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Frameshifts close to the Klebsiella pneumoniae nifH promoter prevent multicopy inhibition by hybrid nifH plasmids

Molecular and General Genetics MGG, 1987
Certain multicopy plasmids bearing promoter sequences of nitrogen fixation (nif) genes inhibit expression of chromosomal genes in Nif+ Klebsiella pneumoniae, hence leading to a Nif- phenotype. This 'multicopy inhibition' has been attributed to the titration of the nif-specific activator protein NifA by the plasmid-borne promoter sequences.
M, Buck, W, Cannon
openaire   +2 more sources

nifH homologs from soil metagenome

Ekologija, 2011
Biological nitrogen fixation is an important process that allows nitrogen uptake into biological systems. But it is still unclear from where the first nif gene was formed or evolved. Soil samples from different agro-climatic zones of western Indian Himalaya were analysed for the diversity of nifH in their metagenome.
Ravindra Soni, Reeta Goel
openaire   +1 more source

EndophyticnifHgene diversity in African sweet potato

Canadian Journal of Microbiology, 2003
A cultivation-independent approach was used to identify potentially nitrogen-fixing endophytes in seven sweet potato varieties collected in Uganda and Kenya. Nitrogenase reductase genes (nifH) were amplified by PCR, and amplicons were cloned in Escherichia coli.
Birgit, Reiter   +3 more
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Nucleotide sequence of the gene encoding the nitrogenase iron protein (nifH) of Azospirillum brasilense and identification of a region controlling nifH transcription

Molecular and General Genetics MGG, 1989
The DNA sequence was determined for the Azospirillum brasilense nifH gene and part of the nifD gene. The nifH gene is 885 bp long and encodes 293 amino acid residues. The region upstream of the nifH open reading frame contains a putative promoter whose sequence shows perfect homology with promoters of other diazotrophic bacteria and two putative ...
BAZZICALUPO, MARCO   +5 more
openaire   +3 more sources

Distribution of Extensive nifH Gene Diversity Across Physical Soil Microenvironments

Microbial Ecology, 2006
The diversity of nitrogen-fixing bacteria is well described for aquatic environments; however, terrestrial analyses remain mostly biased to rhizobial plant-microbe associations. We maximized the level of resolution for this study through the use of nucleotide sequence information extracted from a series of soil microenvironments, ranging from ...
Izquierdo, JA, Nusslein, K
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Fluorescence in situ hybridization for intracellular localization of nifH mRNA

Systematic and Applied Microbiology, 2009
Few reports on in situ mRNA detection in bacteria have been published, even though a major aim in environmental microbiology is to link function/activity to the identity of the organisms. This study reports a reliable approach for the in situ detection of nifH mRNA using fluorescence hybridization based on a previously described protocol for pmoA. nifH
Martin, Pilhofer   +4 more
openaire   +2 more sources

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