Results 51 to 60 of about 188,865 (243)

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Hybrid‐Frequency Power Loop to Eliminate Negative‐Sequence Current for Star‐Connected Cascaded H‐Bridge STATCOM

IET Power Electronics
Since the power grid frequently experiences asymmetric operation caused by the unbalanced power load, the current compensation for the negative‐sequence component is typically required to control the star‐connected cascaded H‐bridge STATCOM. However, the
Zhendong Ji, Kangli Liu, Wei Liu, Yichao Sun, Dongye Li, Jianhua Wang, Xiaodan Wu, Jianfeng Zhao   +7 more
doaj   +1 more source

Fault location in the observation matrix of collection lines for grid-connected wind power supported by grid-forming energy storage

International Journal of Electrical Power & Energy Systems
Aiming at the problem that the accuracy of fault location in the collection line supported by energy storage for wind power grid connection is easily affected by the number of measurement points and the access of power electronic equipment, a fault ...
Hongchun Shu, Bowen Wang, Jun Dong, Yutao Tang   +3 more
doaj   +1 more source

Compatibility Issues with Irregular Current Injection Islanding Detection Methods and a Solution

Energies, 2019
Islanding detection methods, based on injecting high-/low-frequency currents or negative sequence fundamental frequency currents and observing the resultant responses, are collectively referred to as irregular current injection methods in this paper.
Menghua Liu, Wei Zhao, Qing Wang, Songling Huang, Kunpeng Shi   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Discussion of Auxiliary Power Source Open-Phase Protection in Nuclear Power Plant

南方能源建设
[Objective] The auxiliary power source in nuclear power plant operates at no-load normally, the protection cannot alarm when it occurs single phase open, but this open-phase condition will threaten the nuclear safety seriously, it should assemble special
Shunhua WU, Houjun QIAN, Tianwei WANG, Yupeng SUN, Jiangang CHEN, Chuanyang CAO, Jianfeng DONG, Zhenhua ZHAO   +7 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Avidin is evolutionarily conserved in fish but dispensable for development and resistance against Streptococcus agalactiae in zebrafish

FEBS Open Bio, EarlyView.
The presence of biotin‐binding avidin proteins in fish and their biological significance are poorly characterized. We cataloged fish avidins and demonstrate that they are widely present and evolutionarily conserved. We created avd knockout zebrafish and show that zebavidin is dispensable for development and that resistance of avd knockout embryos in ...
Anni K. Saralahti, Markus J. T. Ojanen, Mataleena Parikka, Otto Kauko, Mika Rämet, Vesa P. Hytönen   +5 more
wiley   +1 more source

Fluorination: A strategy to tune the interactions of molecules and nanoscale systems with biological matter

FEBS Open Bio, EarlyView.
This minireview explores fluorination as a key strategy to optimize therapeutic molecules and nanocarriers. By incorporating different fluorinated motifs—from single atoms to linear and branched chains—drug delivery, cellular uptake, and colloidal stability can be improved and fine‐tuned.
Martina Beccalli, Beatrice Lucia Bona, Francesca Baldelli Bombelli   +2 more
wiley   +1 more source

Ofatumumab in Myelin Oligodendrocyte Glycoprotein Antibody–Associated Disease: A Comparison With Rituximab

Annals of Clinical and Translational Neurology, EarlyView.
ABSTRACT Objective To evaluate the efficacy and safety of ofatumumab in patients with myelin oligodendrocyte glycoprotein antibody–associated disease (MOGAD), and compare it with rituximab. Methods We conducted a single–center, observational study including 22 MOGAD patients treated with ofatumumab and 21 treated with rituximab.
Yuxin Fan, Lei Zhou, Jingzi ZhangBao, Hongmei Tan, Zhouzhou Wang, Chao Quan   +5 more
wiley   +1 more source