This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill +4 more
wiley +1 more source
New Species of Babinskaiidae (Insecta: Neuroptera) From the Mid-Cretaceous of Myanmar and the Morphological Divergence of the Family Across the Cretaceous. [PDF]
Lu X, Luo Y, Zhuo, Liu X.
europepmc +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
New localities of Berberis croatica Horvat in Croatia
The Croatian barberry (Berberis croatica Horvat) is an endemic Illyrian-Balkanic species growing on rocky slopes at the upper limit of the wooded zone in Croatia, Bosnia and Herzegovina,Montenegro, and Macedonia.
Ivan Kosalec +7 more
core
New records and ecological insights of <i>Spondylus limbatus</i> (Bivalvia, Spondylidae) from the Panamic Province. [PDF]
Cotom-Nimatuj JA +12 more
europepmc +1 more source
Matrix metalloproteinase‐9 (MMP9) drives ovarian cancer progression. Using MMP9‐null cells (M9‐KO) created from ovarian cancer cells, we found MMP9 loss did not block Epidermal Growth Factor (EGF)‐driven E‐cadherin dissolution or EMT but delayed and reduced EGF‐driven membrane protrusions. Transient MMP9 re‐expression drove membrane protrusion.
Claire Strauel +8 more
wiley +1 more source
Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke +4 more
wiley +1 more source
Genetic and Environmentally Induced Scalation Variation in Bisexual and Parthenogenetic Lizards. [PDF]
Tarkhnishvili D +3 more
europepmc +1 more source
Systemic dysregulation of apolipoproteins in amyotrophic lateral sclerosis serum
Amyotrophic lateral sclerosis (ALS) is a fatal disease that damages motor neurons. This study found that people with ALS show significant changes in blood fats and the proteins that carry them. Several apolipoproteins were higher, lipid balances were altered, and normal protein–lipid relationships were disrupted.
Finula I. Isik +6 more
wiley +1 more source
Diversity and distribution of gastrointestinal nematodes in three sympatric rodent taxa from South Africa. [PDF]
Schlemmer E +3 more
europepmc +1 more source

