Results 281 to 290 of about 125,071 (335)
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Apollo-NADP+: a spectrally tunable family of genetically encoded sensors for NADP+

Nature Methods, 2016
NADPH-dependent antioxidant pathways have a critical role in scavenging hydrogen peroxide (H2O2) produced by oxidative phosphorylation. Inadequate scavenging results in H2O2 accumulation and can cause disease. To measure NADPH/NADP(+) redox states, we explored genetically encoded sensors based on steady-state fluorescence anisotropy due to FRET ...
William D, Cameron   +5 more
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Photoreduction of NAD and NADP by chlorophyllin a in the presence of ascorbate: Requirement for NADP reductase

Archives of Biochemistry and Biophysics, 1965
Abstract Chlorophyllin a catalyzes the photoreduction of NADP (and NAD to a lesser extent) in the presence of ascorbate as electron donor and NADP reductase as the coupling enzyme. Photosynthetic pyridine nucleotide reductase is inactive in the reaction and does not stimulate the reaction observed with NADP reductase.
L P, VERNON, A S, PIETRO, D A, LIMBACH
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Ferredoxin : NADP+ Oxidoreductase

2018
Ferredoxin-NADP + reductase, the essential catalyst of NADP + photoreduction, is a flavoprotein ubiquitous in photosynthetic eukaryotic cells and Cyano-bacteria. The cyanobacteria enzyme has a much lower content of methionine and lysine and more histidines.
G. Zanetti, A. Allverti
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The Synthesis of Poly-L-Lysine-Succinyl-Nadp: An Analogue for Nadp/H

The International Journal of Artificial Organs, 1980
Poly-L-lysine-succinyl-NADP, an analogue for nicotinamide adenine dinucleotide phosphate (NADP/H), has been synthesized by linking NADP/H to poly-L-lysine hydrobromide. This analogue (PL-SNP) was assayed with N, N dimethylaniline (DMA) in the presence of hepatic microsomal oxidase. A regeneration system of G-6-p and G-6-PD was used for reducing the PL-
R A, Wills, W M, Southard, S S, Sofer
openaire   +2 more sources

How is ferredoxin-NADP reductase involved in the NADP photoreduction of chloroplasts?

Photosynthesis Research, 2004
NADP photoreduction of chloroplasts was discovered in 1951, and subsequent research was conducted to elucidate the enzymatic mechanisms involved in this reaction. In 1963, ferredoxin-NADP reductase (FNR; EC 1.18.1.2, ferredoxin-NADP oxidoreductase) was isolated and purified to a crystalline form.
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A lysyl residue at the NADP binding site of ferredoxin-NADP reductase

Biochimica et Biophysica Acta (BBA) - Enzymology, 1976
Dansyl chloride, at low molar ratio, inactivates ferredoxin-NADP reductase (NADPH:ferredoxin oxidoreductase, EC 1.6.7.1). The complete protection afforded either by NADP or NADPH suggests a direct involvement of the active site. Experiments with [Me-14C] dansyl chloride showed that about 1.5 residues per flavin were dansylated: by differential ...
openaire   +2 more sources

Enzymatic Synthesis of Caged NADP Cofactors:  Aqueous NADP Photorelease and Optical Properties

The Journal of Organic Chemistry, 2000
The synthesis of caged NADP analogues 18, 19, and 20 has been accomplished by utilizing the transglycosidase activity of solubilized NAD glycohydrolase (porcine brain) to incorporate caged nicotinamides 2, 3, and 4 into NADP. The synthesis of several nicotinamides modified at the carboxamide with o-nitrobenzyl photolabile groups is demonstrated as well
C P, Salerno, D, Magde, A P, Patron
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Conversations with NADP

Trends in Microbiology, 1998
Catabolite repression is a central multigene regulatory network that enables bacteria to select and utilize the optimal carbon and energy sources available in a given environment. Cyclic AMP and catabolite gene activator protein (CAP) play central roles in mediating catabolite repression in enteric bacteria but are absent from Gram-positive bacteria ...
openaire   +1 more source

Complex-forming properties of butanedione-modified ferredoxin-NADP+ reductase with NADP+ and ferredoxin

Archives of Biochemistry and Biophysics, 1979
Abstract The plastidic ferredoxin-NADP + reductase from the xanthophycean alga Bumilleriopsis forms a stoichiometric 1:1 complex with ferredoxin and NADP + which is demonstrated by difference spectra of both complexes. Butanedione modification of the flavoprotein results in loss of its enzymatic activities (transhydrogenase and diaphorase ...
G, Bookjans, P, Böger
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Analogues of NADP+ as inhibitors and coenzymes for NADP+ malic enzyme from maize leaves

Photosynthesis Research, 1991
Structural analogues of the NADP(+) were studied as potential coenzymes and inhibitors for NADP(+) dependent malic enzyme from Zea mays L. leaves. Results showed that 1, N(6)-etheno-nicotinamide adenine dinucleotide phosphate (∈ NADP(+)), 3-acetylpyridine-adenine dinucleotide phosphate (APADP(+)), nicotinamide-hypoxanthine dinucleotide phosphate (NHDP(+
C P, Spampinato   +3 more
openaire   +2 more sources

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