A comprehensive evaluation of PCR primers to amplify the nifH gene of nitrogenase. [PDF]
The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed
John Christian Gaby +5 more
core +5 more sources
<i>NifH</i> gene amplicon sequencing and metagenomic approaches are complementary in assessing diazotroph diversity. [PDF]
7 pages, 3 figures, supplementary data https://doi.org/10.1093/ismeco/ycaf038.-- Data availability: The global ocean nifH ASV database (version 1) analyzed in this study is available in Figshare (https://doi.org/10.6084/m9.figshare.23795943.v1)Exploring ...
Cheung S +5 more
europepmc +5 more sources
nifH gene expression and diversity in geothermal springs of Tengchong, China. [PDF]
Terrestrial hot springs have been suggested to harbor diverse diazotrophic lineages by using DNA-based nifH gene phylogenetic analysis. However, only a small amount of diazotrophs were ever confirmed to perform nitrogen fixation.
Song ZQ +6 more
europepmc +4 more sources
The entire coding region of chlL, an essential chloroplast gene required for chlorophyll biosynthesis in the dark in Chlamydomonas reinhardtii, was precisely replaced by either the Klebsiella pneumoniae nifH (encoding the structural component of ...
Dixon, Ray +9 more
core +3 more sources
nifH genes in the obligate methane oxidizing bacteria [PDF]
The hifH gene from Klebsiella pneumoniae, which codes for the Fe protein component of nitrogenase, was used as a probe to detect nifH homologues in total cellular DNA from 13 obligate methane oxidizing bacteria. All but one of those strains that had previously been shown capable of fixing dinitrogen contained sequences homologous to nifH.
Christopher J. Oakley, J. Colin Murrell
openaire +2 more sources
Detection and characterization of cyanobacterial nifH genes [PDF]
The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594.
J, Ben-Porath, J P, Zehr
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Comparative phylogeny of rrs and nifH genes in the Bacillaceae [PDF]
The rrs (16S rDNA) gene sequences of nitrogen-fixing endospore-forming bacilli isolated from the rhizosphere of wheat and maize were determined in order to infer their phylogenetic position in the Bacillaceae. These rhizosphere strains form a monophyletic cluster with Paenibacillus azotofixans, Paenibacillus polymyxa and Paenibacillus macerans.
Achouak, Wafa +2 more
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Isolation of Glucose Fermenting Nitrogen Fixing Gene (nifH) Containing Endophytic Bacteria from Seed and Roots of Wheat (Triticum aestivation l.) [PDF]
The present study was conducted to study the nifH gene containing endophytic bacterial frequency in wheat seed and roots obtained from the soil of Chitwan and Kaski. One hundred and four isolates were studied for the presence of the nifH gene.
Yadav, Pankaj Kumar +6 more
core +2 more sources
Cloning and sequencing of the nifH gene ofDesulfovibrio gigas [PDF]
The Desulfovibrio gigas nifH gene has been cloned and sequenced. It consists of an open-reading frame of 822 base pairs encoding a 274 amino acid polypeptide. A potential ntrA-dependent promotor sequence is present. The gene lacks an upstream activator sequence homologous to those often found in nif genes subject to activation by nifA.
H M, Kent, M, Buck, D J, Evans
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DETECTION OF THE NIFH GENE IN NITROGEN FIXING BACTERIA FROM AGRICULTURAL AREAS IN ERZURUM
Plant Growth Promoting Rhizospheric Bacteria (PGPR) are one of the most potential biofertilizer agents in agricultural applications. Biofertilizers have been used instead of chemical fertilizers last a few decades in the all over world because of their ...
GÜLLÜCE, Medine +2 more
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