Results 51 to 60 of about 221,642 (264)

Matrix metalloproteinase‐9 regulates cell adhesion and membrane protrusive activity of ovarian cancer cells

FEBS Open Bio, EarlyView.
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel, Sam Bennett, Natalie Ehlinger, Nelson Cook, Katelyn Marvin, Caleb L. Lines, Sam Reyes, Brooke Kirby, Shawn M. Ellerbroek   +8 more
wiley   +1 more source

Suppression of lung adenocarcinoma migration through organelle alkalization by human lactoferrin – albumin fusion

FEBS Open Bio, EarlyView.
This paper reveals how human lactoferrin–albumin fusion (hLF‐HSA) potently suppresses lung adenocarcinoma cell migration. hLF‐HSA upregulates NHE7, leading to Golgi alkalization, disruption of the Golgi secretome, downregulation of MMP1, and reversal of EMT. These findings suggest a novel Golgi‐targeting strategy to suppress cancer cell migration.
Hana Nopia, Masahiro Kimura, Daisuke Kurimoto, Atsushi Sato   +3 more
wiley   +1 more source

Digital Twins for 3D Confocal Microscopy: Near-Field, Far-Field, and Comparison with Experiments

Sensors
To push the boundaries of confocal microscopy beyond its current limitations by predicting sensor responses for complex surface geometries, we build digital twins using three rigorous models, the finite element method (FEM), Fourier modal method (FMM ...
Poul-Erik Hansen, Tobias Pahl, Liwei Fu, Ida Nielsen, Felix Rosenthal, Stephan Reichelt, Peter Lehmann, Astrid Tranum Rømer   +7 more
doaj   +1 more source

Identification of Near-Field and Far-Field Using Confocal Microscopy Setup for Optical Sensing Applications

ASM Science Journal, 2019
The presence of near-field indicates the existence of evanescent waves, which is one of the important requirements for the development of an optical sensor.
Wan Maisarah Mukhtar, Nor Zahirah Mohd Zulkifli, Razman Mohd Halim   +2 more
doaj   +1 more source

Long‐term hippocampal alterations and cognitive impairment in a murine model of surgical sepsis

FEBS Open Bio, EarlyView.
Using a mouse model of surgical sepsis, we tested long‐term memory and analyzed the transcriptome of single cells isolated from the hippocampus. Survivor mice showed worse memory, loss of certain brain cell subpopulations, and abnormal immune cell activity—suggesting that post‐sepsis brain alterations may be linked to cognitive deficits.
Dong Seong Cho, Rebecca Schmitt, Aneesha Dasgupta, Alexandra Ducharme, Jason Doles   +4 more
wiley   +1 more source

Scattering-type scanning near-field optical microscopy with low-repetition-rate pulsed light source through phase-domain sampling

Nature Communications, 2016
Low repetition rate lasers are suitable for studying nonlinear optical phenomena, while near-field microscopy allows high spatial resolution for nanomaterial characterisation. Here, Wang et al.
Haomin Wang, Le Wang, Xiaoji G. Xu
doaj   +1 more source

Near-field optical microscopy: Towards the molecular scale

, 2023
Near-field scanning optical microscopy (NSOM) is one of the most recent scanning probe techniques. In this technique, an optical probe is brought in the vicinity of the sample surface, in the near-field zone. The microscope can either work in illumination mode, in which the probe consists of a sub-wavelength light source, or in collection mode, in ...
openaire   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Scanning Probe Microscopy as a Tool for Investigation of Biomaterials

Advances in Electrical and Electronic Engineering, 2012
Super-microscopic techniques like scanning tunnelling microscopy, atomic force microscopy or scanning near-field optical microscopy allows investigate micro- and/or nano-scale surfaces and structures.
Veronika Novotna, Alexandr Knapek, Pavel Tomanek, Sarka Safarova   +3 more
doaj   +1 more source

RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells

FEBS Open Bio, EarlyView.
RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour, Christine Volmert, Valentina Piano   +2 more
wiley   +1 more source