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Curtis's Botanical Magazine, 2018
SummaryNicotiana benthamiana is described and illustrated from plants collected in northwestern Western Australia and cultivated at the Royal Botanic Gardens, Kew. It is widely used as a laboratory plant in plant virus studies, and we also discuss its cytology, cultivation and natural ecology.
Mark W. Chase +1 more
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SummaryNicotiana benthamiana is described and illustrated from plants collected in northwestern Western Australia and cultivated at the Royal Botanic Gardens, Kew. It is widely used as a laboratory plant in plant virus studies, and we also discuss its cytology, cultivation and natural ecology.
Mark W. Chase +1 more
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2019
Published as part of Andersen, Trine Bundgaard, Rasmussen, Silas Anselm, Christensen, Søren Brøgger & Simonsen, Henrik Toft, 2019, Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy, pp. 168-174 in Phytochemistry 157 on page 169, DOI: 10.1016/j.phytochem.2018.10.027, http://zenodo.org/record ...
Andersen, Trine Bundgaard +3 more
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Published as part of Andersen, Trine Bundgaard, Rasmussen, Silas Anselm, Christensen, Søren Brøgger & Simonsen, Henrik Toft, 2019, Biosynthesis of tovarol and other sesquiterpenoids in Thapsia laciniata Rouy, pp. 168-174 in Phytochemistry 157 on page 169, DOI: 10.1016/j.phytochem.2018.10.027, http://zenodo.org/record ...
Andersen, Trine Bundgaard +3 more
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Nicotiana (Nicotiana tobaccum, Nicotiana benthamiana).
Methods in molecular biology (Clifton, N.J.), 2006Agrobacterium-mediated transformation of Nicotiana species, namely, Nicotiana tobaccum and Nicotiana benthamiana, using leaf disks as the target explant has provided the plant community with a valuable tool for rapid evaluation of transgenes in higher plants.
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Reconstitution of Metabolic Pathway in Nicotiana benthamiana
2021Reconstitution of metabolite biosynthesis pathway plays a pivotal role in functional characterization of biosynthesis enzymes and metabolite bioengineering. Traditionally, metabolic pathways are reconstituted in bacteria or yeast due to their ease for genetic manipulation and transformation.
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Transient Transactivation Studies in Nicotiana benthamiana Leaves
2018Transitory gene expression systems in Nicotiana benthamiana leaves, in combination with the use of gene silencing suppressors as the p19 or HC-pro proteins that allow for elevated levels of gene expression, have proven to be a highly versatile tool to analyze transcriptional function of DNA binding factors in the activated or repressed expression of ...
Pilar, Lasierra, Salomé, Prat
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Stable Plastid Transformation in Nicotiana benthamiana
Journal of Plant Biology, 2009Plastids from Nicotiana benthamiana were transformed with the vector for dicistronic expression of two genes—aminoglycoside 3'-adenyltransferase (aadA) and green fluorescent protein (gfp)—in the plastids of Nicotiana tabacum. Transplastomic shoots exhibited green fluorescence under UV light.
Seyed Javad Davarpanah +6 more
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Targeted Insertion in Nicotiana benthamiana Genomes via Protoplast Regeneration
2023Insertion of a specific sequence in a targeted region for precise editing is still a major challenge in plants. Current protocols rely on inefficient homology-directed repair or non-homologous end-joining with modified double-stranded oligodeoxyribonucleotides (dsODNs) as donors.
Fu-Hui, Wu +2 more
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Engineering montbretin A biosynthesis in Nicotiana benthamiana
2021The plant kingdom has always been a useful source of therapeutic molecules for humans. Montbretin A (MbA) is a complex acylated flavonol glycoside found in the underground storage organs, called corms, of the ornamental plant montbretia (Crocosmia x crocosmiiflora). MbA was discovered as a highly efficient and specific inhibitor of the human pancreatic
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Characterization of in vivo functions of Nicotiana benthamiana RabE1
Planta, 2012We characterized the gene expression, subcellular localization, and in vivo functions of a Nicotiana benthamiana small GTPase belonging to the RabE family, designated NbRabE1. The NbRabE1 promoter drove strong β-glucuronidase reporter expression in young tissues containing actively dividing cells and in stomata guard cells.
Chang Sook, Ahn +2 more
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