Results 1 to 10 of about 300,505 (163)
The Mitochondrial NAD Transporter SLC25A51 in Adipocytes Regulates Adipose Tissue Mitochondrial Function and Systemic Metabolism During Aging. [PDF]
Aging CellWe revealed the pathophysiological significance and therapeutic potential of adipocyte mitochondrial NAD transporter, namely solute carrier family 25 member 51 (SLC25A51), in age‐associated metabolic diseases, particularly adipose tissue mitochondrial function, obesity, and insulin resistance, by analyzing adipocyte‐specific Slc25a51 knockout (ASKO ...
Kojima D +19 more
europepmc +2 more sources
“NAD-capQ” detection and quantitation of NAD caps [PDF]
RNA, 2018 RNA 5′ cap structures comprising the metabolic effector nicotinamide adenine dinucleotide (NAD) have been identified in diverse organisms. Here we report a simple, two-step procedure to detect and quantitate NAD-capped RNA, termed “NAD-capQ.” By use of NAD-capQ we quantitate NAD-capped RNA levels in Escherichia coli, Saccharomyces cerevisiae, and human
Grudzien-Nogalska, Ewa +3 more
openaire +2 more sources
Nicotinamide adenine dinucleotide (NAD+). Formal potential of the NAD+/NAD· couple and NAD· dimerization rate [PDF]
Biochimica et Biophysica Acta (BBA) - Bioenergetics, 1984 The rate constant, kd, for the dimerization of the free radical (NAD), produced on the initial one-electron reduction of NAD+, was measured by double potential-step chronoamperometry, fast-scan cyclic voltammetry (cathodic-anodic peak current ratio) and slow-scan cyclic voltammetry (peak potential shift) for a medium in which neither NAD+ nor its ...
M A, Jensen, P J, Elving
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Assays for NAD+-Dependent Reactions and NAD+ Metabolites [PDF]
, 2018 Nicotinamide adenine dinucleotide (NAD+) is an essential redox cofactor and signaling molecule that controls the activity of enzymes involved in metabolism, DNA repair, and cellular survival, such as the PARPs, CD38, and the sirtuins. Here, we describe three methods for measuring the activity of these enzymes: the etheno-NAD+ assay measures NAD ...
Michael B, Schultz +3 more
openaire +2 more sources
Aging Cell, Volume 21, Issue 7, July 2022., 2022 Nicotinamide riboside (NR) and caffeine increase nicotinamide adenine dinucleotide (NAD), transiently modulate bioenergetic functions, and change the expression of NAD‐synthesizing, recycling, or degrading genes in dermal fibroblasts or induced pluripotent stem cell (iPSC)‐derived neural progenitors and astrocytes from late‐onset Alzheimer's disease ...
Woo‐In Ryu +6 more
wiley +1 more source
CD38 Drives Progress of Osteoarthritis by Affecting Cartilage Homeostasis
Orthopaedic Surgery, Volume 14, Issue 5, Page 946-954, May 2022., 2022 CD38 is a membrane‐bound protein. This study primarily implicates CD38 as an important regulator of chondrogenic differentiation. Inhibition of CD38 demonstrated protection against cartilage degeneration, which suggests that CD38 could be a potential therapeutic target for OA.
Jin‐jin Ma +9 more
wiley +1 more source
CD38 inhibitor 78c increases mice lifespan and healthspan in a model of chronological aging
Aging Cell, Volume 21, Issue 4, April 2022., 2022 The NADase CD38 plays a key role in age‐related NAD decline. The CD38 inhibitor 78c increases lifespan and healthspan of naturally aged male mice. The increase in median and maximal lifespan was about 10%. Abstract Nicotinamide adenine dinucleotide (NAD) levels decline during aging, contributing to physical and metabolic dysfunction.
Thais R. Peclat +11 more
wiley +1 more source
Acta Crystallographica Section F, Volume 78, Issue 2, Page 52-58, February 2022., 2022 The crystal structure of a short‐chain dehydrogenase/reductase from Burkholderia phymatum offers insights into its possible functions and likely inhibitors of its enzymatic functions.Burkholderia phymatum is an important symbiotic nitrogen‐fixing betaproteobacterium. B.
Jawaher Alenazi +4 more
wiley +1 more source
The FEBS Journal, Volume 289, Issue 4, Page 955-964, February 2022., 2022 Increased IDO activity towards de novo nicotinamide synthesis is a hallmark of alternatively activated macrophages. IDO upregulation in alternatively activated macrophages may be an attempt to compensate for insufficient NAD availability. Manipulation of nicotinamide metabolism may shift macrophage metabolism, thereby providing a therapeutic approach ...
Melinda S. Suchard, Dana M. Savulescu
wiley +1 more source
Acta Crystallographica Section D, Volume 77, Issue 10, Page 1317-1335, October 2021., 2021 The homospermidine synthases from P. aeruginosa and B. viridis, as well as their single‐residue variants, are compared based on crystal structures and activity assays. A high structural similarity is demonstrated, suggesting the equivalent involvement of relevant residues in the reaction mechanism and catalytic dependence on cation–π interaction ...
F. Helfrich, Axel J. Scheidig
wiley +1 more source