Results 51 to 60 of about 4,632 (165)

Expression of the Klebsiella pneumoniae nifH gene in Saccharomyces cerevisiae

open access: yesEnzyme and Microbial Technology, 1987
Dinitrogenase reductase, a component of a complex and highly regulated prokaryotic enzyme, nitrogenase, is expressed in the eukaryote Saccharomyces cerevisiae. A plasmid pH-ADH-1 was constructed that directs the expression of the Klebsiella pneumoniae nifH gene, encoding dinitrogenase reductase, from the yeast alcohol dehydrogenase I promoter.
Claude V. Maina   +2 more
openaire   +1 more source

Seasonal Shifts in Diazotrophs Players: Patterns Observed Over a Two-Year Time Series in the New Caledonian Lagoon (Western Tropical South Pacific Ocean)

open access: yesFrontiers in Marine Science, 2020
Coastal and open ocean regions of the Western Tropical South Pacific ocean have been identified as a hotspot of N2 fixation. However, the environmental factors driving the temporal variability of abundance, composition, and activity of diazotrophs are ...
Emmrick Saulia   +14 more
doaj   +1 more source

A novel strain D5 isolated from Acacia confusa. [PDF]

open access: yesPLoS ONE, 2012
We isolated a novel strain D5 from nodules of Acacia confusa. Under strict sterile conditions the strain could successfully nodulate Acacia confusa, A. crassicarpa and A.
Baoling Huang   +3 more
doaj   +1 more source

Relative abundance of nitrogen cycling microbes in coral holobionts reflects environmental nitrate availability

open access: yesRoyal Society Open Science, 2021
Recent research suggests that nitrogen (N) cycling microbes are important for coral holobiont functioning. In particular, coral holobionts may acquire bioavailable N via prokaryotic dinitrogen (N2) fixation or remove excess N via denitrification activity.
Arjen Tilstra   +6 more
doaj   +1 more source

Diagnosis and Management of Prenatal Hereditary Pyropoikilocytosis

open access: yesPrenatal Diagnosis, EarlyView.
ABSTRACT Hereditary pyropoikilocytosis (HPP) is a severe hemolytic anemia caused by variants in SPTA1, SPTB, and EPB41. These weaken horizontal interactions in the erythrocyte cytoskeleton, causing membrane fragmentation and splenic sequestration. It will readily cause fetal anemia and often hydrops fetalis. Prenatal diagnosis requires first ruling out
Connor Hartzell   +6 more
wiley   +1 more source

Nodularia sp. nifH gene transcripts in the Baltic Sea proper [PDF]

open access: yesJournal of Plankton Research, 2007
While bacterioplankton indisputably control vital biogeochemical paths in the cycling of carbon and nutrients in the world’s oceans, our knowledge about the functional and genetic diversity of bacterioplankton communities is negligible. In this thesis, molecular and more traditional microbiological methods were used to study the specific function of N2-
Kjärstin H. Boström   +3 more
openaire   +1 more source

Structure and functional genes associated with carbon, nitrogen, phosphorus, and sulfur cycling processes in periphyton: A comparison between natural and artificial rivers

open access: yesRiver, EarlyView.
Network analysis of functional genes among prokaryotes, and eukaryotes in natural and artificial rivers. Abstract Periphyton in aquatic ecosystems plays vital roles in the elemental cycle process and is vulnerable to anthropogenic interference. However, few studies have explored the elemental cycles of carbon (C), nitrogen (N), phosphorus (P), and ...
Yulu Tian   +4 more
wiley   +1 more source

Molecular evidence for sediment nitrogen fixation in a temperate New England estuary [PDF]

open access: yesPeerJ, 2016
Primary production in coastal waters is generally nitrogen (N) limited with denitrification outpacing nitrogen fixation (N2-fixation). However, recent work suggests that we have potentially underestimated the importance of heterotrophic sediment N2 ...
Silvia E. Newell   +3 more
doaj   +2 more sources

Enhanced CO2 Coordinates the Spatial Recruitment of Diazotrophs in Rice Via Root Development

open access: yesPlant, Cell &Environment, Volume 49, Issue 7, Page 3678-3693, July 2026.
ABSTRACT Understanding the reciprocal interaction between root development and coadapted beneficial microbes in response to elevated CO2 (eCO2) will facilitate the identification of nutrient‐efficient cultivars for sustainable agriculture. Here, systematic morphological, anatomical, chemical and gene expression assays performed under low‐nitrogen ...
Junwen Zhao   +12 more
wiley   +1 more source

Development and Testing of a DNA Macroarray To Assess Nitrogenase ( nifH ) Gene Diversity [PDF]

open access: yesApplied and Environmental Microbiology, 2004
ABSTRACT A DNA macroarray was developed and evaluated for its potential to distinguish variants of the dinitrogenase reductase ( nifH ) gene. Diverse nifH gene fragments amplified from a clone library were spotted onto nylon membranes. Amplified, biotinylated nifH
Grieg F, Steward   +3 more
openaire   +2 more sources

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