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Nucleotide sequence of the gene encoding the nitrogenase iron protein (nifH) of Azospirillum brasilense and identification of a region controlling nifH transcription

Molecular Genetics and Genomics, 1989
The DNA sequence was determined for the Azospirillum brasilense nifH gene and part of the nifD gene. The nifH gene is 885 bp long and encodes 293 amino acid residues. The region upstream of the nifH open reading frame contains a putative promoter whose sequence shows perfect homology with promoters of other diazotrophic bacteria and two putative ...
Renato Fani   +2 more
exaly   +4 more sources

Transcription analysis of the three nifH genes of Rhizobium phaseoli with gene fusions

Molecular Genetics and Genomics, 1988
Rhizobium phaseoli CFN42 possesses three regions containing genes coding for the nitrogenase polypeptides: region a and region b contain copies of the three nitrogenase structural genes nifH, nifD and nifK and region c contains only a nifH gene.
Enrique Morett   +2 more
exaly   +2 more sources

The cloning and functional characterization of the nifH gene of Rhodospirillum rubrum

Gene, 1990
Dinitrogenase reductase (the nifH product) from Rhodospirillum rubrum is regulated by a post-translational modification system encoded by draTG. As demonstrated in this report, the cloning, sequencing, and functional characterization of the nifH gene provides a basis for further analysis as well as revealing interesting features of gene organization ...
L J, Lehman   +2 more
openaire   +2 more sources

EndophyticnifHgene diversity in African sweet potato

Canadian Journal of Microbiology, 2003
A cultivation-independent approach was used to identify potentially nitrogen-fixing endophytes in seven sweet potato varieties collected in Uganda and Kenya. Nitrogenase reductase genes (nifH) were amplified by PCR, and amplicons were cloned in Escherichia coli.
Birgit, Reiter   +3 more
openaire   +2 more sources

Toxic effects of acetochlor, methamidophos and their combination on nifH gene in soil

Journal of Environmental Sciences, 2007
Toxic effects of two agrochemicals on nifH gene in agricultural black soil were investigated using denaturing gradient gel electrophoresis (DGGE) and sequencing approaches in a microcosm experiment. Changes of soil nifH gene diversity and composition were examined following the application of acetochlor, methamidophos and their combination.
Zhen-Cheng, Su   +4 more
openaire   +2 more sources

Two seasons’ study on nifH gene expression and nitrogen fixation by diazotrophic endophytes in sugarcane (Saccharum spp. hybrids): expression of nifH genes similar to those of rhizobia

Plant and Soil, 2010
To explore the presence and expression of the nifH gene of diazotrophic endophytes in sugarcane (Saccharum spp. hybrids) cv. NiF8, we conducted pot experiments for two seasons in a glasshouse from 10 June to 17 September (high temperature and long days) in 2005 and 1 September to 9 December (low temperature and short days) in 2006.
Shotaro Ando   +2 more
exaly   +2 more sources

Distribution of Extensive nifH Gene Diversity Across Physical Soil Microenvironments

Microbial Ecology, 2006
The diversity of nitrogen-fixing bacteria is well described for aquatic environments; however, terrestrial analyses remain mostly biased to rhizobial plant-microbe associations. We maximized the level of resolution for this study through the use of nucleotide sequence information extracted from a series of soil microenvironments, ranging from ...
Izquierdo, JA, Nusslein, K
openaire   +2 more sources

In Rhizobium Japonicum the Nifh and Nifdk Genes are Separated

1984
We have shown previously that a cloned HindIII restriction fragment from Rhizobium japonicum 3I1b 110 contains the nitrogenase structural genes nifD and nifK (Fig. 1). However, we have not obtained evidence for the existence of the gene for nitrogenase reductase (nifH) adjacent to th nifDK cluster.
Martin Fuhrmann   +2 more
openaire   +1 more source

The nitrogen-fixing gene (nifH) of Rhodopseudomonas palustris: a case of lateral gene transfer?

Microbiology, 2004
Nitrogen fixation is catalysed by some photosynthetic bacteria. This paper presents a phylogenetic comparison of a nitrogen fixation gene (nifH) with the aim of elucidating the processes underlying the evolutionary history of Rhodopseudomonas palustris. In the NifH phylogeny, strains of Rps.
Jose Jason L, Cantera   +2 more
openaire   +2 more sources

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