nifH gene expression and diversity in geothermal springs of Tengchong, China [PDF]
Frontiers in Microbiology, 2022 Terrestrial hot springs have been suggested to harbor diverse diazotrophic lineages by using DNA-based nifH gene phylogenetic analysis. However, only a small amount of diazotrophs were ever confirmed to perform nitrogen fixation.
Zhao-Qi Song +6 more
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A comprehensive evaluation of PCR primers to amplify the nifH gene of nitrogenase. [PDF]
PLoS ONE, 2012 The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed
John Christian Gaby, Daniel H Buckley
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Seasonal and long-term effects of nutrient additions and liming on the nifH gene in cerrado soils under native vegetation [PDF]
iScience, 2021 Summary: Biological nitrogen fixation (BNF) represents the main input source of N in tropical savannas. BNF could be particularly important for Brazilian savannas (known as Cerrado) that show a highly conservative N cycle.
Rafaella Silveira +7 more
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Frontiers in Microbiology, 2018 Diazotrophic microorganisms introduce biologically available nitrogen (N) to the global N cycle through the activity of the nitrogenase enzyme. The genetically conserved dinitrogenase reductase (nifH) gene is phylogenetically distributed across four ...
Roey Angel +6 more
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Genetic analysis of the NifM dependence of the nitrogenase iron proteins [PDF]
mBioNifH maturation in certain diazotrophic bacteria depends on NifM, a protein with a PpiC-isomerase domain. Intriguingly, the putative proline substrate for NifM is present in NifH proteins from strains lacking nifM, raising questions regarding the actual ...
Zhuoting Xie +6 more
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Effects of reduced nitrogen on the nifH-harboring soil microbiome in a soybean-maize strip intercropping system [PDF]
Frontiers in MicrobiologyIntroductionNitrogen (N) is a core limiting factor for crop growth, with approximately 50% of global food production relying on chemical N fertilizer inputs.
Fang Liu +16 more
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Diversity of nifH Gene in Culturable Rhizobia from Black Locust (Robinia pseudoacacia L.) Grown in Cadmium-Contaminated Soils [PDF]
Biology(1) Background: Rhizobia can promote plant growth by providing essential nutrients such as NH4+ and PO43−; thus, rhizobia that can tolerate the stress of heavy metals will be conducive to the phytoremediation of heavy-metal-contaminated soils. Therefore,
Xiaomeng Wang +5 more
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mSphere, 2021 Nitrogen fixation, a distinct process incorporating the inactive atmospheric nitrogen into the active biological processes, has been a major topic in biological and geochemical studies.
Kazumori Mise +3 more
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The response of diazotrophs to nutrient amendment in the South China Sea and western North Pacific [PDF]
Biogeosciences, 2022 The availability of iron (Fe) and phosphorus (P) has been shown to be a key factor regulating rates of nitrogen fixation in the western subtropical Pacific.
Z. Wen +13 more
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Nitrogenase gene amplicons from global marine surface waters are dominated by genes of non-cyanobacteria. [PDF]
PLoS ONE, 2011 Cyanobacteria are thought to be the main N(2)-fixing organisms (diazotrophs) in marine pelagic waters, but recent molecular analyses indicate that non-cyanobacterial diazotrophs are also present and active.
Hanna Farnelid +8 more
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