Gammaproteobacterial diazotrophs and nifH gene expression in surface waters of the South Pacific Ocean [PDF]
Abstract In addition to the cyanobacterial N2-fixers (diazotrophs), there is a high nifH gene diversity of non-cyanobacterial groups present in marine environments, yet quantitative information about these groups is scarce. N2 fixation potential (nifH gene expression), diversity and distributions of the uncultivated diazotroph ...
Pia Moisander +2 more
exaly +7 more sources
nifH genes in the obligate methane oxidizing bacteria [PDF]
The hifH gene from Klebsiella pneumoniae, which codes for the Fe protein component of nitrogenase, was used as a probe to detect nifH homologues in total cellular DNA from 13 obligate methane oxidizing bacteria. All but one of those strains that had previously been shown capable of fixing dinitrogen contained sequences homologous to nifH.
Christopher J. Oakley, J. Colin Murrell
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Exploration of nifH gene through soil metagenomes of the western Indian Himalayas. [PDF]
This group has previously highlighted the prevalence of Csp genes from cold Himalayan environments. However, this study has explored the uncultured diazotrophs from metagenomes of western Indian Himalayas. The metagenomic nifH gene clone library was constructed from the Temperate, Subtropical and Tarai soils of Western Himalaya, India followed by ...
Soni R, Suyal DC, Sai S, Goel R.
europepmc +4 more sources
Detection and characterization of cyanobacterial nifH genes [PDF]
The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594.
J, Ben-Porath, J P, Zehr
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Comparative phylogeny of rrs and nifH genes in the Bacillaceae [PDF]
The rrs (16S rDNA) gene sequences of nitrogen-fixing endospore-forming bacilli isolated from the rhizosphere of wheat and maize were determined in order to infer their phylogenetic position in the Bacillaceae. These rhizosphere strains form a monophyletic cluster with Paenibacillus azotofixans, Paenibacillus polymyxa and Paenibacillus macerans.
Achouak, Wafa +2 more
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Cloning and sequencing of the nifH gene ofDesulfovibrio gigas [PDF]
The Desulfovibrio gigas nifH gene has been cloned and sequenced. It consists of an open-reading frame of 822 base pairs encoding a 274 amino acid polypeptide. A potential ntrA-dependent promotor sequence is present. The gene lacks an upstream activator sequence homologous to those often found in nif genes subject to activation by nifA.
H M, Kent, M, Buck, D J, Evans
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Constraining uncertainties of diazotroph biogeography from nifH gene abundance [PDF]
AbstractMarine diazotrophs fix dinitrogen gas into bioavailable nitrogen that drives the ocean nitrogen cycle; yet, efforts to infer global diazotroph distributions have been limited by a sparsity of observations. In situ measurements of nifH gene abundance (essential for nitrogen fixation) are increasingly being used to inform the biogeography of ...
Simona Meiler +6 more
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In Rhizobium japonicum the nitrogenase genes nifH and nifDK are separated [PDF]
In contrast to Klebsiella pneumoniae or fast-growing Rhizobium species, such as R. meliloti, where the nitrogenase structural genes are clustered in one operon (nifHDK), in slow-growing Rhizobium japonicum 110, nifH and nifDK are on separate operons.
K, Kaluza +4 more
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Characterization and analysis of nifH genes from Paenibacillus sabinae T27 [PDF]
Paenibacillus sabinae T27 (CCBAU 10202=DSM 17841) is a gram-positive, spore-forming diazotroph with high nitrogenase activities. Three nifH clusters were cloned from P. sabinae T27. Phylogenetic analysis revealed that NifH1, NifH2 and NifH3 cluster with Cyanobacterium. Each of the coding regions of nifH1, nifH2 and nifH3 from P.
Hong, Yuanyuan +5 more
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Rhizobium japonicum nitrogenase Fe protein gene (nifH) [PDF]
A 12.1-kilobase PstI fragment from Rhizobium japonicum, which contains homology to both the Klebsiella pneumoniae and the Rhizobium meliloti nifH genes, was cloned into vector pHE3 . The nifH -homologous region was localized on the restriction enzyme cleavage map by Southern blot hybridization experiments. DNA fragments overlapping the R.
M, Fuhrmann, H, Hennecke
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