Results 251 to 260 of about 251,937 (291)

[44] Nitrite reductase

1971
Publisher Summary This chapter discusses the determination of nitrite reductase. The usual assay involves sodium dithionite as reductant and either ferredoxin or its artificial substitute, methyl viologen, as the electron carrier. Enzymatic activity can be best followed by measuring colorimetrically the rate of disappearance of nitrite.
Antonio Paneque, M. Losada
openaire   +2 more sources

Crystal structure of the soluble domain of the major anaerobically induced outer membrane protein (AniA) from pathogenic Neisseria: a new class of copper-containing nitrite reductases.

Journal of Molecular Biology, 2002
The major anaerobically induced outer membrane protein (AniA) from pathogenic Neisseria gonorrhoeae is essential for cell growth under oxygen limiting conditions in the presence of nitrite and is protective against killing by human sera.
M. Boulanger, M. Murphy
semanticscholar   +1 more source

A nitrite reductase from Neurospora crassa

Biochimica et Biophysica Acta, 1960
Abstract Nitrite reductase has been purified, over 50-fold from ordinary felts of Neurospora crassa, macroconidial wild type Em 5297a. The enzyme is a DPNH-dependent flavoprotein containing FAD, Fe and Cu and -SH groups. Copper may act by coupling the flavin component of the enzyme to nitrite since an external supply of Cu1+ reduced NO2 non ...
D. J. D. Nicholas, Owen T.G. Jones, Antonia Medina   +2 more
openaire   +3 more sources

Electron donation between copper containing nitrite reductases and cupredoxins: the nature of protein-protein interaction in complex formation.

Journal of Molecular Biology, 2002
In denitrifying organisms with copper containing dissimilatory nitrite reductases, electron donation from a reduced cupredoxin is an essential step in the reduction of nitrite to nitric oxide.
L. Murphy, F. E. Dodd, F. Yousafzai, R. Eady, S. Hasnain   +4 more
semanticscholar   +1 more source

Regulation of nitrite uptake and nitrite reductase expression in Chlamydomonas reinhardtii

Biochimica et Biophysica Acta (BBA) - General Subjects, 1991
Expression of nitrite uptake and nitrite reductase activities has been studied in Chlamydomonas reinhardtii under different nutritional conditions. Both activities were expressed at a low level in derepressed cells (with no nitrogen source) and at a high level in induced cells (with nitrate or nitrite).
Jacobo Cárdenas, Emilio Fernández, Francisco Córdoba, Aurora Galván   +3 more
openaire   +3 more sources

The anoxic plant mitochondrion as a nitrite: NO reductase

Mitochondrion, 2011
Under the conditions of oxygen deprivation, accumulating nitrite can be reduced in the mitochondrial electron transport chain forming free radical nitric oxide (NO). By reducing nitrite to NO, plant mitochondria preserve the capacity to oxidize external NADH and NADPH and retain a limited power for ATP synthesis complementing glycolytic ATP production.
Abir U. Igamberdiev, Kapuganti Jagadis Gupta   +1 more
openaire   +3 more sources

Ferredoxin-nitrite reductase from spinach

Biochimica et Biophysica Acta (BBA) - Enzymology and Biological Oxidation, 1966
Summary 1. Nitrite reductase from a crude homogenate of spinach leaves has been purified about 500-fold and freed from NADP-reductase (EC 1.6.99.4) and nitrate reductase (EC 1.6.6.2). The enzyme, which does not seem to be a flavoprotein, catalyzes the reduction of nitrite to ammonia with a variety of enzyme systems as electron donors and requires
F.F. Del Campo, Manuel Losada, Antonio Paneque, Juan M. Ramírez   +3 more
openaire   +3 more sources

[23] Ferredoxin-nitrite reductase

1980
Publisher Summary This chapter describes ferredoxin-nitrite reductase. Ferredoxin-nitrite reductase (EC 1.7.7.1) is the second enzyme component of the photosynthetic nitrate-reducing system. It was first identified as a ferredoxin-dependent chloroplast enzyme which catalyzes the 6-electron reduction of nitrite to ammonia.
Jacobo Cárdenas, José M. Vega, M. Losada   +2 more
openaire   +2 more sources

Stoichiometry of nitrite reduction catalyzed by Pseudomonas aeruginosa nitrite-reductase

Biochimie, 1984
The stoichiometry of the reduction of nitrite catalyzed by Pseudomonas aeruginosa nitrite-reductase (cytochrome cd1) has been shown to yield nitrous oxide as the final product. Gas chromatography experiments demonstrated that nitric oxide is also formed as a free intermediate. A sequential formation of NO and N2O is discussed as opposed to the parallel
Yann Henry, Philippe Bessières
openaire   +3 more sources

Characterization of two type 1 Cu sites of Hyphomicrobium denitrificans nitrite reductase: a new class of copper-containing nitrite reductases.

Biochemistry, 2004
We report (1) the amino acid sequence of Hyphomicrobium denitrificans nitrite reductase (HdNIR), containing two type 1 Cu sites and one type 2 Cu site; (2) the expression and preparation of wild-type HdNIR and two mutants replacing the Cys ligand of each
K. Yamaguchi, K. Kataoka, M. Kobayashi, K. Itoh, A. Fukui, Shinnichiro Suzuki   +5 more
semanticscholar   +1 more source