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An Introduction to Nanopore Sequencing: Past, Present, and Future Considerations
Micromachines, 2023 There has been significant progress made in the field of nanopore biosensor development and sequencing applications, which address previous limitations that restricted widespread nanopore use.
Morgan Mackenzie, C. Argyropoulos
semanticscholar +1 more source
Performance of neural network basecalling tools for Oxford Nanopore sequencing
Genome Biology, 2019 Basecalling, the computational process of translating raw electrical signal to nucleotide sequence, is of critical importance to the sequencing platforms produced by Oxford Nanopore Technologies (ONT).
R. Wick, L. Judd, K. Holt
semanticscholar +1 more source
Nanopore sequencing and the Shasta toolkit enable efficient de novo assembly of eleven human genomes
Nature Biotechnology, 2020 De novo assembly of a human genome using nanopore long-read sequences has been reported, but it used more than 150,000 CPU hours and weeks of wall-clock time.
Kishwar Shafin +31 more
semanticscholar +1 more source
Microorganisms, 2023 Nanopore sequencing is a promising technology used for 16S rRNA gene amplicon sequencing as it can provide full-length 16S reads and has a low up-front cost that allows research groups to set up their own sequencing workflows.
Márton Szoboszlay +5 more
semanticscholar +1 more source
Advances in nanopore direct RNA sequencing
Nature Methods, 2022 Nanopore direct RNA sequencing (DRS) reads continuous native RNA strands. Early adopters have used this technology to document nucleotide modifications and 3′ polyadenosine tails on RNA strands without added chemistry steps. Individual strands ranging in
Miten Jain +3 more
semanticscholar +1 more source
The evolution of nanopore sequencing [PDF]
Frontiers in Genetics, 2015 The "$1000 Genome" project has been drawing increasing attention since its launch a decade ago. Nanopore sequencing, the third-generation, is believed to be one of the most promising sequencing technologies to reach four gold standards set for the "$1000 Genome" while the second-generation sequencing technologies are bringing about a revolution in life
Wang, Yue, Yang, Qiuping, Wang, Zhimin
openaire +3 more sources
Nanopore sequencing and assembly of a human genome with ultra-long reads
Nature Biotechnology, 2017 We report the sequencing and assembly of a reference genome for the human GM12878 Utah/Ceph cell line using the MinION (Oxford Nanopore Technologies) nanopore sequencer.
Miten Jain +25 more
semanticscholar +1 more source
Single-molecule fingerprinting of protein-drug interaction using a funneled biological nanopore
Nature Communications, 2023 In drug discovery, efficient screening of protein-drug interactions (PDIs) is hampered by the limitations of current biophysical approaches. Here, we develop a biological nanopore sensor for single-molecule detection of proteins and PDIs using the pore ...
Ki-Baek Jeong +11 more
semanticscholar +1 more source
De-novo Assembly of Limnospira fusiformis Using Ultra-Long Reads
Frontiers in Microbiology, 2021 The Limnospira genus is a recently established clade that is economically important due to its worldwide use in biotechnology and agriculture. This genus includes organisms that were reclassified from Arthrospira, which are commercially marketed as ...
McKenna Hicks +3 more
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