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Evidence for NAD Nucleosidase in Rabbit-Liver Lysosomes
Canadian Journal of Biochemistry, 1975A method is described for the isolation of secondary lysosomes from homogenates of rabbit liver. The uptake of Triton WR-1339 by rabbit-liver lysosomes when administered by intraperitoneal injection was used to decrease the density of secondary lysosomes. Lysosomal fractions prepared by this method contain an NAD nucleosidase (NAD glycohydrolase, EC 3.
A, Mellors, A K, Lun, O N, Peled
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Purification and properties of NAD nucleosidase from Fusarium nivale
Biochimica et Biophysica Acta (BBA) - Enzymology, 1973Abstract 1. 1.|A highly active NAD nucleosidase (NAD glycohydrolase, EC 3.2.2.5) is synthesized by the snow mold Fusarium nivale. The activity appears in cell-bound or soluble form depending on the composition of the growth medium and the cultural conditions; formation of the enzyme seems related to the process of sporulation. 2.
D, Stathakos, I, Isaakidou, H, Thomou
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Acta Endocrinologica, 1983
Abstract. In prostatic cytosol DHT1 is metabolized to 5α-androstane-3α (or β), 17α-diols with a half life of 2 h even at 4°C. Thus, [3H]DHT appears to be a poor marker for a quantitative assessment of androgen receptors (AR). Methyltrienolone (R1881) seems to be advantageous as it is not metabolized.
H, Moeller +3 more
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Abstract. In prostatic cytosol DHT1 is metabolized to 5α-androstane-3α (or β), 17α-diols with a half life of 2 h even at 4°C. Thus, [3H]DHT appears to be a poor marker for a quantitative assessment of androgen receptors (AR). Methyltrienolone (R1881) seems to be advantageous as it is not metabolized.
H, Moeller +3 more
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Purification and some properties of NAD-degrading purine nucleosidase from Aspergillus niger
Canadian Journal of Biochemistry, 1978An enzyme which degrades NAD at the adenine–ribose linkage has been purified from the mycelial extract of Aspergillus niger. NADP, deamido-NAD, and purine nucleosides and nucleotides were also susceptible to the hydrolytic cleavage. Pyrimidine- and nicotinamide–ribose linkages were not attacked.
M, Kuwhara, T, Fujii
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Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
Abstract The effectiveness of nicotinamide and 5′-methyl nicotinamide as inhibitors of nuclear NAD nucleosidase and poly ADP-ribose polymerase from rat liver has been investigated. Nicotinamide inhibited the NAD nucleosidase and the poly ADP-ribose polymerase with Ki's of 5 · 10−4 M and 2 · 10−5 M, respectively, 5′-methyl nicotinamide with Ki's of 3 ...
J B, Clark, G M, Ferris, S, Pinder
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Abstract The effectiveness of nicotinamide and 5′-methyl nicotinamide as inhibitors of nuclear NAD nucleosidase and poly ADP-ribose polymerase from rat liver has been investigated. Nicotinamide inhibited the NAD nucleosidase and the poly ADP-ribose polymerase with Ki's of 5 · 10−4 M and 2 · 10−5 M, respectively, 5′-methyl nicotinamide with Ki's of 3 ...
J B, Clark, G M, Ferris, S, Pinder
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Structural and biochemical basis of NAD+ nucleosidase activity of TIR domain-containing proteins
2021NAD+ (nicotinamide adenine dinucleotide) was first identified as a cofactor in many redox reactions. Subsequently, it has been found to synchronize many cellular functions through the regulation of NAD+ consuming enzymes like CD38, sirtuins, and PARPs (poly ADP-ribose polymerases).
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Folia Microbiologica, 1980
Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving primarily as its source.
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Purification of streptococcal extracellular NAD+ nucleosidase is associated with changes of kinetic properties. A high-molecular weight component is required for a full activity of the enzyme. The component is not produced by bacteria and is present in the Todd-Hewitt cultivation medium, the beef-heart extract serving primarily as its source.
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Safety assessment of purine nucleosidase from Aspergillus luchuensis
Toxicology Research and Application, 2021Trung D Võ, Seth Floyd
exaly

