Results 91 to 100 of about 1,099,567 (306)

Evidence of chemical exchange in recombinant Major Urinary Protein and quenching thereof upon pheromone binding [PDF]

, 2018
The internal dynamics of recombinant Major Urinary Protein (rMUP) have been investigated by monitoring transverse nitrogen-15 relaxation using multiple-echo Carr-Purcell-Meiboom-Gill (CPMG) experiments.
Bodenhausen, Geoffrey, Homans, Steve, Perazzolo, Chiara, Verde, Mariachiara   +3 more
core  

Confirmation of co-denitrification in grazed grassland [PDF]

, 2015
peer-reviewedPasture-based livestock systems are often associated with losses of reactive forms of nitrogen (N) to the environment. Research has focused on losses to air and water due to the health, economic and environmental impacts of reactive N.
Cameron, K.C., Clough, Timothy J., Di, H.J., Grant, Jim, Lanigan, Gary, Laughlin, Ronald J., Moir, James L., Richards, Karl G., Selbie, Diana R., Somers, Cathal, Watson, C. J.   +10 more
core   +1 more source

C2α‐carbanion‐protonating glutamate discloses tradeoffs between substrate accommodation and reaction rate in actinobacterial 2‐hydroxyacyl‐CoA lyase

FEBS Open Bio, EarlyView.
Enzymes of the 2‐hydroxyacyl‐CoA lyase group catalyze the condensation of formyl‐CoA with aldehydes or ketones. Thus, by structural adaptation of active sites, practically any pharmaceutically and industrially important 2‐hydroxyacid could be biotechnologically synthesized. Combining crystal structure analysis, active site mutations and kinetic assays,
Michael Zahn, Barbara Seroka, Ryszard Lazny, Zenon Lotowski, Thore Rohwerder   +4 more
wiley   +1 more source

Nitrogen-15 dynamic nuclear polarization of nicotinamide derivatives in biocompatible solutions. [PDF]

Sci Adv, 2023
Peters JP, Brahms A, Janicaud V, Anikeeva M, Peschke E, Ellermann F, Ferrari A, Hellmold D, Held-Feindt J, Kim NM, Meiser J, Aden K, Herges R, Hövener JB, Pravdivtsev AN.   +14 more
europepmc   +1 more source

PARP inhibitors induce a senescence phenotype in non‐small cell lung carcinoma cell lines

FEBS Open Bio, EarlyView.
Talazoparib is the most potent inducer of senescence among different PARP1 inhibitors in human NSCLC cells. In the absence of PARP, no senescence phenotype was observed, demonstrating that PARP1 is necessary for the induction of senescence by this inhibitor.
Camille Huart, Manon Van den Abbeel, Christoph Schifflers, Karim Bouhjar, Andrea Scarmelotto, Alexis Khelfi, Anne‐Catherine Wera, Carine Michiels   +7 more
wiley   +1 more source

Shifts in the Efficiency of ¹⁵N-Ammonium Sulfate Fertilization to Sugarcane Varieties Inoculated with Diazotrophic Bacteria

Agronomy
Nitrogen is an essential plant nutrient, but N fertilization contributes to greenhouse gas emissions through its production and application as well as nitrous oxide emissions when applied to soil.
Edevaldo de Castro Monteiro, Carolina Almada Gomes de Oliveira, Cleudison Gabriel Nascimento da Silva, Mayan Blanc Amaral, Veronica Massena Reis, Robert Michael Boddey, Bruno José Rodrigues Alves, Segundo Urquiaga   +7 more
doaj   +1 more source

Potential uptake of dissolved organic matter by seagrasses and macroalgae [PDF]

, 2011
Dissolved organic nitrogen (DON) acts as a large reservoir of fixed nitrogen. Whereas DON utilization is common in the microbial community, little is known about utilization by macrophytes.
Bouma, Tjeerd J., Brun Murillo, Fernando Guillermo, Hendriks, Iris E., Lara, Miguel, Middelburg, Jack J., Morris, Edward P., Peralta González, Gloria, Soetaert, Karline, Van Engeland, Tom   +8 more
core   +5 more sources

Erythropoietin modulates hepatic inflammation, glucose homeostasis, and soluble epoxide hydrolase and epoxides in high‐fat diet‐induced obese mice

FEBS Open Bio, EarlyView.
Erythropoietin administration suppresses hepatic soluble epoxide hydrolase (sEH) expression, leading to increased CYP‐derived epoxides. This is associated with a shift in hepatic macrophage polarization characterized by reduced M1 markers and increased M2 markers, along with reduced hepatic inflammation, suppressed hepatic lipogenesis, and attenuated ...
Takeshi Goda, Satoru Sugimoto, Chiharu Cho, Madoka Konishi, Nozomi Inoue, Satoshi Miyagaki, Yasuhiro Kawabe, Takuro Okamura, Masahide Hamaguchi, Hisakazu Nakajima, Michiaki Fukui, Masakazu Shinohara, Tomoko Iehara   +12 more
wiley   +1 more source

Subsecond Three-Dimensional Nitrogen-15 Magnetic Resonance Imaging Facilitated by Parahydrogen-Based Hyperpolarization. [PDF]

J Phys Chem Lett, 2022
Trepakova AI, Skovpin IV, Chukanov NV, Salnikov OG, Chekmenev EY, Pravdivtsev AN, Hövener JB, Koptyug IV.   +7 more
europepmc   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source