Results 161 to 170 of about 3,590 (177)
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Organic & Biomolecular Chemistry, 2021
Nanoluciferase (NLuc) is the emerging commercially available luciferase considering its small size and superior bioluminescence performance.
Jie Li +7 more
openaire +2 more sources
Nanoluciferase (NLuc) is the emerging commercially available luciferase considering its small size and superior bioluminescence performance.
Jie Li +7 more
openaire +2 more sources
Biochimie, 2013
Insulin-like peptide 3 (INSL3) is a reproduction-related peptide hormone belonging to the insulin/relaxin superfamily, which mediates testicular descent in the male fetus, suppresses male germ cell apoptosis and promotes oocyte maturation in adults by activating the relaxin family peptide receptor 2 (RXFP2).
Zhan-Yun Guo +2 more
exaly +3 more sources
Insulin-like peptide 3 (INSL3) is a reproduction-related peptide hormone belonging to the insulin/relaxin superfamily, which mediates testicular descent in the male fetus, suppresses male germ cell apoptosis and promotes oocyte maturation in adults by activating the relaxin family peptide receptor 2 (RXFP2).
Zhan-Yun Guo +2 more
exaly +3 more sources
EBioMedicineSevere fever with thrombocytopenia syndrome virus (SFTSV) is an emerging tick-borne pathogen that causes severe hemorrhagic fever in humans, but no FDA-approved specific antivirals or vaccines are available to treat or prevent SFTS.The plasmids construction and transfection were performed to generate the recombinant SFTSV harboring the nanoluciferase ...
Junyuan Cao, Yuxi Wen
exaly +3 more sources
Biosensors and BioelectronicsIn this work, with parathion, a typical forbidden organophosphate pesticide as target drug, an enhanced nanobody-driven bioluminescent immunoassay based on the engineered split-nanoluciferase (NanoLuc) was proposed. Concretely, through labeling 11S and β10, two split-NanoLuc units onto the anti-parathion nanobody (Nb) VHH9 and the artificial antigen H1
Hiroshi Ueda, Hong Wang, Yudong Shen
exaly +4 more sources
Biochemical Pharmacology
Hypoxia-inducible factors (HIF) are interesting targets for multiple therapeutic indications. While HIF activation is desired for the treatment of anemia-related and ischemic diseases, HIF inhibition is of tremendous interest to anti-cancer drug development. Different signaling events within the HIF pathway are being targeted by drug discovery programs,
Liesl K. Janssens, Christophe P. Stove
openaire +2 more sources
Hypoxia-inducible factors (HIF) are interesting targets for multiple therapeutic indications. While HIF activation is desired for the treatment of anemia-related and ischemic diseases, HIF inhibition is of tremendous interest to anti-cancer drug development. Different signaling events within the HIF pathway are being targeted by drug discovery programs,
Liesl K. Janssens, Christophe P. Stove
openaire +2 more sources
2022
PARP7 is an enzyme that catalyzes mono-ADP-ribosylation (MARylation), is a critical regulator of type I interferon signaling, and has emerged as an immune-oncology drug candidate. PARP7 is a labile protein that is regulated in a proteasome-dependent manner. Indeed, endogenous PARP7 levels are undetectable by western blot in most cells.
Anna K, Duell +2 more
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PARP7 is an enzyme that catalyzes mono-ADP-ribosylation (MARylation), is a critical regulator of type I interferon signaling, and has emerged as an immune-oncology drug candidate. PARP7 is a labile protein that is regulated in a proteasome-dependent manner. Indeed, endogenous PARP7 levels are undetectable by western blot in most cells.
Anna K, Duell +2 more
openaire +2 more sources
Biosensors and Bioelectronics
Here, we developed nanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction (NanaPPI). When an interaction occurs, primary antibodies for two proteins bring the proximity of secondary nanobody-fused small/large fragment to reassemble into an intact NanoLuc variant ...
Qianqian Li +7 more
openaire +2 more sources
Here, we developed nanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction (NanaPPI). When an interaction occurs, primary antibodies for two proteins bring the proximity of secondary nanobody-fused small/large fragment to reassemble into an intact NanoLuc variant ...
Qianqian Li +7 more
openaire +2 more sources
Molecular Pharmacology
ATP-binding cassette (ABC) transporters expressed at the blood-brain barrier (BBB) impede delivery of therapeutic agents to the brain, including agents to treat neurodegenerative diseases and primary and metastatic brain cancers. Two transporters, ABCB1 and ABCG2, are highly expressed at the BBB and are responsible for the efflux of numerous clinically
John A. Quinlan +9 more
openaire +2 more sources
ATP-binding cassette (ABC) transporters expressed at the blood-brain barrier (BBB) impede delivery of therapeutic agents to the brain, including agents to treat neurodegenerative diseases and primary and metastatic brain cancers. Two transporters, ABCB1 and ABCG2, are highly expressed at the BBB and are responsible for the efflux of numerous clinically
John A. Quinlan +9 more
openaire +2 more sources
Endogenous Promotor-Driven Split Nanoluciferase Biosensor for Assessing G Protein Recruitment
Abstract HEK293 cells are a common immortal cell line used in biological research, and their popularity has led to different distinct lineages across the world. Commonly used for overexpression of proteins, HEK293 cells also natively express biological targets, such as G protein coupled receptors (GPCRs) and their ...Laura J. Humphrys +2 more
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Nanoluciferase-Based Reporters for Clostridioides difficile
Jeroen Corver +2 moreopenaire +1 more source