Results 61 to 70 of about 70,205 (303)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Microbial Characteristics and Genomic Analysis of an ST11 Carbapenem-Resistant Klebsiella pneumoniae Strain Carrying blaKPC−2 Conjugative Drug-Resistant Plasmid

Frontiers in Public Health, 2022
BackgroundThe sequence type 11 (ST11) carbapenem-resistant Klebsiella pneumoniae (CRKP) carrying blaKPC−2 has been widespread all over the world, and it has been reported frequently in China.
Lingyi Zeng, Lingyi Zeng, Jisheng Zhang, Kewang Hu, Kewang Hu, Jie Li, Jianmin Wang, Chengru Yang, Chengru Yang, Wan Huang, Lining Yin, Lining Yin, Xiaoli Zhang   +12 more
doaj   +1 more source

Nanopore-Based Single-Entity Electrochemistry for the Label-Free Monitoring of Single-Molecule Glycoprotein–Boronate Affinity Interaction and Its Sensing Application

, 2022
Nanopipettes provide a promising confined space that enables advances in single-molecule analysis, and their unique conical tubular structure is also suitable for single-cell analysis.
Mengya Cao (8728635), Mengya Cao, Haoran Tang (6274499), Dandan Zhao, Yanyan Zhu (32035), Yongxin Li, Haoran Tang, Yanyan Zhu, Dandan Zhao (582723), Hao Wang, Hao Wang (39217), Yongxin Li (471129)   +11 more
core   +1 more source

Compound Heterozygote Friedreich Ataxia Patients With Covert Proximal FXN Gene Deletions

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT We present Friedreich ataxia patients with frataxin gene deletions. Data and records were collected at the Children's Hospital of Philadelphia from patients enrolled in the FACOMS natural history study. Patients with proximal deletions initially diagnosed with only one GAA expanded allele had more severe disease than their homozygous expansion
Michael P. Lazaropoulos, Morgan C. Devore, Christina Lam, Courtney Park, Sanjay Bidichandani, David R. Lynch   +5 more
wiley   +1 more source

Hybrid genome assembly and phenotypic assays reveal carbohydrate metabolism diversity in Lacticaseibacillus strains

Applied Microbiology and Biotechnology
Investigation of carbohydrate metabolism in lactic acid bacteria is essential for the rational selection of strains for fermentation processes, particularly in emerging applications involving non-conventional substrates or building of synthetic microbial
Emanuele Della Monica, Worarat Kruasuwan, Natnicha Wankaew, Tantip Arigul, Thidathip Wongsurawat, Camilla Lazzi, Monica Gatti, Alessia Levante   +7 more
doaj   +1 more source

Stability and bandwidth investigation of alternative structures for nanopore sensors [PDF]

, 2010
The genetic information carriers, DNA molecules can be thought of as the blueprints of living organisms. This crucial functionality of the DNA mole- cules may explain the drive and momentum for DNA sequencing research.
Yemenicioglu, Sukru
core  

Augmenting and Assaying Nav1.1 Protein Quantity for Dravet Syndrome Therapy

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Dravet Syndrome (DS) is a developmental and epileptic encephalopathy predominantly caused by heterozygous loss‐of‐function variants in SCN1A, which encodes Nav1.1. Conserved upstream open reading frames (uORFs) in SCN1A were validated to regulate translation in reporter assays, demonstrating the therapeutic viability of increasing Nav1.1 from ...
Aiswarya Saravanan, Jordan Safran, Anna Loughman, Grace Lin, Shivanshi Vaid, Dana Allababidi, Peter M. Tessier, Louis T. Dang   +7 more
wiley   +1 more source

Direct RNA sequencing coupled with adaptive sampling enriches RNAs of interest in the transcriptome

Nature Communications
Abundant cellular transcripts occupy most of the sequencing reads in the transcriptome, making it challenging to assay for low-abundant transcripts. Here, we utilize the adaptive sampling function of Oxford Nanopore sequencing to selectively deplete and ...
Jiaxu Wang, Lin Yang, Anthony Cheng, Cheng-Yong Tham, Wenting Tan, Jefferson Darmawan, Paola Florez de Sessions, Yue Wan   +7 more
doaj   +1 more source

Recent progress in the methods of genome sequencing

Brazilian Archives of Biology and Technology, 2010
Genome sequencing is a very important tool for the development of genetic diagnosis, drugs of gene engineering, pharmacogenetics, etc. As the HGP comes into people's ears, there is an emerging need for the genome sequencing.
Zhao Ning-wei
doaj   +1 more source

Next- and Third-Generation Sequencing Outperforms Culture-Based Methods in the Diagnosis of Ascitic Fluid Bacterial Infections of ICU Patients

Cells, 2021
Objectives: Infections of the ascitic fluid are serious conditions that require rapid diagnosis and treatment. Ascites is often accompanied by other critical pathologies such as gastrointestinal bleeding and bowel perforation, and infection increases the
Hanna Goelz, Simon Wetzel, Negin Mehrbarzin, Stefan Utzolino, Georg Häcker, Mohamed Tarek Badr   +5 more
doaj   +1 more source