Results 11 to 20 of about 6,727 (173)

A comprehensive evaluation of PCR primers to amplify the nifH gene of nitrogenase.

PLoS ONE, 2012
The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed
John Christian Gaby, Daniel H. Buckley (51969), Daniel H Buckley, John Christian Gaby (148535)   +3 more
core   +5 more sources

Minimal ATP-Independent N₂-Reducing Systems Defined by L-Cluster-Bound Nitrogenase Assembly Platforms. [PDF]

Angew Chem Int Ed Engl
Association of the L‐cluster with the nitrogenase assembly proteins NifEN (NifENL) or NifB (NifBL) intrinsically endows these proteins with N2‐reducing activity, enabling in vitro N2‐reduction by NifENL and NifBL when supplied with chemical reductants or photoexcited quantum dots while supporting in vivo N2‐fixation in NifENL‐ and NifENL‐expressing ...
Quechol R, Yang Y, Lee CC, Ribbe MW, Hu Y.   +4 more
europepmc   +2 more sources

Sediments as Potential Sources of Non-Cyanobacterial Diazotrophs in Arctic Sea Ice and Seawater. [PDF]

Environ Microbiol Rep
Diazotrophic communities across Arctic environments: Integrated analyses of diazotrophs in sea ice, seawater, and sediments show that Arctic continental shelf sediments function as a central reservoir, supplying diazotrophs to both sea ice and seawater through resuspension processes.
Bo H, Zhang Z, Fujiwara A, Fukai Y, Ito M, Kimura S, Oggier M, Waga H, Whitmore L, Hamasaki K, Shiozaki T.   +10 more
europepmc   +2 more sources

Contamination of diversenifHandnifH-like DNA into commercial PCR primers [PDF]

FEMS Microbiology Letters, 2005
False-positive results due to DNA contamination in PCR reagents have become a big problem in the amplification of small amounts of DNA. Recently, it was revealed that PCR reagents were contaminated with the nifH (dinitrogenase reductase) gene. We found that the PCR primers supplied by some manufacturers contained nifH gene and nifH-like DNA.
Masahiro, Goto, Shotaro, Ando, Yusuke, Hachisuka, Tadakatsu, Yoneyama   +3 more
openaire   +2 more sources

Characterization of nifH mutations of Klebsiella pneumoniae [PDF]

Journal of Bacteriology, 1988
Nucleotide changes in the nifH gene of Klebsiella pneumoniae were identified by DNA cloning and sequencing of six selected mutant strains. The strains were UN60, C-640-GC----TGC; UN116, C-67-TC----TTC; UN117, G-688-AG----AAG; UN1041, CG-302-C----CAC; UN1678, GC-713-C----GTC; and UN1795, G-439-AG----AAG. Their corresponding amino acid substitutions were
C L, Chang, L C, Davis, M, Rider, D J, Takemoto   +3 more
openaire   +2 more sources

Detection and characterization of cyanobacterial nifH genes [PDF]

Applied and Environmental Microbiology, 1994
The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594.
J, Ben-Porath, J P, Zehr
openaire   +2 more sources

Cloning and sequencing of the nifH gene ofDesulfovibrio gigas [PDF]

FEMS Microbiology Letters, 1989
The Desulfovibrio gigas nifH gene has been cloned and sequenced. It consists of an open-reading frame of 822 base pairs encoding a 274 amino acid polypeptide. A potential ntrA-dependent promotor sequence is present. The gene lacks an upstream activator sequence homologous to those often found in nif genes subject to activation by nifA.
H M, Kent, M, Buck, D J, Evans
openaire   +2 more sources

Discovery of Eremiobacterota with nifH homologs in tundra soil

Environmental Microbiology Reports, 2023
Abstract We describe the genome of an Eremiobacterota population from tundra soil that contains the minimal set of nif genes needed to fix atmospheric N 2 . This putative diazotroph population, which we name Candidatus
Pessi, Igor, Delmont, Tom, Zehr, Jonathan, Hultman, Jenni   +3 more
openaire   +6 more sources

Inhibition of Iron-Molybdenum Cofactor Biosynthesis by L127Δ NifH and Evidence for a Complex Formation between L127Δ NifH and NifNE [PDF]

Journal of Biological Chemistry, 1999
Besides serving as the obligate electron donor to dinitrogenase during nitrogenase turnover, dinitrogenase reductase (NifH) is required for the biosynthesis of the iron-molybdenum cofactor (FeMo-co) and for the maturation of alpha(2)beta(2) apo-dinitrogenase (apo-dinitrogenase maturation).
P, Rangaraj, M J, Ryle, W N, Lanzilotta, P J, Goodwin, D R, Dean, V K, Shah, P W, Ludden   +6 more
openaire   +2 more sources

Enhancing Role of Nitrogen Fixation in Biogeochemical Cycles of the Pacific Arctic. [PDF]

Glob Chang Biol
Composite mean fields of sea surface temperature and sea‐ice distribution for September during 2015–2024, comparing years with high (2015, 2016, 2021) and low (2017, 2019, 2023) sea‐ice coverage. Earlier sea‐ice melt can promote the advection of diazotrophs (UCYN‐A2 and their haptophyte hosts) from the Bering Sea into the Beaufort Sea basin, where ...
Shiozaki T, Fujiwara A, Watanabe E, Nishino S, Harada N, Makabe A.   +5 more
europepmc   +2 more sources