Results 131 to 140 of about 3,203,913 (163)

Nucleotide Sequence of the Gene Encoding the Nitrogenase Iron Protein (nifH) of Azospirillum Brasilense and Identification of a Region Controlling nifH Transcription

Molecular Genetics and Genomics, 1989
The DNA sequence was determined for the Azospirillum brasilense nifH gene and part of the nifD gene. The nifH gene is 885 bp long and encodes 293 amino acid residues. The region upstream of the nifH open reading frame contains a putative promoter whose sequence shows perfect homology with promoters of other diazotrophic bacteria and two putative ...
Renato Fani, M Bazzicalupo, M Polsinelli
exaly   +4 more sources

EndophyticnifHgene diversity in African sweet potato

Canadian Journal of Microbiology, 2003
A cultivation-independent approach was used to identify potentially nitrogen-fixing endophytes in seven sweet potato varieties collected in Uganda and Kenya. Nitrogenase reductase genes (nifH) were amplified by PCR, and amplicons were cloned in Escherichia coli.
Birgit, Reiter, Helmut, Bürgmann, Kornel, Burg, Angela, Sessitsch   +3 more
openaire   +2 more sources

A Study of Nucleotide Sequences of nifH Genes of Some Methanotrophic Bacteria

Microbiology, 2002
Using a previously developed primer system, nifH gene fragments 450 nucleotides long were amplified, cloned, and sequenced for representatives of nitrogen-fixing methanotrophic bacteria of the genera Methylococcus, Methylocystis, and Methylosinus. Fragments of nifH genes were also detected and sequenced in representatives of the genera Methylomonas and
B B Kuznetsov, T V Kolganova, E S Boulygina   +2 more
exaly   +2 more sources

Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil

Journal of Environmental Sciences, 2007
Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis (DGGE) and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination.
Zhen-Cheng, Su, Hui-Wen, Zhang, Xin-Yu, Li, Qin, Zhang, Cheng-Gang, Zhang   +4 more
openaire   +2 more sources

In Rhizobium Japonicum the Nifh and Nifdk Genes are Separated

1984
We have shown previously that a cloned HindIII restriction fragment from Rhizobium japonicum 3I1b 110 contains the nitrogenase structural genes nifD and nifK (Fig. 1). However, we have not obtained evidence for the existence of the gene for nitrogenase reductase (nifH) adjacent to th nifDK cluster.
Martin Fuhrmann, Klaus Kaluza, Hauke Hennecke   +2 more
openaire   +1 more source

Diazotrophic Diversity, nifH Gene Expression and Nitrogenase Activity in a Rice Paddy Field in Fujian, China [PDF]

Plant and Soil, 2009
The diazotrophic communities in a rice paddy field were characterized by a molecular polyphasic approach including DNA/RNA-DGGE fingerprinting, real time RT-PCR analysis of nifH gene and the measurement of nitrogen fixation activities.
Beatriz Díez, Rehab El-Shehawy
exaly   +2 more sources

Distribution of Extensive nifH Gene Diversity Across Physical Soil Microenvironments

Microbial Ecology, 2006
The diversity of nitrogen-fixing bacteria is well described for aquatic environments; however, terrestrial analyses remain mostly biased to rhizobial plant-microbe associations. We maximized the level of resolution for this study through the use of nucleotide sequence information extracted from a series of soil microenvironments, ranging from ...
Izquierdo, JA, Nusslein, K
openaire   +2 more sources

The nitrogen-fixing gene (nifH) of Rhodopseudomonas palustris: a case of lateral gene transfer?

Microbiology, 2004
Nitrogen fixation is catalysed by some photosynthetic bacteria. This paper presents a phylogenetic comparison of a nitrogen fixation gene (nifH) with the aim of elucidating the processes underlying the evolutionary history of Rhodopseudomonas palustris. In the NifH phylogeny, strains of Rps.
Jose Jason L, Cantera, Hiroko, Kawasaki, Tatsuji, Seki   +2 more
openaire   +2 more sources

Quantitative Analysis of nifH Genes and Transcripts from Aquatic Environments

2005
The availability of fixed inorganic nitrogen often plays a fundamental role in regulating primary production in both aquatic and terrestrial ecosystems. Because biological nitrogen fixation is an important source of nitrogen in marine environments, the study of N2-fixing microorganisms is of fundamental importance to our understanding of global ...
Steven M, Short, Jonathan P, Zehr
openaire   +2 more sources

Detection and quantification of the nifH gene in shoot and root of cucumber plants

Canadian Journal of Microbiology, 2006
A real-time polymerase chain reaction (PCR) method was applied to quantify the nifH gene pool in cucumber shoot and root and to evaluate how nitrogen (N) supply and plant age affect the nifH gene pool. In shoots, the relative abundance of the nifH gene was affected neither by different stages of plant growth nor by N supply.
Dilafruz, Juraeva, Eckhard, George, Kahramon, Davranov, Silke, Ruppel   +3 more
openaire   +2 more sources