Results 21 to 30 of about 46,351 (298)

The role of the Cucumber mosaic virus 2b protein in viral movement and symptom induction [PDF]

open access: yes, 2009
The Cucumber mosaic virus (CMV) 2b protein is a counter-defense factor and symptom determinant. Conserved domains in the 2b protein sequence were mutated in the 2b gene of strain Fny-CMV.
Chen H.-Y.   +15 more
core   +2 more sources

Protein Immunoprecipitation Using Nicotiana benthamiana Transient Expression System

open access: yesBio-Protocol, 2015
Nicotiana benthamiana (N. benthamiana) is a useful model system to transiently express protein at high level. This protocol describes in detail how to transiently express protein in N.
Fang Xu, Charles Copeland, Xin Li
doaj   +1 more source

A Thioredoxin Domain-Containing Protein Interacts with Pepino mosaic virus Triple Gene Block Protein 1 [PDF]

open access: yes, 2018
Pepino mosaic virus (PepMV) is a mechanically-transmitted tomato pathogen of importance worldwide. Interactions between the PepMV coat protein and triple gene block protein (TGBp1) with the host heat shock cognate protein 70 and catalase 1 (CAT1 ...
Khechmar, Souheyla   +8 more
core   +2 more sources

Characterization of VRC01, a potent and broadly neutralizing anti-HIV mAb, produced in transiently and stably transformed tobacco [PDF]

open access: yes, 2014
The proposed clinical trial in Africa of VRC01, a potent broadly neutralizing antibody (bNAb) capable of neutralizing 91% of known HIV-1 isolates, raises concerns about testing a treatment which will be too expensive to be accessible by the most ...
EMEA   +4 more
core   +1 more source

Reactions of Nicotiana species to inoculation with monopartite and bipartite begomoviruses

open access: yesVirology Journal, 2011
Background Some Nicotiana species are widely used as experimental hosts for plant viruses. Nicotiana species differ in ploidy levels, chromosome numbers and have diverse geographical origins.
Mansoor Shahid   +2 more
doaj   +1 more source

Transient Expression of Tetrameric Recombinant Human Butyrylcholinesterase in Nicotiana benthamiana. [PDF]

open access: yes, 2016
To optimize the expression, extraction and purification of plant-derived tetrameric recombinant human butyrylcholinesterase (prBChE), we describe the development and use of plant viral amplicon-based gene expression system; Tobacco Mosaic Virus (TMV) RNA-
Alkanaimsh, Salem   +13 more
core   +1 more source

An RxLR effector from phytophthora infestans prevents re-localisation of two plant NAC transcription factors from the endoplasmic reticulum to the nucleus [PDF]

open access: yes, 2013
The plant immune system is activated following the perception of exposed, essential and invariant microbial molecules that are recognised as non-self. A major component of plant immunity is the transcriptional induction of genes involved in a wide array ...
A Block   +70 more
core   +4 more sources

RNA-binding protein immunoprecipitation as a tool to investigate plant miRNA processing interference by regulatory proteins of diverse origin [PDF]

open access: yes, 2018
Background: Due to the nature of viral RNA genomes, RNA viruses depend on many RNA-binding proteins (RBP) of viral and host origin for replication, dissemination and evasion of host RNA degradation pathways.
Garcia, Maria Laura   +2 more
core   +2 more sources

Genome and transcriptome characterization of the glycoengineered Nicotiana benthamiana line ΔXT/FT

open access: yesBMC Genomics, 2019
Background The allotetraploid tobacco species Nicotiana benthamiana native to Australia has become a popular host for recombinant protein production. Although its usage grows every year, little is known on this plant’s genomic and transcriptomic features.
Matteo Schiavinato   +4 more
doaj   +1 more source

Efficient in planta production of amidated antimicrobial peptides that are active against drug-resistant ESKAPE pathogens

open access: yesNature Communications, 2023
Antimicrobial peptides (AMPs) are next-generation antibiotics that can be used to combat drugresistant pathogens. Here, the authors report efficient production of bioactive amidated AMPs by transient expression in Nicotiana benthamiana line expressing ...
Shahid Chaudhary   +13 more
doaj   +1 more source

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