Results 1 to 10 of about 5,029 (176)
A comprehensive evaluation of PCR primers to amplify the nifH gene of nitrogenase. [PDF]
PLoS ONE, 2012 The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed
John Christian Gaby, Daniel H Buckley
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Genetic analysis of the NifM dependence of the nitrogenase iron proteins [PDF]
mBioNifH maturation in certain diazotrophic bacteria depends on NifM, a protein with a PpiC-isomerase domain. Intriguingly, the putative proline substrate for NifM is present in NifH proteins from strains lacking nifM, raising questions regarding the actual ...
Zhuoting Xie +6 more
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Frontiers in Microbiology, 2018 Diazotrophic microorganisms introduce biologically available nitrogen (N) to the global N cycle through the activity of the nitrogenase enzyme. The genetically conserved dinitrogenase reductase (nifH) gene is phylogenetically distributed across four ...
Dagmar Woebken +2 more
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NifH-Harboring Bacterial Community Composition across an Alaskan Permafrost Thaw Gradient
Frontiers in Microbiology, 2016 Since nitrogen (N) is often limiting in permafrost soils, we investigated the N2-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples taken along a permafrost thaw gradient in an Alaskan boreal
Feifei Liu, Ye Deng, James Tiedje
exaly +3 more sources
Biotechnological potential of Pectobacterium sp. endophyte on the growth of soy and bean plants
Revista Principia, 2023 Fertilizers and chemical pesticides supply plants’ nutritional requirements and protect them against pathogens. However, they may cause harm to the environment. Pectobacterium sp.
João Arthur dos Santos Oliveira +4 more
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mSphere, 2021 Nitrogen fixation, a distinct process incorporating the inactive atmospheric nitrogen into the active biological processes, has been a major topic in biological and geochemical studies.
Kazumori Mise +3 more
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Analysis of Nitrogenase Fe Protein Activity in Transplastomic Tobacco
Frontiers in Agronomy, 2021 Integration of prokaryotic nitrogen fixation (nif) genes into the plastid genome for expression of functional nitrogenase components could render plants capable of assimilating atmospheric N2 making their crops less dependent of nitrogen fertilizers. The
Jose A. Aznar-Moreno +5 more
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Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.).
PLoS ONE, 2023 Chemical nitrogen fertilizer can maintain crop productivity, but overuse of chemical nitrogen fertilizers leads to economic costs and environmental pollution.
Yimin Shang +5 more
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Frontiers in Microbiology, 2023 Nitrogenase in some bacteria and archaea catalyzes conversion of N2 to ammonia. To reconstitute a nitrogenase biosynthetic pathway in a eukaryotic host is still a challenge, since synthesis of nitrogenase requires a large number of nif (nitrogen fixation)
Minyang Wang +3 more
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Widespread Distribution and Expression of Gamma A (UMB), an Uncultured, Diazotrophic, γ-Proteobacterial nifH Phylotype. [PDF]
PLoS ONE, 2015 Marine dinitrogen (N2) fixation studies have focused nearly exclusively on cyanobacterial diazotrophs; however γ-proteobacteria are an abundant component of the marine community and have been largely overlooked until recently.
Rebecca Langlois +4 more
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