Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Diagnostic Challenge of Small Bowel Neuroendocrine Tumour Mimicking Biliary Disease: A Case Report. [PDF]
Nazir B +4 more
europepmc +1 more source
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley +1 more source
Temporary carbon dioxide removal to offset short-lived climate forcers. [PDF]
He Y +5 more
europepmc +1 more source
Planning for shortages? Net Present Value analysis for a deteriorating item with partial backlogging
Yousef Ghiami, P. Beullens
semanticscholar +1 more source
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel +4 more
wiley +1 more source
Exploring potential value of neutrophil extracellular traps in major depressive disorder. [PDF]
Shi Y +17 more
europepmc +1 more source
Consumer Preferences for Annuity Attributes: Beyond Net Present Value
Suzanne B. Shu +2 more
semanticscholar +1 more source
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Evaluating the social return on investment of nature-based social prescribing to alleviate loneliness: a randomized controlled trial in Barcelona. [PDF]
Papon V +12 more
europepmc +1 more source

