Genetics in methanogens: transposon insertion mutagenesis of a Methanococcus maripaludis nifH gene [PDF]
Journal of Bacteriology, 1995 We designed a transposon insertion mutagenesis system for Methanococcus species and used it to make mutations in and around a nifH gene in Methanococcus maripaludis. The transposon Mudpur was constructed with a gene for puromycin resistance that is expressed and selectable in Methanococcus species. A 15.6-kb nifH region from M.
C E, Blank, P S, Kessler, J A, Leigh
openaire +2 more sources
Showing copy numbers of the nifH mRNA transcript and the nifH gene.
, 2012 nifH mRNA transcripts shown in the top and nifH gene in the bottom panels respectively. Bars labelled with the same letter in the same year are not different (P = 0.05). Unlabelled bars in the same year are not significantly different.
Julia M. Cooper (106318) +3 more
core +1 more source
Phylogenetic dendrogram of strain D5 nifH gene.
, 2013 Phylogenetic dendrogram of strain D5 nifH gene.
Yili Zhao (298495) +3 more
core +1 more source
PCR results of strain D5 nifH gene.
, 2013 PCR results of strain D5 nifH gene.
Yili Zhao (298495) +3 more
core +1 more source
Long-term nitrogen fertilization shaped the nifH, nirK and nosZ gene community patterns in red paddy soil in south China。 [PDF]
, 2020 To understand the diversities of diazotrophs and denitrifiers in red paddy soil under long-term fertilization conditions, nifH, nirK and nosZ libraries were constructed based on the PCR-RFLP method.
Zhang, Cunzhi +13 more
core +1 more source
Comparison ofnifHGene Pools in Soils and Soil Microenvironments with Contrasting Properties [PDF]
Applied and Environmental Microbiology, 2001 ABSTRACTThe similarities and differences in the structures of thenifHgene pools of six different soils (Montrond, LCSA-p, Vernon, Dombes, LCSA-c, and Thysse Kaymor) and five soil fractions extracted from LCSA-c were studied. Bacterial DNA was directly extracted from the soils, and a region of thenifHgene was amplified by PCR and analyzed by restriction.
Poly, F. +4 more
openaire +3 more sources
nifH-based community metrics fail to predict benthic nitrogen fixation rates
, 2018 data acompanying the article "nifH-based community metrics fail to predict benthic nitrogen fixation rates". preprint here: https://peerj.com/manuscripts/17768/
Kristina Sundbäck (294723) +6 more
core +1 more source
Diverse and Novel nifH and nifH -Like Gene Sequences in the Deep-Sea Methane Seep Sediments of the Okhotsk Sea [PDF]
Applied and Environmental Microbiology, 2009 ABSTRACT Diverse nifH and nifH -like gene sequences were obtained from the deep-sea surface sediments of the methane hydrate-bearing Okhotsk Sea. Some sequences formed novel families of the NifH or NifH-like proteins, of currently unresolved bacterial or archaeal origin.
Hongyue, Dang +3 more
openaire +2 more sources
, 1998 The nifH gene sequence of the nitrogen-fixing bacterium Acetobacter diazotrophicus was determined with the use of the polymerase chain reaction and universal degenerate oligonucleotide primers. The gene shows highest pair-wise similarity to the nifH gene
Fegan, M +3 more
core +1 more source
Expression of the Klebsiella pneumoniae nifH gene in Saccharomyces cerevisiae
Enzyme and Microbial Technology, 1987 Dinitrogenase reductase, a component of a complex and highly regulated prokaryotic enzyme, nitrogenase, is expressed in the eukaryote Saccharomyces cerevisiae. A plasmid pH-ADH-1 was constructed that directs the expression of the Klebsiella pneumoniae nifH gene, encoding dinitrogenase reductase, from the yeast alcohol dehydrogenase I promoter.
Claude V. Maina +2 more
openaire +1 more source