Results 41 to 50 of about 101,026 (352)

Nanopore-Based Proteolytic Reactor for Sensitive and Comprehensive Proteomic Analyses

, 2016
Various silica-based microreactors have been designed that use enzyme immobilization to address technical concerns in proteolysis including inefficient and incomplete protein digestion.
Jie Fan (258204), Chunli Liu (459579), Pengyuan Yang (135243), Xian Chen (107385), Jianjun Zhai (3209979), Jie Lei (579923), Dongyuan Zhao (1286025), Yan (3208569), Wenqing Shui (1274865)   +8 more
core   +2 more sources

Identification of tagged glycans with a protein nanopore

Nature Communications, 2023
The structural complexity of glycans seriously challenges the currently available analytical methods. Here, the authors report the identification of glycan isomers, glycans with varying chain lengths, and distinct branched glycans, via a glycan ...
Minmin Li, Yuting Xiong, Yuchen Cao, Chen Zhang, Yuting Li, Hanwen Ning, Fan Liu, Han Zhou, Xiaonong Li, Xianlong Ye, Y. Pang, Jiaming Zhang, Xinmiao Liang, Guangyan Qing   +13 more
semanticscholar   +1 more source

Modeling of single-particle translocation through a low-aspect-ratio nanopore

Journal of Biomechanical Science and Engineering, 2019
A theoretical model for the motion of tiny spherical particles in a nanopore device is developed. The nanopore has a low aspect ratio, and the particles have a radius slightly smaller than that of the nanopore.
Tetsuro TSUJI, Kentaro DOI, Satoyuki KAWANO   +2 more
doaj   +1 more source

Extraction of Organic Compounds From Lacustrine Oil-Prone Shales and the Effect on Nanopore

Frontiers in Earth Science, 2022
Lacustrine oil-prone shale at the oil-generation stage develops a large number of nanopores; however, the influence of fluid–shale interactions on the nanopores of lacustrine shale is poorly understood.
Huairen Cao, Huairen Cao, Deping Zhang, Xiaoyu Wang, Xiaoyu Wang, Deliang Fu   +5 more
doaj   +1 more source

Nanopore direct RNA sequencing maps the complexity of Arabidopsis mRNA processing and m6A modification

eLife, 2020
Understanding genome organization and gene regulation requires insight into RNA transcription, processing and modification. We adapted nanopore direct RNA sequencing to examine RNA from a wild-type accession of the model plant Arabidopsis thaliana and a ...
Matthew T. Parker, Katarzyna Knop, Anna V. Sherwood, N. Schurch, Katarzyna Mackinnon, P. Gould, Anthony J W Hall, G. Barton, G. Simpson   +8 more
semanticscholar   +1 more source

High Prevalence of blaCTXM–1/IncI1-Iγ/ST3 Plasmids in Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolates Collected From Domestic Animals in Guadeloupe (French West Indies)

Frontiers in Microbiology, 2022
Extended-spectrum β-lactamase-producing Enterobacteriaceae (ESBL-E) have been classified in the group of resistant bacteria of highest priority. We determined the prevalence of ESBL-E collected in feces from household and shelter pets in Guadeloupe ...
Gaëlle Gruel, David Couvin, Stéphanie Guyomard-Rabenirina, Guillaume Arlet, Jean-Christophe Bambou, Matthieu Pot, Xavier Roy, Antoine Talarmin, Benoit Tressieres, Séverine Ferdinand, Sébastien Breurec, Sébastien Breurec, Sébastien Breurec   +12 more
doaj   +1 more source

Nanopore-only assemblies

, 2018
These files contain the best nanopore-only assembly produced by ABruijn (pre-corrected + Nanopolish), Canu (Racon x2 + Nanopolish), Flye (pre-corrected + Nanopolish), Miniasm + Minimap (filtered to 100x coverage with Filtlong, Racon x5 + Nanopolish) and ...
Natalie Ring (5116013)
core   +1 more source

Fast nanopore sequencing data analysis with SLOW5

Nature Biotechnology, 2022
Nanopore sequencing depends on the FAST5 file format, which does not allow efficient parallel analysis. Here we introduce SLOW5, an alternative format engineered for efficient parallelization and acceleration of nanopore data analysis.
H. Gamaarachchi, Hiruna Samarakoon, Sasha P. Jenner, James M. Ferguson, T. Amos, J. Hammond, Hassaan Saadat, Martin A. Smith, S. Parameswaran, I. Deveson   +9 more
semanticscholar   +1 more source

Detecting DNA cytosine methylation using nanopore sequencing

Nature Methods, 2017
In nanopore sequencing devices, electrolytic current signals are sensitive to base modifications, such as 5-methylcytosine (5-mC). Here we quantified the strength of this effect for the Oxford Nanopore Technologies MinION sequencer.
Jared T. Simpson, Jared T. Simpson, R. Workman, P. Zuzarte, Matei David, L. Dursi, W. Timp   +6 more
semanticscholar   +1 more source

MinION Nanopore Sequencing Enables Correlation between Resistome Phenotype and Genotype of Coliform Bacteria in Municipal Sewage

Frontiers in Microbiology, 2017
Wastewater treatment plants (WWTPs) functioned as the intersection between the human society and nature environment, are receiving increasingly more attention on risk assessment of the acquisition of environmental antibiotic resistance genes (ARGs) by ...
Yu Xia, An-Dong Li, Yu Deng, Xiao-Tao Jiang, Li-Guan Li, Tong Zhang   +5 more
doaj   +1 more source