Results 151 to 160 of about 3,590 (177)

Anticodon-edited transfer RNAs (ACE-tRNAs) encoded as therapeutic nonviral minimal DNA vectors. [PDF]

Nucleic Acids Res
Porter JJ, Ko W, Sorensen EG, Cheung Z, Thrasher K, Couch T, Gabell JT, Shwe V, Hyatt J, Licata JB, Peterson LK, Dean DA, Lueck JD.   +12 more
europepmc   +1 more source

Mapping the dynamic plant interactome: from in vitro assays to in vivo quantitative approaches. [PDF]

Plant Methods
Hussain MA, Hafeez AH, Noor I, Shakoor A, Hussain H, Gholizadeh F, Sohail H.   +6 more
europepmc   +1 more source

NanoBRET-based detection of ligand-receptor interactions at the neuropeptide FF receptor 1. [PDF]

RSC Adv
Lentschat H, Beck-Sickinger AG.
europepmc   +1 more source

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Protein A–Nanoluciferase fusion protein for generalized, sensitive detection of immunoglobulin G

Analytical Biochemistry, 2023
Detection and quantification of antibodies, especially immunoglobulin G (IgG), is a cornerstone of ELISAs, many diagnostics, and the development of antibody-based drugs. Current state-of-the-art immunoassay techniques for antibody detection require species-specific secondary antibodies and carefully-controlled bioconjugations.
Suman Nandy, Mary Crum, Katherine Wasden, Ulrich Strych, Atul Goyal, Vijay Maranholkar, William Mo, Binh Vu, Katerina Kourentzi, Richard C. Willson   +9 more
openaire   +2 more sources

Nanoluciferase Reporter Mycobacteriophage for Sensitive and Rapid Detection of Mycobacterium tuberculosis Drug Susceptibility.

Journal of Bacteriology, 2020
Mycobacterium tuberculosis , the causative agent of tuberculosis disease, remains a public health crisis on a global scale, and development of new interventions and identification of drug resistance are pillars in the World Health Organization End TB Strategy.
Paras Jain, Michael Berney, Chelsea Steiner   +2 more
exaly   +3 more sources

Use of Reverse Genetics for the Generation of Recombinant Influenza Viruses Carrying Nanoluciferase

2023
Influenza A (FLUAV) and influenza B (FLUBV) viruses are human and/or animal pathogens widely studied due to their importance to public health and animal production. Both FLUAV and FLUBV possess a genome composed of eight viral gene segments. For reverse genetics of influenza viruses, transcription of the mRNA for the viral proteins is typically done ...
C Joaquin, Caceres, L Claire, Gay, Flavio Cargnin, Faccin, Daniel R, Pérez   +3 more
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Nanoluciferase-based complementation assay for systematic profiling of GPCR–GRK interactions

2022
G protein-coupled receptor kinases (GRKs) are a family of seven soluble receptor-modifying enzymes which are essential regulators of GPCR activity. Following agonist-induced receptor activation and G protein dissociation, GRKs prime the receptor for desensitization through phosphorylation of its C terminus, which subsequently allows arrestins to bind ...
Palmer, Christie B., D'Uonnolo, Giulia, Luís, Rafael, Meyrath, Max, Uchański, Tomasz, Chevigné, Andy, Szpakowska, Martyna   +6 more
openaire   +2 more sources

A versatile nanoluciferase toolkit and optimized in-gel detection method for protein analysis in plants

Molecular Breeding, 2021
Dissection of gene function requires sophisticated tools to monitor gene expression. Gene tagging with epitope peptides and fluorescent protein tags is a routine method to investigate protein expression using tag-specific antibodies and western blotting with tedious blotting and immunodetection steps.
Hong Li, Caiyun Wu, Manman Du, Yache Chen, Xin Hou, Yinong Yang, Kabin Xie   +6 more
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Nanoluciferase-based methods to monitor activation, modulation and trafficking of atypical chemokine receptors

2022
Chemokines regulate directed cell migration, proliferation and survival and are key components in various physiological and pathological processes. They exert their functions by interacting with seven-transmembrane domain receptors that signal through G proteins (GPCRs).
Luís, Rafael, D’Uonnolo, Giulia, Palmer, Christie B., Meyrath, Max, Uchański, Tomasz, Wantz, May, Rogister, Bernard, Janji, Bassam, Chevigné, Andy, Szpakowska, Martyna   +9 more
openaire   +2 more sources