Native Plasmid-Encoded Mercury Resistance Genes Are Functional and Demonstrate Natural Transformation in Environmental Bacterial Isolates [PDF]
mSystems, 2019 Plasmid-mediated horizontal gene transfer (HGT) is a major driver of genetic diversity in bacteria. We experimentally validated the function of a putative mercury resistance operon present on an abundant 8-kbp native plasmid found in groundwater samples ...
Ankita Kothari +5 more
doaj +7 more sources
Biochemical Properties and Roles of DprA Protein in Bacterial Natural Transformation, Virulence, and Pilin Variation. [PDF]
J Bacteriol, 2023 Natural transformation enables bacteria to acquire DNA from the environment and contributes to genetic diversity, DNA repair, and nutritional requirements. DNA processing protein A (DprA) receives incoming single-stranded DNA and assists RecA loading for
Sharma DK +3 more
europepmc +2 more sources
Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms. [PDF]
Nat Commun, 2022 SCCmec is a large mobile genetic element that includes the mecA gene and confers resistance to β-lactam antibiotics in methicillin-resistant Staphylococcus aureus (MRSA).
Maree M +5 more
europepmc +2 more sources
ComFC mediates transport and handling of single-stranded DNA during natural transformation. [PDF]
Nat Commun, 2022 The ComFC protein is essential for natural transformation, a process that plays a major role in the spread of antibiotic resistance genes and virulence factors across bacteria. However, its role remains largely unknown.
Damke PP +12 more
europepmc +2 more sources
Natural transformation and cell division delay in competent Staphylococcus aureus [PDF]
Microbiology Spectrum, 2023 Genetic competence for natural transformation, considered one of the three main mechanisms leading to horizontal gene transfer in bacteria, is able to promote evolution, through genomic plasticity, and foster antibiotic resistance and virulence factors ...
Fedy Morgene +4 more
doaj +2 more sources
Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri. [PDF]
Pathogens, 2021 Aliarcobacter butzleri is an emergent enteropathogen, showing high genetic diversity, which likely contributes to its adaptive capacity to different environments.
Bonifácio M +7 more
europepmc +2 more sources
Interbacterial Transfer of Carbapenem Resistance and Large Antibiotic Resistance Islands by Natural Transformation in Pathogenic Acinetobacter. [PDF]
mBio, 2022 Acinetobacter baumannii is a multidrug-resistant pathogen responsible for difficult-to-treat hospital-acquired infections. Understanding the mechanisms leading to the emergence of the multidrug resistance in this pathogen today is crucial.
Godeux AS +6 more
europepmc +2 more sources
Basic Characterization of Natural Transformation in Avibacterium paragallinarum [PDF]
Microbiology Spectrum, 2023 Avibacterium paragallinarum is the pathogen involved in infectious coryza (IC), an acute infectious upper respiratory disease in chickens. The prevalence of IC has increased in China in recent years.
Donghui Liu +8 more
doaj +2 more sources
Generation of miniploid cells and improved natural transformation procedure for a model cyanobacterium Synechococcus elongatus PCC 7942 [PDF]
Frontiers in Microbiology, 2022 The biotechnologically important and naturally transformable cyanobacterium, Synechococcus elongatus PCC 7942, possesses multiple genome copies irrespective of its growth rate or condition.
Sadaf Riaz +7 more
doaj +2 more sources
NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens. [PDF]
Commun Biol, 2022 The fast-growing bacterium Vibrio natriegens has recently gained increasing attention as a novel chassis organism for fundamental research and biotechnology.
Stukenberg D, Hoff J, Faber A, Becker A.
europepmc +2 more sources