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Nano-LC and HPLC-Chip–ESI–MS: An Emerging Technique for Glycobioanalysis
Bioanalysis, 2009In less than 5 years, an impressive number of methods based on nano-LC and HPLC-chip coupled online to MS were developed and implemented to comprehensively address structural heterogeneity of glycoconjugates and glycans in biological matrices. C18, graphitized carbon and amide-based stationary phases were adapted to nanoflow level and on chip format ...
Stephan, Kirsch, Laura, Bindila
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Nano LC–MS/MS: A Robust Setup for Proteomic Analysis
2011Nanoscale liquid chromatography coupled to tandem mass spectrometry (nano LC-MS/MS) has become an essential tool in the field of proteomics. In fact, its sensitivity has advantages over conventional LC-MS/MS that allow the analysis of peptide mixtures in sample-limited situations (e.g., proteolytically digested proteins isolated by two-dimensional gel ...
Marco, Gaspari, Giovanni, Cuda
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Capillary-liquid chromatography (CLC) and nano-LC in food analysis
TrAC Trends in Analytical Chemistry, 2013Abstract Capillary-liquid chromatography (CLC) and nano-LC are miniaturized techniques with great potential in the analytical field. They have been applied in different areas – pharmaceuticals, biomedicine, environment and recently food and agrochemicals.
C. Fanali +3 more
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Monitoring of post-mortem changes of saliva N-glycosylation by nano LC/MS
Analytical and Bioanalytical Chemistry, 2017The estimation of post-mortem interval (PMI) is a crucial part for investigations of crime and untimely deaths in forensic science. However, standard methods of PMI estimation are easily confounded by extenuating circumstances and/or environmental factors.
Bum Jin, Kim +8 more
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Polar Anionic Metabolome Analysis by Nano-LC/MS with a Metal Chelating Agent
Analytical Chemistry, 2009We have developed a practical method for the comprehensive analysis of polar anionic metabolites in biological samples with the use of a nano-LC/MS system. A polyamine-bonded polymer-based apHera NH2 column, which is compatible with ammonium carbonate buffer, effectively retained anionic polar metabolites, such as organic acids, sulfates, and ...
Khin Than, Myint +3 more
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Heart‐cut nano‐LC–CZE–MS for the characterization of proteins on the intact level
ELECTROPHORESIS, 2019AbstractMultidimensional separation techniques play an increasingly important role in separation science, especially for the analysis of complex samples such as proteins. The combination of reversed‐phase liquid chromatography in the nanoscale and CZE is especially beneficial due to their nearly orthogonal separation mechanism and well‐suited ...
Kevin Jooß +3 more
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Analysis of Complex Protein Mixtures Using Nano-LC Coupled to MS/MS
Cold Spring Harbor Protocols, 2006INTRODUCTIONNanoliter-LC coupled to tandem mass spectrometry (nano-LC-MS/MS) permits the rapid and sensitive determination of protein-protein interactions. By using a specific purification technique such as coimmunoprecipitation or affinity purification in conjunction with nano-LC-MS/MS, not only are proteins identified, but specific protein-protein ...
David M, Schieltz +2 more
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Methacrylate ester‐based monolithic columns for nano‐LC separation of tocopherols in vegetable oils
Journal of Separation Science, 2010AbstractThe separation and determination of tocopherols (Ts) in vegetable oils by nano‐LC chromatography with UV–vis detection using lauryl methacrylate ester‐based monolithic columns has been developed. The separation of Ts was optimized in terms of mobile phase composition on the basis of the best compromise among efficiency, resolution and analysis ...
M. J. Lerma Garcıa +4 more
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ELECTROPHORESIS, 2019
AbstractThis work deals with the potentiality of nano liquid chromatography (Nano‐LC) for the chiral separation of racemic mixture of tryptophan and some selected derivatives by using 100 µm i.d. fused silica capillary packed with teicoplanin bonded to 5 µm diol silica stationary phase.
Giovanni D'Orazio +3 more
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AbstractThis work deals with the potentiality of nano liquid chromatography (Nano‐LC) for the chiral separation of racemic mixture of tryptophan and some selected derivatives by using 100 µm i.d. fused silica capillary packed with teicoplanin bonded to 5 µm diol silica stationary phase.
Giovanni D'Orazio +3 more
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Nano-LC: introduction and recent applications
2007-
SFanali, Z Aturki, G D'Orazio, A Rocco
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