Results 151 to 160 of about 1,269 (164)

Nesprin-1 impact on tumorigenic cell phenotypes

Molecular Biology Reports, 2019
The largest protein of the nuclear envelope (NE) is Nesprin-1 which forms a network along the NE interacting with actin, Emerin, Lamin, and SUN proteins. Mutations in the SYNE1 gene and reduction in Nesprin-1 protein levels have been reported to correlate with several age related diseases and cancer.
Ilknur Sur-Erdem, Muhammed Sajid Hussain, Maria Asif, Nareg Pınarbası, Ali Cenk Aksu, Angelika A. Noegel   +5 more
openaire   +2 more sources

Nesprin-2 epsilon: A novel nesprin isoform expressed in human ovary and Ntera-2 cells

Biochemical and Biophysical Research Communications, 2011
The nuclear envelope-associated cytoskeletal protein, nesprin-2, is encoded by a large gene containing several internal promoters that produce shorter isoforms. In a study of Ntera-2 teratocarcinoma cells, a novel isoform, nesprin-2-epsilon, was found to be the major mRNA and protein product of the nesprin-2 gene.
Lam, Le Thanh, Boehm, Sabrina V., Roberts, Roland G., Morris, Glenn E.   +3 more
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Nesprins in Cell Stability and Migration

2014
Nesprins are a family of proteins that are primarily known for their localization along the nuclear envelope. Together with inner nuclear membrane SUN proteins, they form the core of the LINC (Linker of Nucleoskeleton and Cytoskeleton) complex that traverses both nuclear membranes to connect the cytoplasm and the nuclear interior.
Sascha, Neumann, Angelika A, Noegel
openaire   +2 more sources

Detection of Diverse and High Molecular Weight Nesprin-1 and Nesprin-2 Isoforms Using Western Blotting

2016
Heavily utilized in cell and molecular biology, western blotting is considered a crucial technique for the detection and quantification of proteins within complex mixtures. In particular, the detection of members of the nesprin (nuclear envelope spectrin repeat protein) family has proven difficult to analyze due to their substantial isoform diversity ...
James, Carthew, Iakowos, Karakesisoglou
openaire   +2 more sources

Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis

2018
The biochemical characterization of proteins most often require their identification by immunoblotting. Whereas SDS-PAGE provides satisfactory results for most proteins, the identification of larger proteins requires alternative methods to ensure their separation and complete transfer onto nitrocellulose membranes.
Chloe, Potter, Didier, Hodzic
openaire   +2 more sources

Nuclear alignment in myotubes requires centrosome proteins recruited by nesprin-1

Journal of Cell Science, 2016
ABSTRACT Myotubes are syncytial cells generated by fusion of myoblasts. Among the numerous nuclei in myotubes of skeletal muscle fibres, the majority are equidistantly positioned at the periphery, except for clusters of multiple nuclei underneath the motor endplate.
Aude Espigat-Georger, Vyacheslav Dyachuk, Cécile Chemin, Laurent Emorine, Andreas Merdes   +4 more
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[NESPRINS--nuclear envelope proteins ensuring integrity].

Tsitologiia, 2015
This review describes the nesprins (nuclear envelope spectrin-repeat proteins), which are recently discovered family of nuclear envelope proteins. These proteins play an important role in maintaining the cellular architecture and establish the link between the nucleus and other sub-cellular compartments.
E G, Pershina, K N, Morozova, E V, Kiseleva   +2 more
openaire   +1 more source

Serum level of c-Abl, nestin, nesprin-1, and nesprin-2 in asthmatic patients

Journal of Allergy and Clinical Immunology, 2023
Nazia Habib, Thomas Brown, Sarah McMullan, Muhammad Pasha, Dale Tang   +4 more
openaire   +1 more source

Nesprin-3: a versatile connector between the nucleus and the cytoskeleton

Biochemical Society Transactions, 2011
The cytoskeleton is connected to the nuclear interior by LINC (linker of nucleoskeleton and cytoskeleton) complexes located in the nuclear envelope. These complexes consist of SUN proteins and nesprins present in the inner and outer nuclear membrane respectively.
Mirjam, Ketema, Arnoud, Sonnenberg
openaire   +2 more sources

Nesprin isoforms: are they inside or outside the nucleus?

Biochemical Society Transactions, 2010
The giant isoforms of nesprins 1 and 2 are emerging as important players in cellular organization, particularly in the positioning of nuclei, and possibly other organelles, within the cytoplasm. The experimental evidence suggests that nesprins also occur at the inner nuclear membrane, where they interact with the nuclear lamina.
Glenn E, Morris, K Natalie, Randles
openaire   +2 more sources