Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Assessing the effectiveness of direct gesture interaction for a safety critical maritime application [PDF]
Multi-touch interaction, in particular multi-touch gesture interaction, is widely believed to give a more natural interaction style. We investigated the utility of multi-touch interaction in the safety critical domain of maritime dynamic positioning (DP)
Eva Hornecker +5 more
core +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Evaluating interaction design and user experience in augmented reality: a systematic review
BackgroundAugmented Reality (AR) technologies are rapidly advancing, offering new opportunities for interactive and immersive user experiences. However, the success of AR applications depends significantly on thoughtful interaction design and robust ...
Claire L. Hughes, Waldemar Karwowski
doaj +1 more source
Early‐life exposure to a high‐fat diet altered intact Achilles tendons in rat offspring, making them thinner, stiffer, and molecularly distinct even without injury. These findings suggest that developmental high‐fat diet exposure may impair tendon quality and increase susceptibility to mechanical overload or tendon injury later in life.
Heyong Yin +3 more
wiley +1 more source
Natural User Interface Design using Multiple Displays for Courier Dispatch Operations. [PDF]
This thesis explores how Natural User Interface (NUI) interaction and Multiple Display Technology (MDT) can be applied to an existing Freight Management System (FMS), to improve the command and control interface of the dispatch operators.
Clifford, Rory
core +1 more source
Versatile vector tools for efficient protein screening across multiple expression systems
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu +5 more
wiley +1 more source
Natural Language User Interface For Software Engineering Tasks [PDF]
In this paper, we present the idea to use natural language as the user interface for programming tasks. Programming languages assist with repetitive tasks that involve the use of conditionals, loops and statements. This is what is often challenging users.
Wachtel, Alexander +2 more
core +2 more sources
Aquaporin‐3 and aquaporin‐5 impact the development of pancreatic ductal adenocarcinoma spheroids
Schematic representation of the role of aquaporin‐3 (AQP3) and aquaporin‐5 (AQP5) in pancreatic ductal adenocarcinoma (PDAC). Both proteins are upregulated in PDAC and are associated with tumor progression and metastatic potential. Silencing AQP3 or AQP5 in PDAC spheroids results in decreased diameter, area, and overall growth, underscoring their key ...
Catarina Pimpão +3 more
wiley +1 more source
Flexible physical interfaces [PDF]
Human-computer interface devices are rigid, and afford little or no opportunity for end-user adaptation. This thesis proposes that valuable new interaction possibilities can be generated through the development of user interface hardware that is ...
Villar, Nicolas, Nicolas Villar
core

