Results 11 to 20 of about 12,024 (210)
NifH-Harboring Bacterial Community Composition across an Alaskan Permafrost Thaw Gradient [PDF]
Frontiers in Microbiology, 2016 Since nitrogen (N) is often limiting in permafrost soils, we investigated the N2-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples taken along a permafrost thaw gradient in an Alaskan boreal
Christopher Ryan Penton +2 more
exaly +7 more sources
Genetic analysis of the NifM dependence of the nitrogenase iron proteins [PDF]
mBioNifH maturation in certain diazotrophic bacteria depends on NifM, a protein with a PpiC-isomerase domain. Intriguingly, the putative proline substrate for NifM is present in NifH proteins from strains lacking nifM, raising questions regarding the actual ...
Zhuoting Xie +6 more
doaj +2 more sources
A Comprehensive Evaluation of PCR Primers to Amplify the nifH Gene of Nitrogenase [PDF]
PLoS ONE, 2012 The nifH gene is the most widely sequenced marker gene used to identify nitrogen-fixing Bacteria and Archaea. Numerous PCR primers have been designed to amplify nifH, but a comprehensive evaluation of nifH PCR primers has not been performed. We performed
John Christian Gaby, Daniel H Buckley
exaly +2 more sources
Frontiers in Microbiology, 2018 Diazotrophic microorganisms introduce biologically available nitrogen (N) to the global N cycle through the activity of the nitrogenase enzyme. The genetically conserved dinitrogenase reductase (nifH) gene is phylogenetically distributed across four ...
Roey Angel +2 more
exaly +3 more sources
Biotechnological potential of Pectobacterium sp. endophyte on the growth of soy and bean plants
Revista Principia, 2023 Fertilizers and chemical pesticides supply plants’ nutritional requirements and protect them against pathogens. However, they may cause harm to the environment. Pectobacterium sp.
João Arthur dos Santos Oliveira +4 more
doaj +1 more source
mSphere, 2021 Nitrogen fixation, a distinct process incorporating the inactive atmospheric nitrogen into the active biological processes, has been a major topic in biological and geochemical studies.
Kazumori Mise +3 more
doaj +1 more source
Analysis of Nitrogenase Fe Protein Activity in Transplastomic Tobacco
Frontiers in Agronomy, 2021 Integration of prokaryotic nitrogen fixation (nif) genes into the plastid genome for expression of functional nitrogenase components could render plants capable of assimilating atmospheric N2 making their crops less dependent of nitrogen fertilizers. The
Jose A. Aznar-Moreno +5 more
doaj +1 more source
Contamination of diversenifHandnifH-like DNA into commercial PCR primers [PDF]
FEMS Microbiology Letters, 2005 False-positive results due to DNA contamination in PCR reagents have become a big problem in the amplification of small amounts of DNA. Recently, it was revealed that PCR reagents were contaminated with the nifH (dinitrogenase reductase) gene. We found that the PCR primers supplied by some manufacturers contained nifH gene and nifH-like DNA.
Masahiro, Goto +3 more
openaire +2 more sources
Constraining uncertainties of diazotroph biogeography from nifH gene abundance [PDF]
Limnology and Oceanography, 2022 AbstractMarine diazotrophs fix dinitrogen gas into bioavailable nitrogen that drives the ocean nitrogen cycle; yet, efforts to infer global diazotroph distributions have been limited by a sparsity of observations. In situ measurements of nifH gene abundance (essential for nitrogen fixation) are increasingly being used to inform the biogeography of ...
Simona Meiler +6 more
openaire +2 more sources
Co-expression of nitrogenase proteins in cotton (Gossypium hirsutum L.).
PLoS ONE, 2023 Chemical nitrogen fertilizer can maintain crop productivity, but overuse of chemical nitrogen fertilizers leads to economic costs and environmental pollution.
Yimin Shang +5 more
doaj +2 more sources