Results 41 to 50 of about 30,309 (268)

Nanopore SimulatION – a raw data simulator for Nanopore Sequencing [PDF]

2018 IEEE International Conference on Bioinformatics and Biomedicine (BIBM), 2018
Nanopore DNA sequencing enables the sequence determination of single DNA molecules up to 10,000 times longer than currently permitted by second-generation sequencing platforms. Nanopore sequencing gives real-time access to sequencing data and enables the detection of epigenetic modifications.
Christian Rohrandt, Nadine Kraft, Pay Gießelmann, Bjorn Brandl, Bernhard M. Schuldt, Ulrich Jetzek, Franz-Josef Müller   +6 more
openaire   +3 more sources

De novo design of nanopores for single-molecule detection that incorporates α-helix peptides [PDF]

Nanopore sensing using transmembrane proteins enjoys great potential for rapid and low-cost single-molecule detection. Creating pore-forming proteins or peptides is an emerging frontier in the field of nanopore sensing.
Masataka, Usami, Batsaikhan, Mijiddorj, Izuru, Kawamura, Zugui, Peng, Yusuke, Sekiya, Ryuji, Kawano   +5 more
core   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Strain-induced fabrication of anisotropic nanopores by in-situ E-beam irradiation

Journal of Materials Research and Technology
Anisotropic nanopores are essential to regulate materials' anisotropic performance including thermal conductivity, ion blocking and so on. Nevertheless, fabricating anisotropic nanopores remains challenging because the formation mechanism is ambiguous ...
Weiwei Xia, Yu Wang, Quan An, Lianyang Chen, Ran Cai, Liang Chu   +5 more
doaj   +1 more source

Phase Behavior in Nanopores and Its Indication for Cyclic Gas Injection in a Volatile Oil Reservoir from Duvernay Shale [PDF]

, 2022
Duvernay shale spans over 6 million acres with a total resource of 440 billion barrels’ oil equivalent in the Western Canada Sedimentary Basin (WCSB). The oil recovery factors typically decrease with the decreasing of gas-oil ratio (GOR) in oil window of
Li Liu, Wei Yu, Xiangwen Kong, Ping Wang, Wenjing Zhang, Hongjun Wang   +5 more
core   +1 more source

Identification and characterisation of calcitonin receptor isoforms expressed in glioblastoma derived glioma stem and U‐87 MG cells

FEBS Open Bio, EarlyView.
Glioblastoma cells express calcitonin receptor variants (CT receptor isoforms) that may help them survive stress. Using qPCR, transcript‐specific long‐read nanopore sequencing, immunofluorescence co‐localisation and comparative sequence analysis, this study identifies a novel alternatively spliced CALCR transcript that encodes the CTb receptor isoform ...
Pragya Gupta, Sebastian GB Furness, Tahereh Gharbi, Ric De Paoli‐Iseppi, Shweta S. Joshi, Michael Clark, David L. Hare, Peter Wookey   +7 more
wiley   +1 more source

Enhanced thermostability of enzymes accommodated in thermo-responsive nanopores

, 2012
The crowded and hydrophobic microenvironment was created for immobilized enzymes via the thermally-initiated shrinkage of PNIPAM polymers anchored in the nanopores of mesoporous silica.
Jin, Qianru, 杨启华, Liu, Jia, Li, Can, Yang, Qihua, 李灿, Bai, Shiyang   +6 more
core   +1 more source

3D Flow Field Measurements Outside Nanopores [PDF]

, 2021
We demonstrate a non-stereoscopic, video-based particle tracking system with optical tweezers to study fluid flow in 3D in the vicinity of glass nanopores.
Andresen, Kurt, Kurt Andresen, Alice L. Thorneywork, Thorneywork, Alice L., Mc Hugh, Jeffrey, Hugh, Jeffrey Mc, Jeffrey Mc Hugh, Ulrich F. Keyser, Keyser, Ulrich F.   +8 more
core   +1 more source

Compound Heterozygote Friedreich Ataxia Patients With Covert Proximal FXN Gene Deletions

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT We present Friedreich ataxia patients with frataxin gene deletions. Data and records were collected at the Children's Hospital of Philadelphia from patients enrolled in the FACOMS natural history study. Patients with proximal deletions initially diagnosed with only one GAA expanded allele had more severe disease than their homozygous expansion
Michael P. Lazaropoulos, Morgan C. Devore, Christina Lam, Courtney Park, Sanjay Bidichandani, David R. Lynch   +5 more
wiley   +1 more source

Scan-Rate-Dependent Ion Current Rectification in Bipolar Interfacial Nanopores

Micromachines
This study presents a theoretical investigation into the voltammetric behavior of bipolar interfacial nanopores due to the effect of potential scan rate (1–1000 V/s). Finite element method (FEM) is utilized to explore the current–voltage (I–V) properties
Xiaoling Zhang, Yunjiao Wang, Jiahui Zheng, Chen Yang, Deqiang Wang   +4 more
doaj   +1 more source