Results 71 to 80 of about 33,309 (204)

A Photoenzymatic NADH regeneration system [PDF]

, 2018
A photoenzymatic NADH regeneration system was established. The combination of deazariboflavin as a photocatalyst with putidaredoxin reductase enabled the selective reduction of NAD+ into the enzyme‐active 1,4‐NADH to promote an ...
Choi, Eun-Gyu, Eun‐Gyu Choi, Frank Hollmann, Elena Fernández‐Fueyo, Pesic, M. (author), Urlacher, Vlada B. (author), Hollmann, F. (author), Arends, I.W.C.E. (author), Fernandes-Fueyo, Elena, Hollmann, Frank, Yong H. Kim, Höfler, G.T. (author), Fernandez Fueyo, E. (author), Kim, Yong H. (author), Isabel W. C. E. Arends, Choi, Eun Gyu (author), Younes, Sabry H., Pesic, Milja, Arends, Isabel W. C. E., Younes, S.H.H. (author), Urlacher, Vlada B., Vlada B. Urlacher, Georg T. Höfler, Milja Pesic, Kim, Yong Hwan, Hçfler, Georg T., Sabry H. Younes   +26 more
core   +1 more source

Design of a Novel Diamine Biosensor to Guide the Engineering of Escherichia coli for Cadaverine Overproduction

Advanced Science, EarlyView.
A high‐throughput screening platform was established by integrating an in vivo cadaverine biosensor with a fluorescence‐activated cell sorting (FACS) system. Biosensor‐driven specific exporter tuning, pathway optimization and genome‐wide target mining were conducted to maximize cadaverine production in Escherichia coli.
Jia Feng, Getao Liu, Ding Ma, Jiaxiang Yuan, Xinyi Zhang, Weihao Wang, Chen Ma, Yang Liao, Siyuan Gao, Lei Qin, Jiao Feng, Sheng Xu, Xin Wang, Kequan Chen   +13 more
wiley   +1 more source

Near Infrared‐Mediated Intracellular NADH Delivery Strengthens Mitochondrial Function and Stability in Muscle Dysfunction Model

Advanced Science
Mitochondrial transfer emerges as a promising therapy for the restoration of mitochondrial function in damaged cells, mainly due to its limited immunogenicity.
Hui Bang Cho, Hye‐Ryoung Kim, Sujeong Lee, Chae Won Cho, Ji‐In Park, Seulki Youn, Gyuwon So, Sumin Kang, Hye Jin Kim, Keun‐Hong Park   +9 more
doaj   +1 more source

Nicotinamide pre-treatment ameliorates NAD(H) hyperoxidation and improves neuronal function after severe hypoxia

Neurobiology of Disease, 2014
Prolonged hypoxia leads to irreversible loss of neuronal function and metabolic impairment of nicotinamide adenine dinucleotide recycling (between NAD+ and NADH) immediately after reoxygenation, resulting in NADH hyperoxidation.
Pavan K. Shetty, Francesca Galeffi, Dennis A. Turner   +2 more
doaj   +1 more source

The Beta Subunit of Non-bifurcating NADH-Dependent [FeFe]-Hydrogenases Differs From Those of Multimeric Electron-Bifurcating [FeFe]-Hydrogenases

Frontiers in Microbiology, 2020
A non-bifurcating NADH-dependent, dimeric [FeFe]-hydrogenase (HydAB) from Syntrophus aciditrophicus was heterologously produced in Escherichia coli, purified and characterized.
Nathaniel A. Losey, Saroj Poudel, Eric S. Boyd, Michael J. McInerney   +3 more
doaj   +1 more source

An improved method for the determination of NADH oxidase in the presence of NADH peroxidase in lactic acid bacteria

, 2003
The complexity of the coupled NADH oxidase–NADH peroxidase enzyme system in lactic acid bacteria makes it difficult to simultaneously determine the individual levels of both these enzymes spectrophotometrically.
Talwalkar, Akshat, Hourigan, Jim, Peiris, Paul, Kailasapathy, Kasipathy, Arumugaswamy, Ramakrishnaswamy   +4 more
core   +1 more source

ROS/pH‐Responsive Phenylboronic Acid–Chitosan Nanoparticles Improve Silymarin‐Mediated Hepatoprotection After Hepatectomy With Gut‐Liver Axis‐Associated Responses

Advanced Science, EarlyView.
Bioactive phenylboronic acid–chitosan nanoparticles enable responsive silymarin delivery for post‐hepatectomy liver protection. The nanoplatform integrates antioxidant and antibacterial functions, improves hepatic recovery, and is associated with coordinated shifts in gut microbiota, metabolites, cytokines, and hepatic proteomic pathways, suggesting a ...
Jinjin Tong, Chaochao Luo, Zezheng Liu, Xinyan Zhang, Daixu Jia, Yang Sun, Zhaonan Zhang, Haoran Shi, Jianfang Wang, Hua Zhang   +9 more
wiley   +1 more source

Mechanistic Insights on the Magnesium(II) Ion-Activated Reduction of Methyl Benzoylformate with Chelated NADH Peptide β-Lactam Models

, 2009
Mechanistic details of the Mg(2+) ion-activated enantioselective reduction of methyl benzoylformate have been investigated at a B3LYP/6-31G* theory level, using peptide NADH models 1 rigidified with a beta-lactam ring. Computation of the reaction pathway
Palomo, Claudio, Santos, José I., Fratila, Raluca M., Ferrón, Pablo, Benito, Ana M., Gómez-Bengoa, Enrique, Aizpurua, Jesús M., Miranda, José Ignacio, José I. Miranda (1397344), Pablo Ferrón (2352169), José I. Santos (2352172), Jesus M. Aizpurua (1397347), Claudio Palomo (1699948), Ana Benito (1817008), Raluca M. Fratila (673916), Enrique Gómez-Bengoa (1508548), Jesus M. Aizpurua, Claudio Palomo, Enrique Gómez-Bengoa, Raluca M. Fratila, Ana Benito, Pablo Ferrón, José I. Santos, José I. Miranda   +23 more
core   +1 more source

Bioenergetic Materials for Tissue Regeneration: Modulating Metabolism to Promote Cellular Anabolism

Advanced Science, EarlyView.
Bioenergetic materials (BEMs) modulate cellular metabolism to promote tissue regeneration. Their principal mechanisms of action include metabolic substrate supplementation, metabolic enzyme modulation, organelle transfer, electrical current regulation, redox homeostasis modulation, and oxygen tension modulation.
Yuchen He, Yuwen Wang, Jonathan F. Gong, Yiting Lei, Fei Jin, Weihong Zhu, Rocky S. Tuan, Zhong Alan Li   +7 more
wiley   +1 more source

Physiological relevance, localization and substrate specificity of the alternative (type II) mitochondrial NADH dehydrogenases of Ogataea parapolymorpha

Frontiers in Microbiology
Mitochondria from Ogataea parapolymorpha harbor a branched electron-transport chain containing a proton-pumping Complex I NADH dehydrogenase and three Type II NADH dehydrogenases (NDH-2).
Hannes Juergens, Álvaro Mielgo-Gómez, Albert Godoy-Hernández, Jolanda ter Horst, Janine M. Nijenhuis, Duncan G. G. McMillan, Duncan G. G. McMillan, Robert Mans   +7 more
doaj   +1 more source