Results 21 to 30 of about 195,460 (295)

HIV-1 Envelope and MPER Antibody Structures in Lipid Assemblies

Cell Reports, 2020
Summary: Structural and functional studies of HIV envelope glycoprotein (Env) as a transmembrane protein have long been complicated by challenges associated with inherent flexibility of the molecule and the membrane-embedded hydrophobic regions. Here, we
Kimmo Rantalainen, Zachary T. Berndsen, Aleksandar Antanasijevic, Torben Schiffner, Xi Zhang, Wen-Hsin Lee, Jonathan L. Torres, Lei Zhang, Adriana Irimia, Jeffrey Copps, Kenneth H. Zhou, Young D. Kwon, William H. Law, Chaim A. Schramm, Raffaello Verardi, Shelly J. Krebs, Peter D. Kwong, Nicole A. Doria-Rose, Ian A. Wilson, Michael B. Zwick, John R. Yates, III, William R. Schief, Andrew B. Ward   +22 more
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Neutralizing antiviral antibody responses

, 2001
Neutralizing antibodies are evolutionarily important effectors of immunity against viruses. Their evaluation has revealed a number of basic insights into specificity, rules of reactivity (tolerance), and memory—namely, (1) Specificity of neutralizing antibodies is defined by their capacity to distinguish between virus serotypes; (2) B cell reactivity ...
Zinkernagel, R, LaMarre, A, Ciurea, A, Hunziker, L, Ochsenbein, A, McCoy, K, Fehr, T, Bachmann, M, Kalinke, U, Hengartner, H   +9 more
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Neutralizing antibodies against coronaviruses [PDF]

Microscopy and Microanalysis, 2021
Neutralizing monoclonal antibodies against SARS-CoV-2 are being used as therapeutics against COVID-19. The Bjorkman laboratory is investigating the mechanisms of virus neutralization by human antibodies that bind the SARSCoV- 2 spike protein. Using structural techniques, including single-particle cryoelectron microscopy and X-ray crystallography, the ...
openaire   +2 more sources

Coupling of HIV-1 gp120-derived Core Protein to Paramagnetic Beads and Adsorption Assays

Bio-Protocol, 2015
Analysis of the functional activity in polyclonal serum following immunization of a complex protein or glycoprotein immunogen is a very important but tedious process.
Jidnyasa Ingale, Richard Wyatt
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Mapping Polyclonal Antibody Responses in Non-human Primates Vaccinated with HIV Env Trimer Subunit Vaccines

Cell Reports, 2020
Summary: Rational immunogen design aims to focus antibody responses to vulnerable sites on primary antigens. Given the size of these antigens, there is, however, potential for eliciting unwanted, off-target responses. Here, we use our electron microscopy
Bartek Nogal, Matteo Bianchi, Christopher A. Cottrell, Robert N. Kirchdoerfer, Leigh M. Sewall, Hannah L. Turner, Fangzhu Zhao, Devin Sok, Dennis R. Burton, Lars Hangartner, Andrew B. Ward   +10 more
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Pre-existing immunity with high neutralizing activity to 2009 pandemic H1N1 influenza virus in Shanghai population. [PDF]

PLoS ONE, 2013
Pre-existing immunity is an important factor countering the pandemic potential of an emerging influenza virus strain. Thus, studying of pre-existing immunity to the 2009 pandemic H1N1 virus (2009 H1N1) will advance our understanding of the pathogenesis ...
Xiaoqing Liu, Yuan Liu, Yanjun Zhang, Zhihui Chen, Ziwei Tang, Qingqiang Xu, Yue Wang, Ping Zhao, Zhongtian Qi   +8 more
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Use of Transient Transfection for cGMP Manufacturing of eOD-GT8 60mer, a Self-Assembling Nanoparticle Germline-Targeting HIV-1 Vaccine Candidate

Pharmaceutics
We describe the current Good Manufacturing Practice (cGMP) production and subsequent characterization of eOD-GT8 60mer, a glycosylated self-assembling nanoparticle HIV-1 vaccine candidate and germline targeting priming immunogen.
Vaneet K. Sharma, Sergey Menis, Evan T. Brower, Eddy Sayeed, Jim Ackland, Angela Lombardo, Christopher A. Cottrell, Jonathan L. Torres, Thomas Hassell, Andrew B. Ward, Vadim Tsvetnitsky, William R. Schief   +11 more
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Neutralizing antibody fails to impact the course of Ebola virus infection in monkeys. [PDF]

PLoS Pathogens, 2007
Prophylaxis with high doses of neutralizing antibody typically offers protection against challenge with viruses producing acute infections. In this study, we have investigated the ability of the neutralizing human monoclonal antibody, KZ52, to protect ...
Wendelien B Oswald, Thomas W Geisbert, Kelly J Davis, Joan B Geisbert, Nancy J Sullivan, Peter B Jahrling, Paul W H I Parren, Dennis R Burton   +7 more
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Structure and immunogenicity of a stabilized HIV-1 envelope trimer based on a group-M consensus sequence

Nature Communications, 2019
Stabilized, native-like trimers of the HIV envelope protein, such as SOSIP trimers, are potential antigens for an HIV vaccine. Here, the authors generate a SOSIP trimer based on the consensus sequence of group M isolates, determine its structure and ...
Kwinten Sliepen, Byung Woo Han, Ilja Bontjer, Petra Mooij, Fernando Garces, Anna-Janina Behrens, Kimmo Rantalainen, Sonu Kumar, Anita Sarkar, Philip J. M. Brouwer, Yuanzi Hua, Monica Tolazzi, Edith Schermer, Jonathan L. Torres, Gabriel Ozorowski, Patricia van der Woude, Alba Torrents de la Peña, Mariëlle J. van Breemen, Juan Miguel Camacho-Sánchez, Judith A. Burger, Max Medina-Ramírez, Nuria González, Jose Alcami, Celia LaBranche, Gabriella Scarlatti, Marit J. van Gils, Max Crispin, David C. Montefiori, Andrew B. Ward, Gerrit Koopman, John P. Moore, Robin J. Shattock, Willy M. Bogers, Ian A. Wilson, Rogier W. Sanders   +34 more
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An antibody against Siglec-15 promotes bone formation and fracture healing by increasing TRAP+ mononuclear cells and PDGF-BB secretion

Bone Research, 2021
Osteoporosis (OP) is a common age-related disease characterized by a deterioration of bone mass and structure that predisposes patients to fragility fractures.
Gehua Zhen, Yang Dan, Ruomei Wang, Ce Dou, Qiaoyue Guo, Melissa Zarr, Linda N. Liu, Lieping Chen, Ruoxian Deng, Yusheng Li, Zengwu Shao, Xu Cao   +11 more
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