Results 1 to 10 of about 8,213 (179)

A comprehensive evaluation of PCR primers to amplify the nifH gene of nitrogenase. [PDF]

PLoS ONE, 2012
The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed
John Christian Gaby, Daniel H Buckley
doaj   +2 more sources

Spatiotemporal Regulation of N₂ Fixation and Photosynthesis in Trichodesmium: New Insights From mRNA CARD-FISH and nanoSIMS. [PDF]

Environ Microbiol
Examining the coordination of the O2‐sensitive N2 fixation process with photosynthesis at single‐cell level in the filamentous Trichodesmium, we find spatio‐temporal separation of N2 fixation and photosynthesis at gene expression and fixation level as well as indications for rapid intercellular transport followed by co‐localized storage of C and N ...
Rebeca LA, Anxhela H, Anežka M, Michiel K, Kasia P, Ondřej P, Ľuboš P, Meri E.   +7 more
europepmc   +2 more sources

Genetic analysis of the NifM dependence of the nitrogenase iron proteins [PDF]

mBio
NifH maturation in certain diazotrophic bacteria depends on NifM, a protein with a PpiC-isomerase domain. Intriguingly, the putative proline substrate for NifM is present in NifH proteins from strains lacking nifM, raising questions regarding the actual ...
Zhuoting Xie, Shuyi Cai, Haoyang Chen, Shuyuan Kong, Letian Tang, Yi-Ping Wang, Jianguo Yang   +6 more
doaj   +2 more sources

Biotechnological potential of Pectobacterium sp. endophyte on the growth of soy and bean plants

Revista Principia, 2023
Fertilizers and chemical pesticides supply plants’ nutritional requirements and protect them against pathogens. However, they may cause harm to the environment. Pectobacterium sp.
João Arthur dos Santos Oliveira, Natieli Jenifer Mateus, Ana Paula Ferreira, Helio Conte, João Lucio de Azevedo   +4 more
doaj   +1 more source

Undervalued Pseudo-nifH Sequences in Public Databases Distort Metagenomic Insights into Biological Nitrogen Fixers

mSphere, 2021
Nitrogen fixation, a distinct process incorporating the inactive atmospheric nitrogen into the active biological processes, has been a major topic in biological and geochemical studies.
Kazumori Mise, Yoko Masuda, Keishi Senoo, Hideomi Itoh   +3 more
doaj   +1 more source

Analysis of Nitrogenase Fe Protein Activity in Transplastomic Tobacco

Frontiers in Agronomy, 2021
Integration of prokaryotic nitrogen fixation (nif) genes into the plastid genome for expression of functional nitrogenase components could render plants capable of assimilating atmospheric N2 making their crops less dependent of nitrogen fertilizers. The
Jose A. Aznar-Moreno, Xi Jiang, Xi Jiang, Stefan Burén, Luis M. Rubio, Luis M. Rubio   +5 more
doaj   +1 more source

Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.).

PLoS ONE, 2023
Chemical nitrogen fertilizer can maintain crop productivity, but overuse of chemical nitrogen fertilizers leads to economic costs and environmental pollution.
Yimin Shang, Wenfang Guo, Xiaomeng Liu, Lei Ma, Dehu Liu, Sanfeng Chen   +5 more
doaj   +2 more sources

Assembly of nitrogenase biosynthetic pathway in Saccharomyces cerevisiae by using polyprotein strategy

Frontiers in Microbiology, 2023
Nitrogenase in some bacteria and archaea catalyzes conversion of N2 to ammonia. To reconstitute a nitrogenase biosynthetic pathway in a eukaryotic host is still a challenge, since synthesis of nitrogenase requires a large number of nif (nitrogen fixation)
Minyang Wang, Yimin Shang, Xiaomeng Liu, Sanfeng Chen   +3 more
doaj   +1 more source

Widespread Distribution and Expression of Gamma A (UMB), an Uncultured, Diazotrophic, γ-Proteobacterial nifH Phylotype. [PDF]

PLoS ONE, 2015
Marine dinitrogen (N2) fixation studies have focused nearly exclusively on cyanobacterial diazotrophs; however γ-proteobacteria are an abundant component of the marine community and have been largely overlooked until recently.
Rebecca Langlois, Tobias Großkopf, Matthew Mills, Shigenobu Takeda, Julie LaRoche   +4 more
doaj   +1 more source

Elevated Atmospheric CO2 and Nitrogen Fertilization Affect the Abundance and Community Structure of Rice Root-Associated Nitrogen-Fixing Bacteria

Frontiers in Microbiology, 2021
Elevated atmospheric CO2 (eCO2) results in plant growth and N limitation, yet how root-associated nitrogen-fixing bacterial communities respond to increasing atmospheric CO2 and nitrogen fertilization (eN) during the growth stages of rice is unclear ...
Jumei Liu, Jumei Liu, Jumei Liu, Jingjing Han, Chunwu Zhu, Weiwei Cao, Weiwei Cao, Ying Luo, Meng Zhang, Shaohua Zhang, Zhongjun Jia, Ruihong Yu, Ruihong Yu, Ji Zhao, Ji Zhao, Zhihua Bao, Zhihua Bao   +16 more
doaj   +1 more source