Results 71 to 80 of about 101,026 (352)

Nanopore-Based Single-Entity Electrochemistry for the Label-Free Monitoring of Single-Molecule Glycoprotein–Boronate Affinity Interaction and Its Sensing Application

, 2022
Nanopipettes provide a promising confined space that enables advances in single-molecule analysis, and their unique conical tubular structure is also suitable for single-cell analysis.
Mengya Cao (8728635), Mengya Cao, Haoran Tang (6274499), Dandan Zhao, Yanyan Zhu (32035), Yongxin Li, Haoran Tang, Yanyan Zhu, Dandan Zhao (582723), Hao Wang, Hao Wang (39217), Yongxin Li (471129)   +11 more
core   +1 more source

Low‐Cost, Large‐Scale Nanoporous Metals by Mechanical Alloying, Oxide Reduction, and Dealloying of Powders

Advanced Engineering Materials, EarlyView.
Powder metal processing provides scalable advantages in nanoporous (np) metal development. Mechanical alloying is used to produce unique precursors for hybrid nanopore formation by oxide reduction and dealloying. As demonstrated in np Ag, this approach improves process efficiency while promoting smaller ligaments and larger pores, both of which are ...
Mark A. Atwater, Oliver A. Fowler
wiley   +1 more source

Nanopore native RNA sequencing of a human poly(A) transcriptome

Nature Methods, 2018
High-throughput complementary DNA sequencing technologies have advanced our understanding of transcriptome complexity and regulation. However, these methods lose information contained in biological RNA because the copied reads are often short and ...
R. Workman, Alison D. Tang, Paul S. Tang, Miten Jain, J. R. Tyson, Roham Razaghi, P. Zuzarte, Timothy Gilpatrick, Alexander Payne, J. Quick, Norah Sadowski, N. Holmes, Jaqueline Goes de Jesus, K. Jones, C. Soulette, T. Snutch, N. Loman, Benedict Paten, M. Loose, J. Simpson, Hugh E. Olsen, Angela N. Brooks, M. Akeson, W. Timp   +23 more
semanticscholar   +1 more source

Planar Solid‐State Nanopores Toward Scalable Nanofluidic Integration Based on CMOS Technology

Advanced Engineering Materials, EarlyView.
We present a scalable silicon‐based fabrication strategy for planar solid‐state nanopores to enable their integration with complex nanofluidic systems. Prototype devices demonstrate normal voltage‐current characteristics, good noise performance, and appreciable streaming currents. Our CMOS‐compatible fabrication process offers precise geometric control
Ngan Hoang Pham, Chenyu Wen, Mohammad Hadi Khaksaran, Won‐Yong Lee, Javier Cruz, Klas Hjort, Dongping Wu, Shi‐Li Zhang   +7 more
wiley   +1 more source

Recent progress in the methods of genome sequencing

Brazilian Archives of Biology and Technology, 2010
Genome sequencing is a very important tool for the development of genetic diagnosis, drugs of gene engineering, pharmacogenetics, etc. As the HGP comes into people's ears, there is an emerging need for the genome sequencing.
Zhao Ning-wei
doaj   +1 more source

Next- and Third-Generation Sequencing Outperforms Culture-Based Methods in the Diagnosis of Ascitic Fluid Bacterial Infections of ICU Patients

Cells, 2021
Objectives: Infections of the ascitic fluid are serious conditions that require rapid diagnosis and treatment. Ascites is often accompanied by other critical pathologies such as gastrointestinal bleeding and bowel perforation, and infection increases the
Hanna Goelz, Simon Wetzel, Negin Mehrbarzin, Stefan Utzolino, Georg Häcker, Mohamed Tarek Badr   +5 more
doaj   +1 more source

Pattern Recognition of microRNA Expression in Body Fluids using Nanopore Decoding at Sub-femtomolar Concentration

, 2021
This paper describes nanopore decoding for microRNA (miRNA) expression patterns using DNA computing technology. miRNAs have shown promise as markers for cancer diagnosis due to their cancer type-specificity, and therefore simple strategies for miRNA ...
Ryuji, Kawano, Nanami, Takeuchi, Moe, Hiratani   +2 more
core   +1 more source

Incorporation of Selenium into Sol–Gel‐Derived Bioactive Glass: Influence on Glass Structure, Bioactivity, and its Selective Cytotoxicity

Advanced Engineering Materials, EarlyView.
Selenium was incorporated into a sol–gel‐derived bioactive glass to enable sustained therapeutic ion release. The selenium‐containing glass preserved bioactivity while selectively inducing cytotoxicity in osteosarcoma cells and maintaining osteoblastic viability.
Breno Rocha Barrioni, Thalita Marcolan Valverde, Talita Martins, Guilherme Mattos Jardim Costa, Alfredo Miranda de Goes, Marcello Rosa Dumont, Eduardo Henrique Martins Nunes, Marivalda de Magalhães Pereira   +7 more
wiley   +1 more source

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome

Nature Communications
Abundant cellular transcripts occupy most of the sequencing reads in the transcriptome, making it challenging to assay for low-abundant transcripts. Here, we utilize the adaptive sampling function of Oxford Nanopore sequencing to selectively deplete and ...
Jiaxu Wang, Lin Yang, Anthony Cheng, Cheng-Yong Tham, Wenting Tan, Jefferson Darmawan, Paola Florez de Sessions, Yue Wan   +7 more
doaj   +1 more source

Homopolish: a method for the removal of systematic errors in nanopore sequencing by homologous polishing

Genome Biology, 2021
Nanopore sequencing has been widely used for the reconstruction of microbial genomes. Owing to higher error rates, errors on the genome are corrected via neural networks trained by Nanopore reads. However, the systematic errors usually remain uncorrected.
Yao-Ting Huang, Po‐Yu Liu, Pei-Wen Shih   +2 more
semanticscholar   +1 more source