Results 101 to 110 of about 1,205,213 (312)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Natural Selection, Irrationality and Monopolistic Competition [PDF]

This paper builds an evolutionary model of an industry where firms produce differentiated products. Firms have different average cost functions and different demand functions.
Luo, Guo Ying
core   +1 more source

Why do we age? The main signs of aging

Aktualʹnì Pitannâ Farmacevtičnoï ì Medičnoï Nauki ta Praktiki
Aging is a gradual and irreversible pathophysiological process. This is manifested in the decline of tissue and cell functions and a significant variety of pathologies associated with aging, including neurodegenerative diseases, cardiovascular diseases ...
Ugіs Kletnieks, Audrius Butkevichius, V. V. Hladyshev, S. S. Sоkolovskyi   +3 more
doaj   +1 more source

Detecting Selection on Protein Stability through Statistical Mechanical Models of Folding and Evolution

Biomolecules, 2014
The properties of biomolecules depend both on physics and on the evolutionary process that formed them. These two points of view produce a powerful synergism.
Ugo Bastolla
doaj   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Concepts of Drift and Selection in “The Great Snail Debate” of the 1950s and Early 1960s [PDF]

, 2007
Recently, much philosophical discussion has centered on the best way to characterize the concepts of random drift and natural selection, and, in particular, on the question of whether selection and drift can be conceptually distinguished (Beatty 1984 ...
Millstein, Roberta L.
core  

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Sexy streamers? The role of natural and sexual selection in the evolution of hirundine tail streamers [PDF]

, 2001
In this comment we review some experiments, which address the initial selection pressures promoting the development of tail streamers in some hirundine species.
Buchanan, Katherine Louise, Evans, Matthew, Park, Kirsty   +2 more
core   +1 more source

Population genomic evidence of structured and connected Plasmodium vivax populations under host selection in Latin America

Ecology and Evolution
Pathogen genomic epidemiology has the potential to provide a deep understanding of population dynamics, facilitating strategic planning of interventions, monitoring their impact, and enabling timely responses, and thereby supporting control and ...
Johanna Helena Kattenberg, Pieter Monsieurs, Julie De Meyer, Katlijn De Meulenaere, Erin Sauve, Thaís C. deOliveira, Marcelo U. Ferreira, Dionicia Gamboa, Anna Rosanas‐Urgell   +8 more
doaj   +1 more source