Biochemical Properties and Roles of DprA Protein in Bacterial Natural Transformation, Virulence, and Pilin Variation. [PDF]
J Bacteriol, 2023 Natural transformation enables bacteria to acquire DNA from the environment and contributes to genetic diversity, DNA repair, and nutritional requirements. DNA processing protein A (DprA) receives incoming single-stranded DNA and assists RecA loading for
Sharma DK +3 more
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Natural transformation allows transfer of SCCmec-mediated methicillin resistance in Staphylococcus aureus biofilms. [PDF]
Nat Commun, 2022 SCCmec is a large mobile genetic element that includes the mecA gene and confers resistance to β-lactam antibiotics in methicillin-resistant Staphylococcus aureus (MRSA).
Maree M +5 more
europepmc +2 more sources
Automating Cloning by Natural Transformation [PDF]
ACS Synthetic Biology, 2020 Affordable and automated cloning platforms are essential to many synthetic biology studies. However, the traditional E. coli-based cloning is a major bottleneck as it requires heat shock or electroporation implemented in the robotic workflows. To overcome this problem, we explored bacterial natural transformation for automatic DNA cloning and ...
Tilmann Weber +9 more
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Natural transformation and cell division delay in competent Staphylococcus aureus [PDF]
Microbiology Spectrum, 2023 Genetic competence for natural transformation, considered one of the three main mechanisms leading to horizontal gene transfer in bacteria, is able to promote evolution, through genomic plasticity, and foster antibiotic resistance and virulence factors ...
Fedy Morgene +4 more
doaj +2 more sources
Natural Transformation as a Mechanism of Horizontal Gene Transfer in Aliarcobacter butzleri. [PDF]
Pathogens, 2021 Aliarcobacter butzleri is an emergent enteropathogen, showing high genetic diversity, which likely contributes to its adaptive capacity to different environments.
Bonifácio M +7 more
europepmc +2 more sources
Interbacterial Transfer of Carbapenem Resistance and Large Antibiotic Resistance Islands by Natural Transformation in Pathogenic Acinetobacter. [PDF]
mBio, 2022 Acinetobacter baumannii is a multidrug-resistant pathogen responsible for difficult-to-treat hospital-acquired infections. Understanding the mechanisms leading to the emergence of the multidrug resistance in this pathogen today is crucial.
Godeux AS +6 more
europepmc +2 more sources
NT-CRISPR, combining natural transformation and CRISPR-Cas9 counterselection for markerless and scarless genome editing in Vibrio natriegens. [PDF]
Commun Biol, 2022 The fast-growing bacterium Vibrio natriegens has recently gained increasing attention as a novel chassis organism for fundamental research and biotechnology.
Stukenberg D, Hoff J, Faber A, Becker A.
europepmc +2 more sources
Generation of miniploid cells and improved natural transformation procedure for a model cyanobacterium Synechococcus elongatus PCC 7942 [PDF]
Frontiers in Microbiology, 2022 The biotechnologically important and naturally transformable cyanobacterium, Synechococcus elongatus PCC 7942, possesses multiple genome copies irrespective of its growth rate or condition.
Sadaf Riaz +7 more
doaj +2 more sources
Effect of Nutritional Determinants and TonB on the Natural Transformation of Riemerella anatipestifer [PDF]
Frontiers in Microbiology, 2021 Riemerella anatipestifer is a gram-negative bacterium that is the first naturally competent bacterium identified in the family Flavobacteriaceae. However, the determinants that influence the natural transformation and the underlying mechanism remain ...
Li Zhang +55 more
doaj +2 more sources
The DNA transporter ComEC has metal-dependent nuclease activity that is important for natural transformation. [PDF]
Mol Microbiol, 2021 In the process of natural transformation bacteria import extracellular DNA molecules for integration into their genome. One strand of the incoming DNA molecule is degraded, whereas the remaining strand is transported across the cytoplasmic membrane.
Silale A, Lea SM, Berks BC.
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