Results 131 to 140 of about 2,893 (161)

Nanoluciferase-Based Complementation Assay to Detect GPCR-G Protein Interaction

Methods in Molecular Biology, 2021
G protein-coupled receptors (GPCR) are one of the principal class of membrane proteins and around 30% of the currently marketed drugs act on one of them. The efficacious detection of ligands with the desired pharmacological profile remains a challenge of paramount importance in the GPCR drug discovery and pharmacological research.
Julien Hanson, Celine Laschet
exaly   +3 more sources

Nanoluciferase Reporter Mycobacteriophage for Sensitive and Rapid Detection of Mycobacterium tuberculosis Drug Susceptibility

Journal of Bacteriology, 2020
Mycobacterium tuberculosis , the causative agent of tuberculosis disease, remains a public health crisis on a global scale, and development of new interventions and identification of drug resistance are pillars in the World Health Organization End TB Strategy.
Michael Berney, Chelsea Steiner, Diane Ordway   +2 more
exaly   +3 more sources

Protein A–Nanoluciferase fusion protein for generalized, sensitive detection of immunoglobulin G

Analytical Biochemistry, 2023
Detection and quantification of antibodies, especially immunoglobulin G (IgG), is a cornerstone of ELISAs, many diagnostics, and the development of antibody-based drugs. Current state-of-the-art immunoassay techniques for antibody detection require species-specific secondary antibodies and carefully-controlled bioconjugations.
Suman Nandy, Mary Crum, Katherine Wasden, Ulrich Strych, Atul Goyal, Vijay Maranholkar, William Mo, Binh Vu, Katerina Kourentzi, Richard C. Willson   +9 more
openaire   +2 more sources

Use of Reverse Genetics for the Generation of Recombinant Influenza Viruses Carrying Nanoluciferase

2023
Influenza A (FLUAV) and influenza B (FLUBV) viruses are human and/or animal pathogens widely studied due to their importance to public health and animal production. Both FLUAV and FLUBV possess a genome composed of eight viral gene segments. For reverse genetics of influenza viruses, transcription of the mRNA for the viral proteins is typically done ...
C Joaquin, Caceres, L Claire, Gay, Flavio Cargnin, Faccin, Daniel R, Pérez   +3 more
openaire   +2 more sources

Nanoluciferase-based complementation assay for systematic profiling of GPCR–GRK interactions

2022
G protein-coupled receptor kinases (GRKs) are a family of seven soluble receptor-modifying enzymes which are essential regulators of GPCR activity. Following agonist-induced receptor activation and G protein dissociation, GRKs prime the receptor for desensitization through phosphorylation of its C terminus, which subsequently allows arrestins to bind ...
Palmer, Christie B., D'Uonnolo, Giulia, Luís, Rafael, Meyrath, Max, Uchański, Tomasz, Chevigné, Andy, Szpakowska, Martyna   +6 more
openaire   +2 more sources

A versatile nanoluciferase toolkit and optimized in-gel detection method for protein analysis in plants

Molecular Breeding, 2021
Dissection of gene function requires sophisticated tools to monitor gene expression. Gene tagging with epitope peptides and fluorescent protein tags is a routine method to investigate protein expression using tag-specific antibodies and western blotting with tedious blotting and immunodetection steps.
Hong Li, Caiyun Wu, Manman Du, Yache Chen, Xin Hou, Yinong Yang, Kabin Xie   +6 more
openaire   +2 more sources

Nanoluciferase-based methods to monitor activation, modulation and trafficking of atypical chemokine receptors

2022
Chemokines regulate directed cell migration, proliferation and survival and are key components in various physiological and pathological processes. They exert their functions by interacting with seven-transmembrane domain receptors that signal through G proteins (GPCRs).
Luís, Rafael, D’Uonnolo, Giulia, Palmer, Christie B., Meyrath, Max, Uchański, Tomasz, Wantz, May, Rogister, Bernard, Janji, Bassam, Chevigné, Andy, Szpakowska, Martyna   +9 more
openaire   +2 more sources

A nanoluciferase SFTSV for rapid screening antivirals and real-time visualization of virus infection in mice

EBioMedicine
Severe fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne pathogen that causes severe hemorrhagic fever in humans, but no FDA-approved specific antivirals or vaccines are available to treat or prevent SFTS.The plasmids construction and transfection were performed to generate the recombinant SFTSV harboring the nanoluciferase ...
Junyuan Cao
exaly   +3 more sources

Novel furimazine derivatives for nanoluciferase bioluminescence with various C-6 and C-8 substituents

Organic & Biomolecular Chemistry, 2021
Nanoluciferase (NLuc) is the emerging commercially available luciferase considering its small size and superior bioluminescence performance.
Jie Li, Xiaoxu Wang, Gaopan Dong, Chongzheng Yan, Yuanyuan Cui, Zheng Zhang, Lupei Du, Minyong Li   +7 more
openaire   +2 more sources

A novel ultrasensitive bioluminescent receptor-binding assay of INSL3 through chemical conjugation with nanoluciferase

Biochimie, 2013
Insulin-like peptide 3 (INSL3) is a reproduction-related peptide hormone belonging to the insulin/relaxin superfamily, which mediates testicular descent in the male fetus, suppresses male germ cell apoptosis and promotes oocyte maturation in adults by activating the relaxin family peptide receptor 2 (RXFP2).
Zhan-Yun Guo, Ya-Li Liu, Xiao-Xia Shao   +2 more
exaly   +3 more sources