Results 281 to 290 of about 25,977 (305)
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2021
Aims. The use of plants as a recombinant peptides expression system is a valuable alternative for the production of collagen for various medical and scientific purposes. The aim of this work was to obtained transgenic plants of Nicotiana benthamiana with two human genes encoding subunits of prolyl-4-hydroxylase (P4H), responsible for key ...
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Aims. The use of plants as a recombinant peptides expression system is a valuable alternative for the production of collagen for various medical and scientific purposes. The aim of this work was to obtained transgenic plants of Nicotiana benthamiana with two human genes encoding subunits of prolyl-4-hydroxylase (P4H), responsible for key ...
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2021
Set of vectors has been created for plastome transformation of plants of Nicotiana genera. These constructs possess esxA and fbpB genes combined with selective aadA gene which are expressed polycistronicly under control of psbA gene. Over 30 antibiotic resistant lines have been selected in the course of N. benthamiana plastome transformation and a part
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Set of vectors has been created for plastome transformation of plants of Nicotiana genera. These constructs possess esxA and fbpB genes combined with selective aadA gene which are expressed polycistronicly under control of psbA gene. Over 30 antibiotic resistant lines have been selected in the course of N. benthamiana plastome transformation and a part
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High-level expression of Camelid nanobodies in Nicotiana benthamiana
Transgenic Research, 2009Nanobodies (or VHHs) are single-domain antigen-binding fragments derived from Camelid heavy chain-only antibodies. Their small size, monomeric behaviour, high stability and solubility, and ability to bind epitopes not accessible to conventional antibodies make them especially suitable for many therapeutic and biotechnological applications.
Yi-Hui Audrey, Teh, Tony A, Kavanagh
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Analysis of RNA Silencing in Agroinfiltrated Leaves of Nicotiana Benthamiana and Nicotiana Tabacum
Plant Molecular Biology, 2005In this study we analyse several aspects of cytoplasmic RNA silencing by agroinfiltration of DNA constructs encoding single- and double-stranded RNAs derived from a GFP transgene and from the endogenous Virp1 gene. Both types of inductors resulted after 2-4 days in much higher concentration of siRNAs in the agroinfiltrated zone than normally seen ...
Edyta, Kościańska +4 more
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Studying Secretory Protein Synthesis in Nicotiana benthamiana Protoplasts
Transient gene expression in plant protoplasts facilitates the analysis of hybrid genes in a fast and reproducible manner. The technique is particularly powerful when studying basic conserved biochemical processes including de novo protein synthesis, modification, assembly, transport, and turnover.Jing, An, Jurgen, Denecke
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Cocksfoot mottle virus P1 suppresses RNA silencing in Nicotiana benthamiana and Nicotiana tabacum
Virus Research, 2007The Sobemovirus genome consists of positive sense, single-stranded polycistronic RNA. The 5'-terminal ORF, encoding the protein P1, is its most variable region. Sobemoviral P1 has been described as dispensable for replication but indispensable for systemic infection. The P1 of Rice yellow mottle virus-Nigerian isolate (RYMV-N) is the only RNA silencing
Cecilia, Sarmiento +4 more
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2021
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