Results 21 to 30 of about 6,727 (173)

nifH amplification for Illumina sequencing v1 [PDF]

, 2020
For metabarcoding purpose, the first step involves the amplification by PCR of a given gene region (for example V4 or V9 region of 18S rRNA gene) or gene itself if its size does not exceed 600bp (the longest fragment size that can be sequenced by Illumina technology).
Estelle Bigeard, Adriana Lopes Dos Santos, Catherine Ribeiro   +2 more
openaire   +1 more source

In Rhizobium japonicum the nitrogenase genes nifH and nifDK are separated [PDF]

Journal of Bacteriology, 1983
In contrast to Klebsiella pneumoniae or fast-growing Rhizobium species, such as R. meliloti, where the nitrogenase structural genes are clustered in one operon (nifHDK), in slow-growing Rhizobium japonicum 110, nifH and nifDK are on separate operons.
K, Kaluza, M, Fuhrmann, M, Hahn, B, Regensburger, H, Hennecke   +4 more
openaire   +2 more sources

The Abundance of the nifH Gene Became Higher and the nifH-Containing Diazotrophic Bacterial Communities Changed During Primary Succession in the Hailuogou Glacier Chronosequence, China [PDF]

Frontiers in Microbiology, 2021
Primary successional ecosystems and the related soil development are often N limited. To date, N2-fixing communities during primary succession in alpine ecosystems have remained underexplored. In this study, we applied quantitative PCR (qPCR) quantitation and targeted amplicon sequencing of nifH in the Hailuogou Glacier foreland to investigate the ...
Yingyan Wang, Yulan Chen, Qinyu Xue, Quanju Xiang, Ke Zhao, Xiumei Yu, Qiang Chen, Menggen Ma, Hao Jiang, Xiaoping Zhang, Petri Penttinen, Yunfu Gu   +11 more
openaire   +3 more sources

Rhizobium japonicum nitrogenase Fe protein gene (nifH) [PDF]

Journal of Bacteriology, 1984
A 12.1-kilobase PstI fragment from Rhizobium japonicum, which contains homology to both the Klebsiella pneumoniae and the Rhizobium meliloti nifH genes, was cloned into vector pHE3 . The nifH -homologous region was localized on the restriction enzyme cleavage map by Southern blot hybridization experiments. DNA fragments overlapping the R.
M, Fuhrmann, H, Hennecke
openaire   +2 more sources

Nitrogen fixation and nifH diversity in human gut microbiota [PDF]

Scientific Reports, 2016
AbstractIt has been hypothesized that nitrogen fixation occurs in the human gut. However, whether the gut microbiota truly has this potential remains unclear. We investigated the nitrogen-fixing activity and diversity of the nitrogenase reductase (NifH) genes in the faecal microbiota of humans, focusing on Papua New Guinean and Japanese individuals ...
Katsura Igai, Manabu Itakura, Suguru Nishijima, Hiroto Tsurumaru, Wataru Suda, Takumi Tsutaya, Eriko Tomitsuka, Kiyoshi Tadokoro, Jun Baba, Shingo Odani, Kazumi Natsuhara, Ayako Morita, Minoru Yoneda, Andrew R. Greenhill1, Paul F. Horwood, Jun-Ichi Inoue, Moriya Ohkuma, Yuichi Hongoh, Taro Yamamoto, Peter Siba, Masahira Hattori, Kiwamu Minamisawa, Masahiro Umezaki   +22 more
openaire   +3 more sources

Diverse and Novel nifH and nifH -Like Gene Sequences in the Deep-Sea Methane Seep Sediments of the Okhotsk Sea [PDF]

Applied and Environmental Microbiology, 2009
ABSTRACT Diverse nifH and nifH -like gene sequences were obtained from the deep-sea surface sediments of the methane hydrate-bearing Okhotsk Sea. Some sequences formed novel families of the NifH or NifH-like proteins, of currently unresolved bacterial or archaeal origin.
Hongyue, Dang, Xiwu, Luan, Jingyi, Zhao, Jing, Li   +3 more
openaire   +2 more sources

nifH gene expression and diversity in geothermal springs of Tengchong, China

Frontiers in Microbiology, 2022
Terrestrial hot springs have been suggested to harbor diverse diazotrophic lineages by using DNA-based nifH gene phylogenetic analysis. However, only a small amount of diazotrophs were ever confirmed to perform nitrogen fixation. In order to explore the compositions of active diazotrophic populations in hot springs, the in situ expression and diversity
Zhao-Qi Song, Li Wang, Feng Liang, Qingfeng Zhou, Dongli Pei, Hongchen Jiang, Wen-Jun Li   +6 more
openaire   +3 more sources

Constraining uncertainties of diazotroph biogeography from nifH gene abundance [PDF]

Limnology and Oceanography, 2022
AbstractMarine diazotrophs fix dinitrogen gas into bioavailable nitrogen that drives the ocean nitrogen cycle; yet, efforts to infer global diazotroph distributions have been limited by a sparsity of observations. In situ measurements of nifH gene abundance (essential for nitrogen fixation) are increasingly being used to inform the biogeography of ...
Simona Meiler, Gregory L. Britten, Stephanie Dutkiewicz, Mary Rose Gradoville, Pia H. Moisander, Oliver Jahn, Michael J. Follows   +6 more
openaire   +2 more sources

Nucleotide sequence of the R.meliloti nitrogenase reductase (nifH) gene

Nucleic Acids Research, 1981
The nucleotide sequence of the structural gene (nifH) of nitrogenase reductase (Fe protein) from R.meliloti 41 with its flanking ends is reported. The amino acid sequence of nitrogenase reductase was deduced from the DNA sequence. The predicted R.meliloti nitrogenase reductase protein consists of 297 amino acid residues, has a molecular weight of 32 ...
I, Török, A, Kondorosi
openaire   +3 more sources

NifH and NifD sequences of heliobacteria: a new lineage in the nitrogenase phylogeny [PDF]

FEMS Microbiology Letters, 2005
We determined almost complete nifH and nifD genes from representatives of all recognized genera of heliobacteria, the strictly anaerobic phototrophs belonging to the low GC gram-positive bacteria. The heliobacterial sequences formed a highly supported monophyletic group that is clearly distinct from any known diazotrophs, in both NifH and NifD trees ...
Jigjiddorj, Enkh-Amgalan, Hiroko, Kawasaki, Tatsuji, Seki   +2 more
openaire   +2 more sources