Results 271 to 280 of about 1,037,260 (308)

Detection of Theileria orientalis in Iran by semi-nested PCR

Parasitology Research, 2011
In order to identify and differentiate Theileria orientalis in cattle which may be infected with Theileria annulata simultaneously, a semi-nested PCR was performed. Thus, 160 blood samples were collected from apparently healthy native cattle in Golestan province of northern Iran, during 2009 to 2011. The Tbs-S/Tbs-A primer set derived from the 18S rRNA
Peyman, Ghaemi, Nasser, Hoghooghi-Rad, Parviz, Shayan, Brigitte, Eckert   +3 more
openaire   +2 more sources

Diagnosis of Oropouche virus infection by RT‐nested‐PCR

Journal of Medical Virology, 2001
AbstractUsing the RT‐PCR with primers that anneal to the 5′ and the 3′ extremities of the genome segments of bunyaviruses and internal primers that anneal to the S segment of Simbu serogroup viruses in a nested PCR it was possible to amplify the Oropouche virus (ORO) genome from the sera of three patients.
Marcos Lázaro, Moreli, Victor Hugo, Aquino, Ana Cecília R, Cruz, Luiz Tadeu M, Figueiredo   +3 more
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Rapid HLA‐DRB1 genotyping by nested PCR amplification

Tissue Antigens, 1992
Abstract: State of the art genotyping of HLA class II alleles with group‐specific DNA amplification by the polymerase chain reaction (PCR) (1) and subsequent probing with sequence‐specific oligonucleotides (2–4) is not suitable for typing cadaveric organ donors since the typing procedure takes far more than one working day.
G, Bein, R, Gläser, H, Kirchner
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A rapid hemi-nested PCR for HTLV-I detection

Journal of Clinical Virology, 2003
A hemi-nested PCR approach was adopted to detect HTLV-1 infection in clinical samples of peripheral blood mononuclear cells (PBMCs) from subjects with positive or indeterminate serological results. Our results showed that the hemi-nested PCR quickly solved the diagnostic query, detecting the presence of proviral HTLV-1 DNA in two of the 252 patients ...
Davide Gibellini, Paola Monari, Monica Sassi, Maria Carla Re   +3 more
openaire   +2 more sources

Nested PCR and Multiplex Nested PCR

2004
David Tarragó‐Asensio, Ana Avellón‐Calvo   +1 more
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Nested PCR

2007
Opisana je metoda nested PCR koja se sastoji od dvije reakcije PCR u kojima se koriste dva različita para početnica.
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Nested PCR

2011
BONIN, Serena, DOTTI, ISABELLA
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Nested PCR ve Kullanım Alanları

2013
Nested PCR, PCR tekniğinin hassasiyetini artırmak için geliştirilen en popular yöntemlerden biridir. İstenilen DNA dizisinin bulunup çoğaltılmasında hassasiyeti 104 kat artırdığı tahmin edilmektedir. Bu reaksiyon mekanizmasında birbirini takip eden iki polimeraz zincir reaksiyonunu içermektedir. İlk amplifikasyonda hedef DNA’nın dış bölgesine özgün iki
SEVİNDİK, Emre, ABACI, Z. Tuğba
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Lariat-Dependent Nested PCR for Flanking Sequence Determination

2010
Methods detailed in this chapter relate to the use of Lariat-dependent Nested (LaNe) PCR to characterize unknown RNA or DNA sequence flanking known regions. A multitude of approaches designed to determine flanking sequences have been described in the literature.
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Evaluation of nested PCR assay for the detection Aspergillus species

2009
In this research, we investigated the application of nested PCR assay for the identification of Aspergillus species. We obtained amplified products from fungal genera other than Aspergillus, showing the presence of cross-reactions with fungal DNA.
ABACI, Özlem, HALİKİ-UZTAN, Alev, DEMİR, Özgür   +2 more
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