Results 71 to 80 of about 2,342 (296)

Controlling Agricultural Emissions of Nitrates: Regulations versus Taxes [PDF]

, 2017
Two policy instruments, input taxes and regulations, can be used to deal with nitrate pollution. However, in practice command and control (CAC) measures such as input regulations and management practices, as outlined in Action Programmes under the EU ...
Lally, Breda, Riordan, Brendan, van Rensburg, Tom M.   +2 more
core   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Development of human monoclonal antibodies against TARM1 by yeast display

FEBS Open Bio, EarlyView.
Human monoclonal antibodies against TARM1 are generated by yeast display‐guided selection. These antibodies bind to soluble and cell‐surface forms of TARM1. Also, these antibodies exhibit agonistic activity in the NFAT‐GFP reporter assay, indicating that TARM1 signaling can be functionally modulated by antibodies and suggesting TARM1 as a potential ...
Rikio Yabe, Mayumi Saeki, Masaaki Hashiguchi, Sayaka Ono, Kazuhisa Aoki, Hidetaka Tanno   +5 more
wiley   +1 more source

Costs of compliance with EU regulations and competitiveness of the EU dairy sector

, 2008
The introduction of cross-compliance mechanism in the European Union with its 2003 CAPreform might affect the costs of production and thus competitiveness of the EU.
Bezlepkina, I., Meister, A., de Roest, K., Brouwer, F.M., Roest, K., de, Dillen, K., Jongeneel, R., Winsten, J., Demont, M.   +8 more
core  

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Analysing a Bottom Up Methodology for Developing Communal Biogas Plants in Croatia

Journal of Sustainable Development of Energy, Water and Environment Systems, 2015
This research presents a bottom up methodology for assessing biogas potentials applied in a local community in Croatia. The research is based on analysing the flow of resources on a local level and capability of local actors to innovate and cooperate ...
Mak Đukan, Zoran Aralica
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

The potential to reduce the risk of diffuse pollution from agriculture while improving economic performance at farm level

, 2013
peer-reviewedWithin the constraints of the EU Nitrates and Water Framework Directives, controlling and managing nutrient transfers to water from excessive nutrient use on agricultural land is a significant environmental policy challenge.
Buckley, Cathal, Carney, Patricia
core   +1 more source

Mise en relation de l'évolution de l'agriculture et de la qualité de l'eau entre 1950 et 2000 [PDF]

Biotechnologie, Agronomie, Société et Environnement, 2010
Relationship between evolution in the agriculture and in the water quality from 1950 to 2000. In 1991, Europe has adopted the Nitrates Directive to reduce the impact of agriculture on water nitrate pollution.
Vandenberghe C.
doaj  

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

FEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke, Michal Banasik, Rokas Juodeikis, Martin J. Warren, Richard W. Pickersgill   +4 more
wiley   +1 more source