Results 1 to 10 of about 4,632 (165)

nifH gene expression and diversity in geothermal springs of Tengchong, China [PDF]

Frontiers in Microbiology, 2022
Terrestrial hot springs have been suggested to harbor diverse diazotrophic lineages by using DNA-based nifH gene phylogenetic analysis. However, only a small amount of diazotrophs were ever confirmed to perform nitrogen fixation.
Zhao-Qi Song, Li Wang, Feng Liang, Qingfeng Zhou, Dongli Pei, Hongchen Jiang, Wen-Jun Li   +6 more
doaj   +6 more sources

A comprehensive evaluation of PCR primers to amplify the nifH gene of nitrogenase. [PDF]

PLoS ONE, 2012
The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed
John Christian Gaby, Daniel H Buckley
doaj   +6 more sources

Seasonal and long-term effects of nutrient additions and liming on the nifH gene in cerrado soils under native vegetation [PDF]

iScience, 2021
Summary: Biological nitrogen fixation (BNF) represents the main input source of N in tropical savannas. BNF could be particularly important for Brazilian savannas (known as Cerrado) that show a highly conservative N cycle.
Rafaella Silveira, Thiago de Roure Bandeira de Mello, Maria Regina Silveira Sartori, Gabriel Sérgio Costa Alves, Fernando Campos de Assis Fonseca, Carla Simone Vizzotto, Ricardo Henrique Krüger, Mercedes Maria da Cunha Bustamante   +7 more
doaj   +2 more sources

Evaluation of Primers Targeting the Diazotroph Functional Gene and Development of NifMAP – A Bioinformatics Pipeline for Analyzing nifH Amplicon Data

Frontiers in Microbiology, 2018
Diazotrophic microorganisms introduce biologically available nitrogen (N) to the global N cycle through the activity of the nitrogenase enzyme. The genetically conserved dinitrogenase reductase (nifH) gene is phylogenetically distributed across four ...
Roey Angel, Maximilian Nepel, Christopher Panhölzl, Hannes Schmidt, Craig W. Herbold, Stephanie A. Eichorst, Dagmar Woebken   +6 more
doaj   +3 more sources

Sediments as Potential Sources of Non-Cyanobacterial Diazotrophs in Arctic Sea Ice and Seawater. [PDF]

Environ Microbiol Rep
Diazotrophic communities across Arctic environments: Integrated analyses of diazotrophs in sea ice, seawater, and sediments show that Arctic continental shelf sediments function as a central reservoir, supplying diazotrophs to both sea ice and seawater through resuspension processes.
Bo H, Zhang Z, Fujiwara A, Fukai Y, Ito M, Kimura S, Oggier M, Waga H, Whitmore L, Hamasaki K, Shiozaki T.   +10 more
europepmc   +2 more sources

Genetic analysis of the NifM dependence of the nitrogenase iron proteins [PDF]

mBio
NifH maturation in certain diazotrophic bacteria depends on NifM, a protein with a PpiC-isomerase domain. Intriguingly, the putative proline substrate for NifM is present in NifH proteins from strains lacking nifM, raising questions regarding the actual ...
Zhuoting Xie, Shuyi Cai, Haoyang Chen, Shuyuan Kong, Letian Tang, Yi-Ping Wang, Jianguo Yang   +6 more
doaj   +2 more sources

Effects of reduced nitrogen on the nifH-harboring soil microbiome in a soybean-maize strip intercropping system [PDF]

Frontiers in Microbiology
IntroductionNitrogen (N) is a core limiting factor for crop growth, with approximately 50% of global food production relying on chemical N fertilizer inputs.
Fang Liu, Fang Liu, Lisong Shi, Lisong Shi, Shuo Yan, Shuo Yan, Yiling Zhang, Yiling Zhang, Mengxi Zhang, Mengxi Zhang, Tonghao Han, Xuan Zhao, Xuan Zhao, Zhanjun Li, Zhanjun Li, Ning Niu, Ning Niu   +16 more
doaj   +2 more sources

Diversity of nifH Gene in Culturable Rhizobia from Black Locust (Robinia pseudoacacia L.) Grown in Cadmium-Contaminated Soils [PDF]

Biology
(1) Background: Rhizobia can promote plant growth by providing essential nutrients such as NH4+ and PO43−; thus, rhizobia that can tolerate the stress of heavy metals will be conducive to the phytoremediation of heavy-metal-contaminated soils. Therefore,
Xiaomeng Wang, Xia Jia, Yonghua Zhao, Yuan Xie, Xiuxin Meng, Fang Wang   +5 more
doaj   +2 more sources

Enhancing Role of Nitrogen Fixation in Biogeochemical Cycles of the Pacific Arctic. [PDF]

Glob Chang Biol
Composite mean fields of sea surface temperature and sea‐ice distribution for September during 2015–2024, comparing years with high (2015, 2016, 2021) and low (2017, 2019, 2023) sea‐ice coverage. Earlier sea‐ice melt can promote the advection of diazotrophs (UCYN‐A2 and their haptophyte hosts) from the Bering Sea into the Beaufort Sea basin, where ...
Shiozaki T, Fujiwara A, Watanabe E, Nishino S, Harada N, Makabe A.   +5 more
europepmc   +2 more sources

Undervalued Pseudo-nifH Sequences in Public Databases Distort Metagenomic Insights into Biological Nitrogen Fixers

mSphere, 2021
Nitrogen fixation, a distinct process incorporating the inactive atmospheric nitrogen into the active biological processes, has been a major topic in biological and geochemical studies.
Kazumori Mise, Yoko Masuda, Keishi Senoo, Hideomi Itoh   +3 more
doaj   +1 more source