Results 211 to 220 of about 973,339 (276)

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

FEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

A genomic approach for accurate identification of closely related species with next-generation sequencing samples. [PDF]

Sci Rep
Dain Marzouka NA, Al-Aamri A, Alshamsi F, Khalili M, Chehadeh SEH, Mohamed MS, Eltahir YM, Koliyan R, Abdelhalim MM, Attia A, Mousa M, Tay G, Alsafar H.   +12 more
europepmc   +1 more source

Microglial dynamics and ferroptosis induction in human iPSC‐derived neuron–astrocyte–microglia tri‐cultures

FEBS Open Bio, EarlyView.
A tri‐culture of iPSC‐derived neurons, astrocytes, and microglia treated with ferroptosis inducers as an Induced ferroptosis model was characterized by scRNA‐seq, cell survival, and cytokine release assays. This analysis revealed diverse microglial transcriptomic changes, indicating that the system captures key aspects of the complex cellular ...
Hongmei Lisa Li, Hiroko Ohmiya, Sou Sakamoto, Masato Yugami, Akiko Oki, Makoto Furusawa, Yan Ling   +6 more
wiley   +1 more source

Antimicrobial use on <i>Campylobacter</i> revealed by next-generation sequencing in patients with common variable immunodeficiency. [PDF]

Front Microbiol
Jehanne Q, Conrad A, Josserand RN, Benech N, Schatz PM, Bénéjat L, Ducournau A, Aptel J, Martin M, Jauvain M, Dauwalder O, Lehours P.   +11 more
europepmc   +1 more source

Multi‐omics and low‐input proteomics profiling reveals dynamic regulation driving pluripotency initiation in early mouse embryos

FEBS Open Bio, EarlyView.
Mouse pre‐implantation development involves a transition from totipotency to pluripotency. Integrating transcriptomics, epigenetic profiling, low‐input proteomics and functional assays, we show that eight‐cell embryos retain residual totipotency features, whereas cytoskeletal remodeling regulated by the ubiquitin‐proteasome system drives progression ...
Wanqiong Li, Xi Xiao, Lingbin Qi, Chanyi Li, Sirui Song, Jiaying Qin, Zhigang Xue, Jinfeng Xue, Huaifang Li   +8 more
wiley   +1 more source

Comparison of tissue acquisition techniques for Next-Generation Sequencing of non-small cell lung cancer (NSCLC). [PDF]

BMJ Open Respir Res
Bhamani A, Naidu SB, Adroja N, Rogers L, Anandan L, Bennett P, Ahmad T, Forster MD, Ahmed A, Thakrar R, Janes SM, Moore DA, Navani N.   +12 more
europepmc   +1 more source

HIV‐1 establishes immediate latency in T cells expressing the viral Nef protein

FEBS Open Bio, EarlyView.
Nef is a viral protein often omitted from HIV‐1 reporter viruses. Consequently, its role in viral latency is unclear. We developed three novel dual reporter HIV‐1 derivatives that express Nef and allow for detection of latent and productive infection. Using these reporters, we show that Nef does not affect the establishment of immediate viral latency ...
Cindy Lam, Ivan Sadowski
wiley   +1 more source

Editorial: Application of next-generation sequencing in clinical settings. [PDF]

Front Genet
Chai Z, Mei H, Li Q, Maqsood I, Cai J, Li MM, Xu F, Zhao X.   +7 more
europepmc   +1 more source

BMI‐1 modulation and trafficking during M phase in diffuse intrinsic pontine glioma

FEBS Open Bio, EarlyView.
The schematic illustrates BMI‐1 phosphorylation during M phase, which triggers its translocation from the nucleus to the cytoplasm. In cycling cells, BMI‐1 functions within the PRC1 complex to mediate H2A K119 monoubiquitination. Following PTC596‐induced M phase arrest, phosphorylated BMI‐1 dissociates from PRC1 and is exported to the cytoplasm via its
Banlanjo Umaru, Deepak Kumar Mishra, Shiva Senthil Kumar, Hao‐Han Pang, Brendan Devine, Komal Khan, Rachid Drissi   +6 more
wiley   +1 more source

MGDB: A Novel Bioinformatics Quality Control Tool for Clinical Next-Generation Sequencing. [PDF]

Cancer Inform
Gayap HT, Robichaud PP, Crapoulet N, Allain EP.   +3 more
europepmc   +1 more source