Results 41 to 50 of about 27,473 (251)

In Vivo Biosynthesis and Direct Incorporation of Non‐Canonical Amino Acids into Proteins

ChemBioChem, Accepted Article.
Autonomous cells are engineered biological systems capable of biosynthesising and directly incorporating non‐canonical amino acids (ncAAs) into proteins. These systems have the potential to extend the applicability of the genetic code to enable large‐scale fermentative production of proteins carrying ncAAs.
Ivana Drienovska, Jan Hendrik Illies, T. Moritz Weber   +2 more
wiley   +1 more source

Shifting the Substrate Scope of an Ene/Yne‐Reductase by Loop Engineering

ChemCatChem, EarlyView.
A flexible active‐site loop that is involved in shaping the substrate and product cleft as well as the active‐site pocket was identified for the MDR‐related ene/yne‐reductase CaeEnR1 from Cyclocybe aegerita. Double mutations of this loop showed an up to 20‐fold increase in conversion rates toward cinnamaldehyde‐like substrates and a 2‐fold increase in ...
Dominik Karrer, Erika Wedler, Carolin Mewe, Martin Gand, Marian Vogt, Lukas Korf, Lars‐Oliver Essen, Martin Rühl   +7 more
wiley   +1 more source

In Vitro Live Cell Imaging Reveals Nuclear Dynamics and Role of the Cytoskeleton During Asymmetric Division of Pollen Mitosis I in Nicotiana Benthamiana

Cytoskeleton, EarlyView.
ABSTRACT Pollen is a male gametophyte of angiosperms. Following meiosis, the microspore undergoes an asymmetric division called pollen mitosis I (PMI), which produces two cells of different sizes: a large vegetative cell and a small generative cell. Polarized nuclear migration and positioning during PMI are important for successful pollen development ...
Yoko Mizuta, Masako Igarashi, Tomomi Shinagawa, Ikuma Kaneshiro, Haoto Anan, Daisuke Kurihara   +5 more
wiley   +1 more source

Redução dos sintomas causados pela Xylella fastidiosa subsp. pauca por meio de aplicação de benzotiadiazole e silício Redution of the symptons caused by Xylella fastidiosa subsp. pauca through application of benzothiadiazole and silicon

Pesquisa Agropecuária Brasileira, 2007
O objetivo deste trabalho foi avaliar o efeito da aplicação do benzotiadiazole (BTH) e do silício sobre o controle da doença causada pela Xylella fastidiosa subsp. pauca em Nicotiana tabacum.
Juliana Camargo Martinati, Paulo Teixeira Lacava, Sandra Kazue Shishido Miyasawa, Sylvia Dias Guzzo, João Lúcio Azevedo, Siu Mui Tsai   +5 more
doaj   +1 more source

Nicotiana tabacum

, 1956
Published as part of Becherer, 1956, Florae Vallesiacae Supplementum, pp.
openaire   +2 more sources

Structure of a Polyubiquitin Gene in Nicotiana tabacum

Molecules and Cells, 1998
Using a tobacco ubiquitin cDNA clone as a probe, a genomic clone in EMBL3 coding for a tobacco polyubiquitin protein was isolated. Southern blot hybridization of the genomic clone with the cDNA clone identified a BamHI/EcoRI fragment of 2.5 kb to contain the coding region of polyubiquitin, and thus the fragment was subcloned into a plasmid vector ...
S M, Park, J H, Kim, C B, Hong
openaire   +2 more sources

Coproporphyrinogenase in tobacco (Nicotiana tabacum L.) [PDF]

Biochemical Journal, 1970
1. Coproporphyrinogenase was extracted and purified from tobacco (Nicotiana tabacum L.). Enzyme activity was mainly located in mitochondria rather than in chloroplasts. The enzyme was purified by differential centrifugation, ammonium sulphate fractionation, calcium phosphate gel adsorption and dialysis. A 69-fold final purification was obtained. 2.
GW Miller, W. P. Hsu
openaire   +3 more sources

METTL5‐mediated 18S rRNA m6A modification enhances ribosome assembly and ABA response in Arabidopsis

iMeta, EarlyView.
METTL5 catalyzes the N6‐methyladenosine (m6A) methylation at A1771 in 18S rRNA, a modification essential for its association with the ribosomal protein RPL24A, facilitating the assembly of 80S ribosome. This facilitates the translation of mRNAs encoding the detoxifying glutathione S‐transferase (GST) enzymes, thereby maintaining normal reactive oxygen ...
Ping Li, Yu Zhang, Songyao Zhang, Jinqi Ma, Sheng Fan, Lisha Shen   +5 more
wiley   +1 more source

Computational Study on the Reaction Mechanism of 5‐Enolpyruvylshikimate‐3‐phosphate Synthase from Nicotiana Tabacum

ChemistryOpen, EarlyView.
The reaction mechanism of 5‐enolpyruvylshikimate‐3‐phosphate synthase from Nicotiana tabacum (NtEPSPS) is elucidated using quantum chemical calculations. The proposed mechanism involves four sequential steps: carbocation intermediate formation, tetrahedral intermediate formation, rate‐limiting C−O bond cleavage, and C=C bond re‐formation.
Qingfang Han, Beibei Lin, Ziwei Liu, Mengsha Li, Zhaopeng Luo, Xixian Xie, Lijuan Ma, Hao Su, Xiang Sheng   +8 more
wiley   +1 more source

Interactions of rare earth elements with living organisms and emerging biotechnical applications

PLANTS, PEOPLE, PLANET, EarlyView.
Rare earth elements (REEs) are critical resources required to achieve net‐zero carbon emission targets and energy security. However, rising demand for REEs coupled with significant extraction and processing challenges and geopolitical risks restricts access to REE resources.
Samantha A. McGaughey, Shagufta Iqbal, Annamaria De Rosa, Jessica A. Caley, Anna H. Kaksonen, Denys Villa‐Gomez, Caitlin S. Byrt   +6 more
wiley   +1 more source