Results 211 to 220 of about 62,172 (259)
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In‐depth performance investigation of a nano‐LC gradient generator
ELECTROPHORESIS, 2012AbstractAn innovative approach for nano‐liquid chromatography (LC) gradient generation is presented. This system represents an optimized and refined version of a prototype proposed by the authors a few years ago: the current version is characterized by a new configuration that guarantees complete automation and easier operation.
A. Siviero +5 more
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Prospects for monolithic nano-LC columns in shotgun proteomics
Analytical and Bioanalytical Chemistry, 2008We report a premier side-by-side comparison of two leading types of monolithic nano-LC column (silica-C(18), polystyrene) in shotgun proteomics experiments. Besides comparing the columns in terms of the number of peptides from a real-life sample (Arabidopsis thaliana chloroplast) that they identified, we compared the monoliths in terms of peak capacity
Vilém, Guryca +3 more
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Biospecific interaction (affinity) CEC and affinity nano‐LC
ELECTROPHORESIS, 2007AbstractThis review article is aimed at assessing the recent progress made in affinity nano‐LC and affinity CEC performed in capillaries and microchips. A variety of biospecific interactions is covered including lectin affinity, immunoaffinity, immobilized metal affinity, sugar‐based affinity, protein A affinity, protein G affinity, aptamer affinity ...
Fred M, Okanda, Ziad, El Rassi
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Preventing Carryover of Peptides and Proteins in Nano LC-MS Separations
Analytical Chemistry, 2009Sample carryover is a significant problem that occurs in high-performance liquid chromatography (HPLC) analysis. Carryover effects cannot be tolerated in any high-performance liquid chromatography-mass spectroscopy (HPLC-MS) separation system, and proteomics analysis must be performed in a separation system with virtually no carryover.
Goran, Mitulović +6 more
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Application of Nano-LC-Based Glycomics Towards Biomarker Discovery
Bioanalysis, 2011The glycome, that is, the glycan components of a biological source, has been widely reported to change with disease states. However, mining the glycome for biomarkers is complicated by glycan structural heterogeneity. Nanoflow LC, or nano-LC, significantly addresses the problem by providing a highly sensitive and quantitative method of separating and ...
Serenus, Hua +2 more
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Nano-LC-MS/MS for the quantitation of prostanoids in immune cells
Analytical and Bioanalytical Chemistry, 2014Prostanoids, derivatives of arachidonic acid, are involved in inflammation and immune reactions. To understand the role of prostanoids produced by diverse immune cells, a highly sensitive quantitation method for prostaglandin E2 (PGE2), prostaglandin D2 (PGD2), 6-keto prostaglandin F1α (6-keto PGF1α), prostaglandin F2α (PGF2α), and thromboxane B2 (TXB2)
Thomas +8 more
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Enantiomeric separations by means of nano‐
Enantiomers represent a class of compounds extensively investigated since they can show totally different behaviors when they interact with a chiral environment. Because of their identical chemical structure (they differ only in the spatial arrangement of the atoms in the molecule), the separation of optical isomers is a challenging task of analytical ...
A Rocco, A Maruska, S Fanali
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Analysis of Aloe‐based phytotherapeutic products by using nano‐LC‐MS
Journal of Separation Science, 2010AbstractThis article proposes a chromatographic method for the analysis of extracts of Aloe plants. The method was developed with a laboratory assembled nano‐LC system coupled with a UV detector, followed by an IT‐mass spectrometer. With a step gradient mode of ACN/H2O mixtures and employing a capillary column packed with C18 (100 μm id), a complete ...
S Fanali +6 more
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Use of novel phenyl‐hexyl core‐shell particles in nano‐LC
ELECTROPHORESIS, 2013This paper reports the use of novel phenyl‐hexyl core‐shell particles packed into fused silica capillaries in nano‐LC. Capillary columns of different id of 25, 50, 75, 100, and 150 μm were packed employing the slurry packing method. The columns were used for the separation of a model mixture containing five aromatic hydrocarbons.
Salvatore, Fanali +2 more
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A method for quantification of serum tenascin-X by nano-LC/MS/MS
Clinica Chimica Acta, 2016Complete deficiency of an extracellular matrix tenascin-X (TNX) leads to a classical type of Ehlers-Danlos syndrome (EDS). TNX haploinsufficiency is a cause of hypermobility type of EDS. Human TNX is also present in a serum form (sTNX) with a molecular size of 140kDa.
Kazuo, Yamada +3 more
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