Results 111 to 120 of about 110,342 (137)
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The chloroplast genome of Nicotiana sylvestris and Nicotiana tomentosiformis: complete sequencing confirms that the Nicotiana sylvestris progenitor is the maternal genome donor of Nicotiana tabacum

Molecular Genetics and Genomics, 2006
The tobacco cultivar Nicotiana tabacum is a natural amphidiploid that is thought to be derived from ancestors of Nicotiana sylvestris and Nicotiana tomentosiformis. To compare these chloroplast genomes, DNA was prepared from isolated chloroplasts from green leaves of N. sylvestris and N. tomentosiformis, and subjected to whole-genome shotgun sequencing.
Masahiro Sugiura   +2 more
openaire   +3 more sources

Production of Nicotiana tabacum x Nicotiana acuminata hybrid by ovule culture [PDF]

open access: possiblePlant Cell Reports, 1986
Using a conventional sexual crossing technique, Nicotiana tabacum x N. acuminata was not produced. After the fertilized ovules were cultured for 20 days in a liquid Nitsch H medium, germination was observed. The roots grew rapidly but leaves did not.
Kazuo Nakata   +3 more
openaire   +2 more sources

In vitro hybridization in an incompatible cross Nicotiana glutinosa X Nicotiana megalosiphon [PDF]

open access: possibleTheoretical and Applied Genetics, 1985
Using conventional techniques interspecific hybridization between N. glutinosa (2n=24) and N. megalosiphon (2n=40) results in failure while the reciprocal is successful. Attempts were made here to obtain an F1 hybrid of N. glutinosa X N. megalosiphon by embryo rescue.
U. Subhashini   +3 more
openaire   +2 more sources

<i>Nicotiana</i> (<i>Nicotiana tobaccum, Nicotiana benthamiana</i>)

2006
Agrobacterium-mediated transformation of Nicotiana species, namely, Nicotiana tobaccum and Nicotiana benthamiana, using leaf disks as the target explant has provided the plant community with a valuable tool for rapid evaluation of transgenes in higher plants.
openaire   +3 more sources

Amplification of repetitive DNA from Nicotiana plumbaginifolia in asymmetric somatic hybrids between Nicotiana sylvestris and Nicotiana plumbaginifolia

Theoretical and Applied Genetics, 1993
Asymmetric somatic hybrids were obtained between a chlorophyll-deficient mutant of Nicotiana sylvestris (V42) and a nitrate-reductase (NR)-deficient line of N. plumbaginifolia (cnx20 or Nia26), using each of the parents alternately as the irradiated donor. Irradiation doses applied ranged from 10 to 1,000 Gy of gamma-rays. Hybrid selection was based on
Yuri Gleba   +3 more
openaire   +3 more sources

Analysis of RNA Silencing in Agroinfiltrated Leaves of Nicotiana Benthamiana and Nicotiana Tabacum

Plant Molecular Biology, 2005
In this study we analyse several aspects of cytoplasmic RNA silencing by agroinfiltration of DNA constructs encoding single- and double-stranded RNAs derived from a GFP transgene and from the endogenous Virp1 gene. Both types of inductors resulted after 2-4 days in much higher concentration of siRNAs in the agroinfiltrated zone than normally seen ...
Edyta Kościańska   +4 more
openaire   +3 more sources

Peroxidases in the genus Nicotiana

Theoretical and Applied Genetics, 1970
Leaf peroxidases of 60 Nicotiana species, 19 cultivars, autopolyploids, interspecific hybrids, and amphidiploids have been compared by polyacrylamide gel block electrophoresis. At least 19 peroxidase bands, four cathodic and 15 anodic, were detected in the species which varied from two bands in N. arentsii to 12 bands in N. tabacum.
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Sieve plate pores of nicotiana

Journal of Ultrastructure Research, 1969
Nicotiana tabacum L. (var. Wisconsin 38) plants were fixed for light and electron microscopy using solutions of glutaraldehyde, acrolein, and formaldehydeglutaraldehyde. The plants were (a) cut before fixation at room temperature or (b) fixed as whole plants at room temperature, or (c) rapidly frozen and transferred to cold fixative. Good fixation was
Richard Anderson, James Cronshaw
openaire   +4 more sources

THE SPECIES NICOTIANA RUSTICA – CULTURED REPRESENTATIVE OF THE GENUS NICOTIANA

Естественные и технические науки, 2020
Вид Nicotiana rustica L., махорка представитель семейства Solanaceae, рода Nicotiana, однолетнее растение, имеющее несколько разновидностей. Встречаются махорки зеленые и желтые в зависимости от окраски листьев, крупнолистные и мелколистные, высокорослые, средне- и мелкорослые. Выделяется шесть основных форм пластинки листа сердцевидная, треугольная,
openaire   +1 more source

Genetic instability in somatic hybrids of Nicotiana tabacum and Nicotiana knightiana

Molecular and General Genetics MGG, 1978
Somatic hybrids of Nicotiana knightiana (2n=2X=24) and an albino mutant of Nicotiana tabacum (2n=4X=48) were selected after polyethylene glycol induced protoplast fusion. Three lines were selected on the basis of the simultaneous expression of shoot inducibility and green pigmentation, traits originally separated in the parental species.
Pál Maliga   +3 more
openaire   +2 more sources

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