Results 201 to 210 of about 15,085 (211)

[Enzymatic activities of recombinant human NAMPT and NAMPT (H247A) proteins].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences, 2011
To prepare and purify recombinant human NAMPT and NAMPT (H247A) proteins and to detect their enzymatic activity.Using pcDNA3.1-hnampt as template, full-length hnampt was sub-cloned into pET-11a(+) plasmid. The hnampt (H247A) mutant was obtained by site-directed mutagenesis.
Feng, Wang, Peng, Huang, Zhu, Liu, Yun-Bi, Lu, Er-Qing, Wei, Wei-Ping, Zhang, Chun, Tang   +6 more
openaire   +1 more source

Abstract 4024: NAMPT inhibitor decreases NAMPT capture by an antibody directed against the 5-phosphoribosyl-1-pyrophosphate-binding loop: Rationale for an NAMPT occupancy assay

Cancer Research, 2020
Abstract Nicotinamide phosphoribosyl transferase (NAMPT) catalyzes the rate-limiting step of nicotinamide adenine dinucleotide (NAD) biosynthesis. As NAMPT inhibitors (NAMPTi) have been shown to be substrates of NAMPT, binding of NAMPTi to NAMPT results in the NAMPTi-phosphoribose adduct (RP) that binds tightly and strongly inhibits the ...
Maysoun Shomali, Sandrine Hilairet, Marianne Duhamel, Frederico Nardi, Thomas Bertrand, Geraldine Penarier, Laurent Debussche, Monsif Bouaboula   +7 more
openaire   +1 more source

Identification of novel resistance mechanisms to NAMPT inhibition via the de novo NAD+ biosynthesis pathway and NAMPT mutation

Biochemical and Biophysical Research Communications, 2017
Cancer cells have an unusually high requirement for the central and intermediary metabolite nicotinamide adenine dinucleotide (NAD+), and NAD+ depletion ultimately results in cell death. The rate limiting step within the NAD+ salvage pathway required for converting nicotinamide to NAD+ is catalyzed by nicotinamide phosphoribosyltransferase (NAMPT ...
Jun, Guo, Lloyd T, Lam, Kenton L, Longenecker, Mai H, Bui, Kenneth B, Idler, Keith B, Glaser, Julie L, Wilsbacher, Chris, Tse, William N, Pappano, Tzu-Hsuan, Huang   +9 more
openaire   +2 more sources

Fragment-based discovery of a potent NAMPT inhibitor

Bioorganic & Medicinal Chemistry Letters, 2018
NAMPT expression is elevated in many cancers, making this protein a potential target for anticancer therapy. We have carried out both NMR based and TR-FRET based fragment screens against human NAMPT and identified six novel binders with a range of potencies. Co-crystal structures were obtained for two of the fragments bound to NAMPT while for the other
Alla Korepanova, Kenton L. Longenecker, Steve D. Pratt, Sanjay C. Panchal, Richard F. Clark, Marc Lake, Sujatha M. Gopalakrishnan, Diana Raich, Chaohong Sun, Andrew M. Petros   +9 more
openaire   +2 more sources

Nicotinamide phosphoribosyltransferase (Nampt) in carcinogenesis: new clinical opportunities

Expert Review of Anticancer Therapy, 2016
Nicotinamide phosphoribosyltransferase (Nampt) is the rate-limiting enzyme that catalyzes the first step in the mammalian nicotinamide adenine dinucleotide (NAD) salvage pathway. Aberrant NAD metabolism was associated with oncogenic signal transduction, suggesting the critical roles of Nampt in tumorigenesis and metastasis.
Hang, Chen, Shiyu, Wang, Haiyuan, Zhang, Edouard C, Nice, Canhua, Huang   +4 more
openaire   +2 more sources

[Mechanism of Nampt gene expression regulation].

Yi chuan = Hereditas, 2013
Nicotinamide phosphoribosyltransferase (NAMPT), also known as pre-B cell colony-enhancing factor (PBEF) or visfatin, is a crucial rate-limiting enzyme of NAD biosynthetic pathway. It may affect the metabolism, inflammatory response, cell proliferation, differentiation, and apoptosis, especially the aging and other physiological progresses through ...
Rui, Huang, Zi-Ru, Wang, Yu, Sun, Gang, Zhang, Ya-Ping, Lu   +4 more
openaire   +1 more source

An NAMPT Inhibitor Decreases NAMPT Capture by an Antibody Directed against the 5-Phosphoribosyl-1-Pyrophosphate-Binding Loop: A Rational for an NAMPT Occupancy Assay

AbstractNicotinamide phosphoribosyltransferase (NAMPT) catalyzes the rate-limiting step of nicotinamide adenine dinucleotide (NAD) biosynthesis. NAMPT inhibitors (NAMPTi) have been shown to be NAMPT substrates. The resulting NAMPTi-phosphoribose (RP) adduct binds tightly to NAMPT and inhibits the enzyme. Using new NAMPTi, SAR154782, structural analyses
Maysoun Shomali, Sandrine Hilairet, Marianne Duhamel, Frederico Nardi, Thomas Bertrand, Magali Mathieu, Rosalia Arrebola, Geraldine Penarier, Samir Jegham, Jerome Arigon, Sylvie Cosnier, Monsif Bouaboula   +11 more
openaire   +1 more source

NAMPT neuroprotection

Science-Business eXchange, 2014
openaire   +1 more source

Visfatin/NAMPT – a hot spot in thrombosis?

Thrombosis Research, 2012
Bente, Halvorsen, Tuva B, Dahl, Pål, Aukrust   +2 more
openaire   +2 more sources

Nampt deletion blinds mice

Nature Reviews Endocrinology, 2016
openaire   +1 more source