Results 141 to 150 of about 2,893 (161)
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Biochemical Pharmacology
Hypoxia-inducible factors (HIF) are interesting targets for multiple therapeutic indications. While HIF activation is desired for the treatment of anemia-related and ischemic diseases, HIF inhibition is of tremendous interest to anti-cancer drug development. Different signaling events within the HIF pathway are being targeted by drug discovery programs,
Liesl K. Janssens, Christophe P. Stove
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Hypoxia-inducible factors (HIF) are interesting targets for multiple therapeutic indications. While HIF activation is desired for the treatment of anemia-related and ischemic diseases, HIF inhibition is of tremendous interest to anti-cancer drug development. Different signaling events within the HIF pathway are being targeted by drug discovery programs,
Liesl K. Janssens, Christophe P. Stove
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2021
Here we describe the stepwise application of bioluminescence resonance energy transfer (BRET)-based conformational receptor biosensors to study GPCR activation in intact cells. This technology can be easily adopted to various plate reader devices and microtiter plate formats. Due to the high sensitivity of these BRET-based receptor biosensors and their
Hannes, Schihada +3 more
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Here we describe the stepwise application of bioluminescence resonance energy transfer (BRET)-based conformational receptor biosensors to study GPCR activation in intact cells. This technology can be easily adopted to various plate reader devices and microtiter plate formats. Due to the high sensitivity of these BRET-based receptor biosensors and their
Hannes, Schihada +3 more
openaire +2 more sources
2023
Receptor tyrosine kinases (RTKs) are transmembrane receptors activated by a wide diversity of growth factors, cytokines or hormones. They ensure multiple roles in cellular processes, including proliferation, differentiation and survival. They are also crucial drivers of development and progression of multiple cancer types, and represent important drug ...
Dosquet, Hugo +7 more
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Receptor tyrosine kinases (RTKs) are transmembrane receptors activated by a wide diversity of growth factors, cytokines or hormones. They ensure multiple roles in cellular processes, including proliferation, differentiation and survival. They are also crucial drivers of development and progression of multiple cancer types, and represent important drug ...
Dosquet, Hugo +7 more
openaire +4 more sources
Process Biochemistry, 2015
Abstract Escherichia coli is widely used to produce recombinant proteins. For soluble overexpression, fusion technology has been developed using different fusion partners. In the present work, we used a newly developed nanoluciferase (NanoLuc) as a novel fusion partner for soluble overexpression of the aggregation-prone interleukin-6 (IL6). Soluble 6×
Ben-Jun Ji +3 more
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Abstract Escherichia coli is widely used to produce recombinant proteins. For soluble overexpression, fusion technology has been developed using different fusion partners. In the present work, we used a newly developed nanoluciferase (NanoLuc) as a novel fusion partner for soluble overexpression of the aggregation-prone interleukin-6 (IL6). Soluble 6×
Ben-Jun Ji +3 more
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Biosensors and Bioelectronics
Here, we developed nanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction (NanaPPI). When an interaction occurs, primary antibodies for two proteins bring the proximity of secondary nanobody-fused small/large fragment to reassemble into an intact NanoLuc variant ...
Qianqian Li +7 more
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Here, we developed nanobody-assisted nanoluciferase fragment complementation for in situ measurement and visualization of endogenous protein-protein interaction (NanaPPI). When an interaction occurs, primary antibodies for two proteins bring the proximity of secondary nanobody-fused small/large fragment to reassemble into an intact NanoLuc variant ...
Qianqian Li +7 more
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Molecular Pharmacology
ATP-binding cassette (ABC) transporters expressed at the blood-brain barrier (BBB) impede delivery of therapeutic agents to the brain, including agents to treat neurodegenerative diseases and primary and metastatic brain cancers. Two transporters, ABCB1 and ABCG2, are highly expressed at the BBB and are responsible for the efflux of numerous clinically
John A. Quinlan +9 more
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ATP-binding cassette (ABC) transporters expressed at the blood-brain barrier (BBB) impede delivery of therapeutic agents to the brain, including agents to treat neurodegenerative diseases and primary and metastatic brain cancers. Two transporters, ABCB1 and ABCG2, are highly expressed at the BBB and are responsible for the efflux of numerous clinically
John A. Quinlan +9 more
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Endogenous Promotor-Driven Split Nanoluciferase Biosensor for Assessing G Protein Recruitment
Abstract HEK293 cells are a common immortal cell line used in biological research, and their popularity has led to different distinct lineages across the world. Commonly used for overexpression of proteins, HEK293 cells also natively express biological targets, such as G protein coupled receptors (GPCRs) and their ...Laura J. Humphrys +2 more
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Nanoluciferase-Based Reporters for Clostridioides difficile
Jeroen Corver +2 moreopenaire +1 more source