Results 61 to 70 of about 686 (83)

NAD Nucleosidase of Agkistrodon Bilineatus Venom

The American Journal of Tropical Medicine and Hygiene, 1978
NAD nucleosidase (NAD glycohydrolase, EC 3.2.2.5) activity in Agkistrodon bilineatus venom was observed. Using the cyanide assay method at pH 7.9, lyophilized crude venom had an activity of 0.19 units/mg. Chromatography of the crude venom on DEAE Sephadex A-50 with ammonium acetate buffer by two stage elution yielded 13 fractions. Peak NAD nucleosidase
E. M. Brunson   +2 more
openaire   +3 more sources

Evidence for NAD Nucleosidase in Rabbit-Liver Lysosomes

Canadian Journal of Biochemistry, 1975
A method is described for the isolation of secondary lysosomes from homogenates of rabbit liver. The uptake of Triton WR-1339 by rabbit-liver lysosomes when administered by intraperitoneal injection was used to decrease the density of secondary lysosomes. Lysosomal fractions prepared by this method contain an NAD nucleosidase (NAD glycohydrolase, EC 3.
O. N. Peled, A. K. L. Lun, A. Mellors
openaire   +3 more sources

Purification and properties of NAD nucleosidase from Fusarium nivale

Biochimica et Biophysica Acta (BBA) - Enzymology, 1973
Abstract 1. 1.|A highly active NAD nucleosidase (NAD glycohydrolase, EC 3.2.2.5) is synthesized by the snow mold Fusarium nivale. The activity appears in cell-bound or soluble form depending on the composition of the growth medium and the cultural conditions; formation of the enzyme seems related to the process of sporulation. 2.
Dimitri Stathakos   +2 more
openaire   +3 more sources

Purification and some properties of NAD-degrading purine nucleosidase from Aspergillus niger

Canadian Journal of Biochemistry, 1978
An enzyme which degrades NAD at the adenine–ribose linkage has been purified from the mycelial extract of Aspergillus niger. NADP, deamido-NAD, and purine nucleosides and nucleotides were also susceptible to the hydrolytic cleavage. Pyrimidine- and nicotinamide–ribose linkages were not attacked.
M Kuwhara, T Fujii
openaire   +3 more sources

A quantitative assay for the cytoplasmic androgen receptor using [3H]dihydrotestosterone in the presence of NAD+-nucleosidase

Acta Endocrinologica, 1983
Abstract. In prostatic cytosol DHT1 is metabolized to 5α-androstane-3α (or β), 17α-diols with a half life of 2 h even at 4°C. Thus, [3H]DHT appears to be a poor marker for a quantitative assessment of androgen receptors (AR). Methyltrienolone (R1881) seems to be advantageous as it is not metabolized.
Günter Oettling   +3 more
openaire   +3 more sources

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