Results 51 to 60 of about 4,632 (165)
Expression of the Klebsiella pneumoniae nifH gene in Saccharomyces cerevisiae
Dinitrogenase reductase, a component of a complex and highly regulated prokaryotic enzyme, nitrogenase, is expressed in the eukaryote Saccharomyces cerevisiae. A plasmid pH-ADH-1 was constructed that directs the expression of the Klebsiella pneumoniae nifH gene, encoding dinitrogenase reductase, from the yeast alcohol dehydrogenase I promoter.
Claude V. Maina +2 more
openaire +1 more source
Coastal and open ocean regions of the Western Tropical South Pacific ocean have been identified as a hotspot of N2 fixation. However, the environmental factors driving the temporal variability of abundance, composition, and activity of diazotrophs are ...
Emmrick Saulia +14 more
doaj +1 more source
A novel strain D5 isolated from Acacia confusa. [PDF]
We isolated a novel strain D5 from nodules of Acacia confusa. Under strict sterile conditions the strain could successfully nodulate Acacia confusa, A. crassicarpa and A.
Baoling Huang +3 more
doaj +1 more source
Recent research suggests that nitrogen (N) cycling microbes are important for coral holobiont functioning. In particular, coral holobionts may acquire bioavailable N via prokaryotic dinitrogen (N2) fixation or remove excess N via denitrification activity.
Arjen Tilstra +6 more
doaj +1 more source
Diagnosis and Management of Prenatal Hereditary Pyropoikilocytosis
ABSTRACT Hereditary pyropoikilocytosis (HPP) is a severe hemolytic anemia caused by variants in SPTA1, SPTB, and EPB41. These weaken horizontal interactions in the erythrocyte cytoskeleton, causing membrane fragmentation and splenic sequestration. It will readily cause fetal anemia and often hydrops fetalis. Prenatal diagnosis requires first ruling out
Connor Hartzell +6 more
wiley +1 more source
Nodularia sp. nifH gene transcripts in the Baltic Sea proper [PDF]
While bacterioplankton indisputably control vital biogeochemical paths in the cycling of carbon and nutrients in the world’s oceans, our knowledge about the functional and genetic diversity of bacterioplankton communities is negligible. In this thesis, molecular and more traditional microbiological methods were used to study the specific function of N2-
Kjärstin H. Boström +3 more
openaire +1 more source
Network analysis of functional genes among prokaryotes, and eukaryotes in natural and artificial rivers. Abstract Periphyton in aquatic ecosystems plays vital roles in the elemental cycle process and is vulnerable to anthropogenic interference. However, few studies have explored the elemental cycles of carbon (C), nitrogen (N), phosphorus (P), and ...
Yulu Tian +4 more
wiley +1 more source
Molecular evidence for sediment nitrogen fixation in a temperate New England estuary [PDF]
Primary production in coastal waters is generally nitrogen (N) limited with denitrification outpacing nitrogen fixation (N2-fixation). However, recent work suggests that we have potentially underestimated the importance of heterotrophic sediment N2 ...
Silvia E. Newell +3 more
doaj +2 more sources
Enhanced CO2 Coordinates the Spatial Recruitment of Diazotrophs in Rice Via Root Development
ABSTRACT Understanding the reciprocal interaction between root development and coadapted beneficial microbes in response to elevated CO2 (eCO2) will facilitate the identification of nutrient‐efficient cultivars for sustainable agriculture. Here, systematic morphological, anatomical, chemical and gene expression assays performed under low‐nitrogen ...
Junwen Zhao +12 more
wiley +1 more source
Development and Testing of a DNA Macroarray To Assess Nitrogenase ( nifH ) Gene Diversity [PDF]
ABSTRACT A DNA macroarray was developed and evaluated for its potential to distinguish variants of the dinitrogenase reductase ( nifH ) gene. Diverse nifH gene fragments amplified from a clone library were spotted onto nylon membranes. Amplified, biotinylated nifH
Grieg F, Steward +3 more
openaire +2 more sources

