Results 11 to 20 of about 5,029 (176)
Minimal ATP-Independent N<sub>2</sub>-Reducing Systems Defined by L-Cluster-Bound Nitrogenase Assembly Platforms. [PDF]
Angew Chem Int Ed EnglAssociation of the L‐cluster with the nitrogenase assembly proteins NifEN (NifENL) or NifB (NifBL) intrinsically endows these proteins with N2‐reducing activity, enabling in vitro N2‐reduction by NifENL and NifBL when supplied with chemical reductants or photoexcited quantum dots while supporting in vivo N2‐fixation in NifENL‐ and NifENL‐expressing ...
Quechol R +4 more
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Contamination of diversenifHandnifH-like DNA into commercial PCR primers [PDF]
FEMS Microbiology Letters, 2005 False-positive results due to DNA contamination in PCR reagents have become a big problem in the amplification of small amounts of DNA. Recently, it was revealed that PCR reagents were contaminated with the nifH (dinitrogenase reductase) gene. We found that the PCR primers supplied by some manufacturers contained nifH gene and nifH-like DNA.
Masahiro, Goto +3 more
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Characterization of nifH mutations of Klebsiella pneumoniae [PDF]
Journal of Bacteriology, 1988 Nucleotide changes in the nifH gene of Klebsiella pneumoniae were identified by DNA cloning and sequencing of six selected mutant strains. The strains were UN60, C-640-GC----TGC; UN116, C-67-TC----TTC; UN117, G-688-AG----AAG; UN1041, CG-302-C----CAC; UN1678, GC-713-C----GTC; and UN1795, G-439-AG----AAG. Their corresponding amino acid substitutions were
C L, Chang +3 more
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Detection and characterization of cyanobacterial nifH genes [PDF]
Applied and Environmental Microbiology, 1994 The DNA sequence of a 359-bp fragment of nifH was determined for the heterocystous strains Anabaena sp. strain CA (ATCC 33047), Nostoc muscorum UTEX 1933, a Nostoc sp., Gloeothece sp. strain ATCC 27152, Lyngbya lagerheimii UTEX 1930, and Plectonema boryanum IU 594.
J, Ben-Porath, J P, Zehr
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Comparative phylogeny of rrs and nifH genes in the Bacillaceae [PDF]
International Journal of Systematic and Evolutionary Microbiology, 1999 The rrs (16S rDNA) gene sequences of nitrogen-fixing endospore-forming bacilli isolated from the rhizosphere of wheat and maize were determined in order to infer their phylogenetic position in the Bacillaceae. These rhizosphere strains form a monophyletic cluster with Paenibacillus azotofixans, Paenibacillus polymyxa and Paenibacillus macerans.
Achouak, Wafa +2 more
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Cloning and sequencing of the nifH gene ofDesulfovibrio gigas [PDF]
FEMS Microbiology Letters, 1989 The Desulfovibrio gigas nifH gene has been cloned and sequenced. It consists of an open-reading frame of 822 base pairs encoding a 274 amino acid polypeptide. A potential ntrA-dependent promotor sequence is present. The gene lacks an upstream activator sequence homologous to those often found in nif genes subject to activation by nifA.
H M, Kent, M, Buck, D J, Evans
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Discovery of Eremiobacterota with nifH homologs in tundra soil
Environmental Microbiology Reports, 2023 Abstract We describe the genome of an Eremiobacterota population from tundra soil that contains the minimal set of nif genes needed to fix atmospheric N 2 . This putative diazotroph population, which we name Candidatus
Pessi, Igor +3 more
openaire +6 more sources
Inhibition of Iron-Molybdenum Cofactor Biosynthesis by L127Δ NifH and Evidence for a Complex Formation between L127Δ NifH and NifNE [PDF]
Journal of Biological Chemistry, 1999 Besides serving as the obligate electron donor to dinitrogenase during nitrogenase turnover, dinitrogenase reductase (NifH) is required for the biosynthesis of the iron-molybdenum cofactor (FeMo-co) and for the maturation of alpha(2)beta(2) apo-dinitrogenase (apo-dinitrogenase maturation).
P, Rangaraj +6 more
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nifH amplification for Illumina sequencing v1 [PDF]
, 2020 For metabarcoding purpose, the first step involves the amplification by PCR of a given gene region (for example V4 or V9 region of 18S rRNA gene) or gene itself if its size does not exceed 600bp (the longest fragment size that can be sequenced by Illumina technology).
Estelle Bigeard +2 more
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Acta Biologica Slovenica, 2022 Selection of genes that have not been horizontally transferred for prokaryote phylogenetic studies is regarded as a challenging task. Internal transcribed spacer of ribosomal genes (16S–23S ITS), microcystin synthetase genes (mcy), nitrogenase (nifH) and
Bahareh Nowruzi
doaj +1 more source