Results 261 to 270 of about 142,834 (335)

Rapid generation of prion disease models using AAV‐delivered PrP variants in knockout mice

Brain Pathology, EarlyView.
We developed a rapid AAV‐based system to generate prion disease models in weeks rather than months. Following systemic AAV9P31 delivery of modified PrP to knockout mice, we achieved brain‐wide expression and successful propagation of both classical (RML) and atypical (GSS‐A117V) prion strains.
Maitena San‐Juan‐Ansoleaga, Eva Fernández‐Muñoz, Jorge M. Charco, Enric Vidal, Diego Herrero‐Martínez, Josu Galarza‐Ahumada, Cristina Sampedro‐Torres‐Quevedo, Samanta Giler, Mariví Geijo, Gloria González‐Aseguinolaza, Hasier Eraña, Joaquín Castilla   +11 more
wiley   +1 more source

Correction to: UPrimer: A Clade-Specific Primer Design Program Based on Nested-PCR Strategy and Its Applications in Amplicon Capture Phylogenomics. [PDF]

Mol Biol Evol
europepmc   +1 more source

Exploring mosquito abundance and Plasmodium infection through nested-PCR: implications for disease surveillance and control. [PDF]

Sci Rep
Abbas H, Sajid MS, Rizwan HM, Tahir UB, Farooqi SH, Iqbal Z, Malik MA, Yaseen K, Maqbool M, Raza FA, Raza M, Fouad D, Ataya FS.   +12 more
europepmc   +1 more source

3,4‐Methylenedioxymethamphetamine (MDMA) does not induce robust psychomotor activation and 50‐kHz ultrasonic vocalisations in tryptophan hydroxylase 2 (Tph2)‐deficient rats lacking serotonin in the central nervous system

British Journal of Pharmacology, EarlyView.
Background and Purpose 3,4‐Methylenedioxymethamphetamine (MDMA), commonly known as ecstasy, is a psychostimulant with entactogenic properties and known to induce arousal and euphoria. As an amphetamine derivate, MDMA acts on the monoamine systems in the brain and stimulates release of dopamine (DA), noradrenaline (NA) and serotonin (5‐HT), yet their ...
Tianhua Wang, Rainer K. W. Schwarting, Markus Wöhr   +2 more
wiley   +1 more source

Raising the bar: genus-specific nested PCR improves detection and lineage identification of avian haemosporidian parasites. [PDF]

Front Cell Infect Microbiol
Musa S, Hemberle T, Bensch S, Palinauskas V, Baltrūnaitė L, Woog F, Mackenstedt U.   +6 more
europepmc   +1 more source

Effects of Antibiotic Treatment on the Results of Nested PCRs for Scrub Typhus

, 2008
Dong‐Min Kim, Joo Nam Byun
openalex   +1 more source

Bruton tyrosine kinase (Btk) in neutrophils is indispensable for initiating and maintaining skin inflammation in a model of pemphigoid diseases

British Journal of Pharmacology, EarlyView.
Background and Purpose Bruton tyrosine kinase (Btk) is essential for B cell function. Its role in myeloid cells is less understood. Greater insights into Btk significance in myeloid cells are needed to evaluate its potential as a therapeutic target during the effector phase of antibody‐induced autoimmune diseases, where inhibiting autoantibody ...
Henning Olbrich, Paul Schilf, Sripriya Murthy, Sina Gonther, Mareike Neumann, Christoph M. Hammers, Jasper N. Pruessmann, Christian D. Sadik   +7 more
wiley   +1 more source

Establishment of Nested PCR for the Detection of Pseudomonas plecoglossicida and Epidemiological Survey of Larimichthys crocea in the Southeast Coastal Region. [PDF]

Animals (Basel)
Duan X, Li J, Shi H, Tao Z, Wei X, Ye Y, Guo B.   +6 more
europepmc   +1 more source

Identification and Phylogenetic Characterisation of Novel Adeno‐Associated Virus Capsids in Non‐Human Primate Tissues

Cell Proliferation, EarlyView.
AAV's widespread application is currently confronting critical challenges. This study established a systematic framework of ‘natural discovery–evolutionary analysis–functional optimization’, providing a new paradigm for the development of next‐generation AAV vectors with clinical‐grade tissue specificity, low immunogenicity, and cross‐species ...
Liyu Zhu, Kai Xu, Yali Ding, Kailun Liu, Jing Liu, Zongren Hou, Rui Niu, Ning Yang, Hualing Qin, Baoyang Hu, Ying Zhang, Wei Li   +11 more
wiley   +1 more source

Environmental DNA for invasive rat surveillance and biosecurity on islands

Conservation Science and Practice, EarlyView.
We collected aquatic eDNA samples from beach and lake sites on three atoll islands with different invasion statuses before and after a rat eradication operation. Two dozen eDNA samples can confirm with ≥95% probability the presence of invasive rats prior to any management intervention. However, following the eradication operation, neither targeted eDNA
Sebastian Steibl, Shaun P. Wilkinson, David J. Will, Neil J. Gemmell, James C. Russell   +4 more
wiley   +1 more source