A fluorometric method for the assay of protein kinase activity [PDF]
, 2018 Protein kinases constitute one of the largest protein families in nature. Current methods to assay their activity involve the use of radioactive ATP or very expensive reagents.
Figueroa, Carlos Maria +4 more
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Frontiers in Cellular Neuroscience, 2018 Cumulating evidence has indicated NAD+ deficiency as a common central pathological factor of multiple diseases and aging. NAD+ supplement is highly protective in various disease and aging models, while two key questions have remained unanswered: (1) Does
Jie Zhang +5 more
doaj +1 more source
High yield 1,3-propanediol production by rational engineering of the 3-hydroxypropionaldehyde bottleneck in Citrobacter werkmanii [PDF]
, 2016 Background: Imbalance in cofactors causing the accumulation of intermediates in biosynthesis pathways is a frequently occurring problem in metabolic engineering when optimizing a production pathway in a microorganism.
Avcı, Fatma Gizem +5 more
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Activation of Akt by advanced glycation end products (AGEs): involvement of IGF-1 receptor and caveolin-1. [PDF]
PLoS ONE, 2013 Diabetes is characterized by chronic hyperglycemia, which in turn facilitates the formation of advanced glycation end products (AGEs). AGEs activate signaling proteins such as Src, Akt and ERK1/2.
Su-Jung Yang +7 more
doaj +1 more source
Activation of AMP-activated protein kinase by metformin induces protein acetylation in prostate and ovarian cancer cells [PDF]
, 2016 AMP-activated protein kinase (AMPK) is an energy sensor and master regulator of metabolism. AMPK functions as a fuel gauge monitoring systemic and cellular energy status.
Galdieri, Luciano +3 more
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MNADK, a novel liver-enriched mitochondrion-localized NAD kinase
Biology Open, 2013 Summary NADP+ and its reducing equivalent NADPH are essential for counteracting oxidative damage. Mitochondria are the major source of oxidative stress, since the majority of superoxide is generated from the mitochondrial respiratory chain. Because NADP+
Ren Zhang
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Journal of Diabetes Investigation, 2020 One of the biochemical abnormalities found in diabetic tissues is a decrease in the cytosolic oxidized to reduced forms of the nicotinamide adenine dinucleotide ratio (NAD+/NADH also known as pseudohypoxia) caused by oxidation of excessive substrates ...
Lan Fan, Jose M. Cacicedo, Yasuo Ido
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Lapatinib-binding protein kinases in the African trypanosome: identification of cellular targets for kinase-directed chemical scaffolds. [PDF]
, 2013 Human African trypanosomiasis is caused by the eukaryotic microbe Trypanosoma brucei. To discover new drugs against the disease, one may use drugs in the clinic for other indications whose chemical scaffolds can be optimized via a medicinal chemistry ...
Abagyan, Ruben +7 more
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The effector AvrRxo1 phosphorylates NAD in planta. [PDF]
PLoS Pathogens, 2017 Gram-negative bacterial pathogens of plants and animals employ type III secreted effectors to suppress innate immunity. Most characterized effectors work through modification of host proteins or transcriptional regulators, although a few are known to ...
Teja Shidore +7 more
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Increased Adenine Nucleotide Degradation in Skeletal Muscle Atrophy [PDF]
, 2019 Adenine nucleotides (AdNs: ATP, ADP, AMP) are essential biological compounds that facilitate many necessary cellular processes by providing chemical energy, mediating intracellular signaling, and regulating protein metabolism and solubilization.
Brault, Jeffrey J. +2 more
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