-electrode current- and voltage-clamp techniques were employed to compare the sensitivity of excitatory postsynaptic potentials (EPSPs) and ...
J. J. Hablitz, Sutor, Bernd, Hablitz, John H., B. Sutor   +3 more
core   +1 more source

Recent Applications of Carbon‐Nitrogen Lyases in Asymmetric Synthesis of Noncanonical Amino Acids and Heterocyclic Compounds

ChemBioChem, Volume 21, Issue 19, Page 2733-2742, October 1, 2020., 2020
Going backwards to go forwards: The ability of carbon‐nitrogen lyases to work in reverse and connect diverse building blocks through C−N links offers exciting opportunities for biocatalytic retrosynthesis and for the preparation of complex biologically active molecules.
Jielin Zhang, Mohammad Z. Abidin, Thangavelu Saravanan, Gerrit J. Poelarends   +3 more
wiley   +1 more source