Results 81 to 90 of about 1,581 (100)

Nano-Differential Scanning Fluorimetry for Screening in Fragment-based Lead Discovery

Journal of Visualized Experiments, 2021
Thermal shift assays (TSAs) examine how the melting temperature (Tm) of a target protein changes in response to changes in its environment (e.g., buffer composition). The utility of TSA, and specifically of nano-Differential Scanning Fluorimetry (nano-DSF), has been established over the years, both for finding conditions that help stabilize a specific ...
Ahmad, Misbha Ud Din, Fish, Alexander, Molenaar, Jeroen, Sreeramulu, Sridhar, Richter, Christian, Altincekic, Nadide, Schwalbe, Harald, Wienk, Hans, Perrakis, Anastassis   +8 more
openaire   +2 more sources

An application of Nano Differential Scanning Fluorimetry for Higher Order Structure assessment between mAb originator and biosimilars: Trastuzumab and Rituximab as case studies

Journal of Pharmaceutical and Biomedical Analysis, 2020
Differential scanning fluorimetry (DSF) or thermal shift has emerged in recent years as a high-throughput screening method in biotherapeutic formulation studies. The present article reports on a fast-track assessment platform for rapid investigation of therapeutic proteins such as monoclonal antibodies (mAb) with minimal sample concentration, volume ...
Srishti, Joshi, Chinmoyee, Maharana, Anurag S, Rathore   +2 more
openaire   +2 more sources

Nano Differential Scanning Fluorimetry-Based Thermal Stability Screening and Optimal Buffer Selection for Immunoglobulin G

Pharmaceuticals, 2022
Dong Hee Na, Han Ju Yoo, Soohyun Kim
exaly  

The Use of Differential Scanning Fluorimetry to Assess Strigolactone Receptor Function

Methods in Molecular Biology, 2021
Cyril Hamiaux, Kimberley Snowden, Bart Janssen   +2 more
exaly  

Application of temperature-responsive HIS-tag fluorophores to differential scanning fluorimetry screening of small molecule libraries

Frontiers in Pharmacology, 2022
Michael Ronzetti, Bolormaa Baljinnyam, Ganesha Rai   +2 more
exaly  

Nano-differential scanning fluorimetry as a tool for the assessment of refolded antibody fragments: A case study for anti-Pfs25 single-chain antibodies

Biochemical Engineering Journal
Harvinder Kour Khera, Ashwathi Valiyaparambil, Deepak K. Jagannath, Vysakh K. Viswanath, Naveen Kumar, Jay Prakash Shukla, Sabyasachi Pradhan, Anirudha Lakshminarasimhan   +7 more
openaire   +1 more source

Intrinsic Differential Scanning Fluorimetry for Fast and Easy Identification of Adeno-Associated Virus Serotypes

Journal of Pharmaceutical Sciences, 2020
Stylianos Michalakis, Magalie Penaud-Budloo, Martin Biel   +2 more
exaly  

Use of differential scanning fluorimetry to optimize the purification and crystallization of PLP-dependent enzymes

Acta Crystallographica Section F: Structural Biology Communications, 2012
Barry C Finzel
exaly  

Meltdown: A Tool to Help in the Interpretation of Thermal Melt Curves Acquired by Differential Scanning Fluorimetry

SLAS Discovery, 2015
David R Lovell, Nicholas Rosa, Janet Newman   +2 more
exaly  

SimpleDSFviewer: A tool to analyze and view differential scanning fluorimetry data for characterizing protein thermal stability and interactions

Protein Science, 2020
Changye Sun, Edwin Yates, Yong Li
exaly